Study of laser fluorescence spectroscopy in livers of rats with hypothermic ischemia

Study of laser fluorescence spectroscopy in livers of rats with hypothermic ischemia

After partial hepatectomy (PH), the remaining liver (RL) undergoes regenerative response proportional to the host. Limited literature exists on hepatic viability after tissue injury during hypothermic preservation. Spectroscopy measures cellular fluorescence and is explored for tissue characterizati...

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Bibliographic Details
Published in:Acta Cirúrgica Brasileira Vol. 38; p. e386023
Main Authors: Tsuge, Allison Takeo, Pereira, Jaqueline de Jesus, Vollet-Filho, José Dirceu, Kubrusly, Márcia Saldanha, Galvão, Flávio Henrique Ferreira, Ribeiro, Orlando Nascimento, Moreno, Camila Rodrigues, Ikegami, Renata Nishiyama, Chaib, Eleazar, Castro E Silva, Orlando de
Format: Journal Article
Language:English
Published: Brazil Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 01-01-2023
Subjects:
Animals
Fluorescence
Hepatectomy
Hepatectomy - methods
Ischemia
Ischemia - metabolism
Ki-67 Antigen - metabolism
Lasers
Liver
Liver - metabolism
Liver - surgery
Liver Regeneration - physiology
Rats
Rats, Wistar
Spectrometry, Fluorescence
Spectrum Analysis
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Summary:After partial hepatectomy (PH), the remaining liver (RL) undergoes regenerative response proportional to the host. Limited literature exists on hepatic viability after tissue injury during hypothermic preservation. Spectroscopy measures cellular fluorescence and is explored for tissue characterization and parameter investigation. This study aimed to assess fluorescence analysis (spectroscopy) in evaluating liver viability and its relationship with hepatic tissue regeneration 24 hours after PH. Additionally, we analyzed liver regeneration in RL after 70% partial hepatectomy under hypothermic conditions with laser irradiation. Fifty-six Wistar rats were divided into four groups: total non-perfused liver (control), total perfused liver, partial hepatectomy "in situ", and partial hepatectomy "ex situ". Tissue analysis was performed at 0 and 24 hours using spectroscopy with laser devices emitting at 532 (green) and 405 nm (violet). Spectroscopy identified tissue viability based on consistent results with Ki67 staining. The fluorescence spectra and Ki67 analysis displayed similar patterns, linking proliferative activity and absorption intensity. Fluorescence spectroscopy proves to be promising for real-time analysis of cellular activity and viability. Metabolic activity was observed in groups of live animals and hypothermically preserved samples, indicating cellular function even under blood deprivation and hypothermic conditions.
ISSN:0102-8650
1678-2674
DOI:10.1590/acb386023