Search Results - "Tsuchiya, Dai"
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rRNA transcription is integral to phase separation and maintenance of nucleolar structure
Published in PLoS genetics (28-08-2023)“…Transcription of ribosomal RNA (rRNA) by RNA Polymerase (Pol) I in the nucleolus is necessary for ribosome biogenesis, which is intimately tied to cell growth…”
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Positive feedback of NDT80 expression ensures irreversible meiotic commitment in budding yeast
Published in PLoS genetics (01-06-2014)“…In budding yeast, meiotic commitment is the irreversible continuation of the developmental path of meiosis. After reaching meiotic commitment, cells finish…”
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3
Defining a core configuration for human centromeres during mitosis
Published in Nature communications (01-12-2023)“…The centromere components cohesin, CENP-A, and centromeric DNA are essential for biorientation of sister chromatids on the mitotic spindle and accurate sister…”
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Dynamic regulation and requirement for ribosomal RNA transcription during mammalian development
Published in Proceedings of the National Academy of Sciences - PNAS (02-08-2022)“…Ribosomal RNA (rRNA) transcription by RNA polymerase I (Pol I) is a critical rate-limiting step in ribosome biogenesis, which is essential for cell survival…”
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5
Liver-derived cell lines from cavefish Astyanax mexicanus as an in vitro model for studying metabolic adaptation
Published in Scientific reports (16-06-2022)“…Cell lines have become an integral resource and tool for conducting biological experiments ever since the Hela cell line was first developed (Scherer et al. in…”
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Repeated evolution of circadian clock dysregulation in cavefish populations
Published in PLoS genetics (12-07-2021)“…Circadian rhythms are nearly ubiquitous throughout nature, suggesting they are critical for survival in diverse environments. Organisms inhabiting largely…”
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Spatial signals link exit from mitosis to spindle position
Published in eLife (11-05-2016)“…In budding yeast, if the spindle becomes mispositioned, cells prevent exit from mitosis by inhibiting the mitotic exit network (MEN). The MEN is a signaling…”
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Adaptation to low parasite abundance affects immune investment and immunopathological responses of cavefish
Published in Nature ecology & evolution (01-10-2020)“…Reduced parasitic infection rates in the developed world are suspected to underlie the rising prevalence of autoimmune disorders. However, the long-term…”
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NOTCH Signaling Controls Ciliary Body Morphogenesis and Secretion by Directly Regulating Nectin Protein Expression
Published in Cell reports (Cambridge) (12-01-2021)“…Anterior segment dysgenesis is often associated with cornea diseases, cataracts, and glaucoma. In the anterior segment, the ciliary body (CB) containing inner…”
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Step-by-step preparation of mouse eye sections for routine histology, immunofluorescence, and RNA in situ hybridization multiplexing
Published in STAR protocols (17-12-2021)“…It can be challenging to maintain tissue integrity using established histology protocols. Here, we describe a protocol composed of Hartman’s fixation, window…”
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A molecular cell biology toolkit for the study of meiosis in the silkworm Bombyx mori
Published in G3 : genes - genomes - genetics (02-05-2023)“…Abstract Meiosis is usually described as 4 essential and sequential processes: (1) homolog pairing; (2) synapsis, mediated by the synaptonemal complex; (3)…”
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spread of a transposon insertion in Rec8 is associated with obligate asexuality in Daphnia
Published in Proceedings of the National Academy of Sciences - PNAS (17-01-2012)“…Although transitions from sexual to asexual reproduction are thought to have important evolutionary consequences, little is known about the mechanistic…”
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Localization and Function of Budding Yeast CENP-A Depends upon Kinetochore Protein Interactions and Is Independent of Canonical Centromere Sequence
Published in Cell reports (Cambridge) (24-12-2014)“…In many eukaryotes, the centromere is epigenetically specified and not strictly defined by sequence. In contrast, budding yeast has a specific 125 bp sequence…”
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14
Cytological karyotyping of three Cochliobolus spp. by the germ tube burst method
Published in Phytopathology (01-04-2001)“…ABSTRACT Cytological karyotypes with mitotic metaphase chromosomes were analyzed for Cochliobolus heterostrophus, C. carbonum, and C. sativus by the germ tube…”
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Autophagy of an Amyloid-like Translational Repressor Regulates Meiotic Exit
Published in Developmental cell (27-01-2020)“…We explored the potential for autophagy to regulate budding yeast meiosis. Following pre-meiotic DNA replication, we blocked autophagy by chemical inhibition…”
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16
Bub3 promotes Cdc20-dependent activation of the APC/C in S. cerevisiae
Published in The Journal of cell biology (25-05-2015)“…The spindle checkpoint ensures accurate chromosome segregation by sending a signal from an unattached kinetochore to inhibit anaphase onset. Numerous studies…”
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c-Myc promotes polyploidy in murine trophoblast cells and suppresses senescence
Published in Placenta (Eastbourne) (07-09-2023)Get full text
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Myc promotes polyploidy in murine trophoblast cells and suppresses senescence
Published in Development (Cambridge) (01-06-2023)“…The placenta is essential for reproductive success. The murine placenta includes polyploid giant cells that are crucial for its function. Polyploidy occurs…”
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Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. Cerevisiae
Published in Journal of visualized experiments (11-10-2022)“…Time-lapse fluorescence microscopy has revolutionized the understanding of meiotic cell-cycle events by providing temporal and spatial data that is often not…”
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Multiple reorganizations of the lateral elements of the synaptonemal complex facilitate homolog segregation in Bombyx mori oocytes
Published in Current biology (22-01-2024)“…Although Lepidopteran females build a synaptonemal complex (SC) in pachytene, homologs do not crossover, necessitating an alternative method of homolog…”
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