Third Harmonic Generation microscopy as a reliable diagnostic tool for evaluating lipid body modification during cell activation: The example of BV-2 microglia cells

Third Harmonic Generation microscopy as a reliable diagnostic tool for evaluating lipid body modification during cell activation: The example of BV-2 microglia cells

Nonlinear optical processes have found widespread applications in fields ranging from fundamental physics to biomedicine. In this study, we attempted to evaluate cell activation by using the Third Harmonic Generation (THG) imaging microscopy as a new diagnostic tool. The BV-2 microglia cell line wit...

Full description

Saved in:
Bibliographic Details
Published in:Journal of structural biology Vol. 189; no. 2; pp. 105 - 113
Main Authors: Gavgiotaki, E., Filippidis, G., Kalognomou, M., Tsouko, A.A., Skordos, I., Fotakis, C., Athanassakis, I.
Format: Journal Article
Language:English
Published: United States Elsevier Inc 01-02-2015
Subjects:
Activation
Animals
Cell Line
Imaging
Lipid bodies
Lipid Droplets - ultrastructure
Mice
Microglia - physiology
Microglia - ultrastructure
Microglia cells
Microscopy, Fluorescence, Multiphoton
Nonlinear microscopy
Third Harmonic Generation
Nonlinear microscopy
Lipid bodies
Microglia cells
Activation
Third Harmonic Generation
Imaging
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Nonlinear optical processes have found widespread applications in fields ranging from fundamental physics to biomedicine. In this study, we attempted to evaluate cell activation by using the Third Harmonic Generation (THG) imaging microscopy as a new diagnostic tool. The BV-2 microglia cell line with or without activation by lipopolysaccharide was chosen as a representative biological model. The results showed that THG imaging could discriminate between the control versus activated state of BV-2 cells not only as to THG signal intensity but also as to THG signal area, while verifying once more that the majority of the intracellular detected signal corresponds to lipid bodies. Since THG imaging is a real time, non-destructive modality and does not require any prior cell processing and staining, the results presented here provide an important tool for normal versus activated cell discrimination, which could be proved very useful in the study of inflammation.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1047-8477
1095-8657
DOI:10.1016/j.jsb.2014.11.011