Characteristics of sphingomyelin metabolism in the MCF7 and BT474 radiotherapy‑resistant HER2‑positive breast cancer cell lines

Characteristics of sphingomyelin metabolism in the MCF7 and BT474 radiotherapy‑resistant HER2‑positive breast cancer cell lines

Breast cancer is the most common cancer globally in terms of incidence. This cancer is classified into subtypes based on histological or immunological characteristics. HER2-positive cases account for 15-25% of breast cancer cases, and one of the first events in breast carcinogenesis is HER2 upregula...

Full description

Saved in:
Bibliographic Details
Published in:Oncology letters Vol. 28; no. 4; p. 1
Main Authors: Kikuchi, Megumi, Monzen, Satoru, Horikoshi, Mai, Tsuda, Shuri, Tatara, Yota, Wojcik, Andrzej, Mariya, Yasushi
Format: Journal Article
Language:English
Published: Athens Spandidos Publications 01-10-2024
Spandidos Publications UK Ltd
D.A. Spandidos
Subjects:
Breast cancer
Cancer therapies
Cell culture
Cell cycle
Epidermal growth factor
Flow cytometry
Health aspects
Immunology
Lipid metabolism
Penicillin
Physiological aspects
Polymerase chain reaction
Proteins
Radiation therapy
Software
Sphingolipids
Statistical analysis
Japan
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Breast cancer is the most common cancer globally in terms of incidence. This cancer is classified into subtypes based on histological or immunological characteristics. HER2-positive cases account for 15-25% of breast cancer cases, and one of the first events in breast carcinogenesis is HER2 upregulation. Furthermore, HER2 expression increases the detection rate of metastatic or recurrent breast cancers by 50-80%. The epidermal growth factor receptor family includes HER2, which is a transmembrane receptor protein. In our previous case report, patients who were resistant to anti-HER2 monoclonal antibody therapy, chemotherapy and radiotherapy had higher concentrations of phospholipid metabolites such as phosphatidylcholine and sphingomyelin (SM), which was associated with cancer recurrence progression. To better understand the relationship between radiotherapy resistance and SM expression, breast cancer cell lines with and without HER2 expression (MCF7 and BT474) after exposure to ionizing radiation (IR) were examined. In the cell culture supernatant, similar levels of SM in MCF7 cells were identified after 1-4 Gy exposure. However, SM levels in BT474 cells were upregulated compared with those of in the control group. Intracellular SM levels were upregulated in BT474 cells exposed to 1 and 4 Gy compared with the non-irradiated control group. Furthermore, significantly increased mRNA expression levels of sphingomyelin synthase 2 (SGMS2) in BT474 cells exposed to IR were observed compared with those in nonirradiated cells; however, the SGMS2 levels in MCF7 cells did not differ significantly among the 0, 2 and 4 Gy groups. These findings suggested that a higher dose of IR induced the secretion of SM and its associated gene expression in HER2-positive breast cancer cells. Key words: HER2-positive breast cancer, sphingomyelin, sphingomyelin synthase 1, sphingomyelin synthase 2, radioresistant
ISSN:1792-1074
1792-1082
DOI:10.3892/ol.2024.14604