Characterization, occurrence, and molecular cloning of a 39-kilodalton Brucella abortus cytoplasmic protein immunodominant in cattle

Monoclonal antibodies and polyclonal antisera recognizing a 39-kDa protein (P39) of brucellin, a cytoplasmic extract from Brucella melitensis rough strain B115, were produced. The P39 was purified by anion-exchange chromatography. Eleven of fourteen Brucella-infected cows whose infections had been d...

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Bibliographic Details
Published in:	Infection and Immunity Vol. 65; no. 2; pp. 495 - 502
Main Authors:	Denoel, P.A. (Facultes Universitaires Notre-Dame de la Paix, Namur, Belgium.), Vo, T.K.O, Tibor, A, Weynants, V.E, Trunde, J.M, Dubray, G, Limet, J.N, Letesson, J.J
Format:	Journal Article
Language:	English
Published:	Washington, DC American Society for Microbiology 01-02-1997
Subjects:	Allergens - administration & dosage Allergens - chemistry Amino Acid Sequence Animals Antigens, Bacterial - administration & dosage Antigens, Bacterial - genetics Antigens, Bacterial - isolation & purification Bacteriology Base Sequence Biological and medical sciences BOVIN BRUCELLA ABORTUS Brucella abortus - chemistry Brucella abortus - genetics Brucella abortus - immunology Cattle Chromatography, Ion Exchange CLONACION MOLECULAR CLONAGE MOLECULAIRE Cloning, Molecular COMPOSICION QUIMICA COMPOSITION CHIMIQUE Cross Reactions Cytoplasm - immunology Female Fundamental and applied biological sciences. Psychology GANADO BOVINO Genes, Bacterial Hybridomas - chemistry Hybridomas - immunology Hypersensitivity, Delayed - etiology Immunodominant Epitopes - administration & dosage Immunodominant Epitopes - genetics Immunodominant Epitopes - isolation & purification Lymphocyte Activation Microbiology Molecular Sequence Data Molecular Weight Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains SECUENCIA NUCLEOTIDICA SEQUENCE NUCLEOTIDIQUE Bovine Nucleotide sequence Host agent relation Delayed hypersensitivity Cellular immunity Brucella abortus Immunodominance Infection Proteins Vertebrata Mammalia Brucellaceae Animal Bacteriosis Bacteria Brucellosis Artiodactyla Veterinary Molecular cloning Ungulata Cytoplasm Aminoacid sequence Structural analysis
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Summary:	Monoclonal antibodies and polyclonal antisera recognizing a 39-kDa protein (P39) of brucellin, a cytoplasmic extract from Brucella melitensis rough strain B115, were produced. The P39 was purified by anion-exchange chromatography. Eleven of fourteen Brucella-infected cows whose infections had been detected by the delayed-type hypersensitivity (DTH) test with brucellergen also developed a DTH reaction when purified P39 was used as the trigger. The T-cell proliferative responses to P39 of peripheral blood lymphocytes from Brucella-infected cows were also positive. None of the animals infected with other bacterial species that are presumed to induce immunological cross-reactions with Brucella spp. reacted to P39, either in DTH tests or in lymphocyte proliferation assays. A lambda gt11 genomic library of Brucella abortus was screened with a monoclonal antibody specific for P39, and the gene coding for this protein was subsequently isolated. The nucleotide sequence of the P39 gene was determined, and the deduced amino acid sequence is in accordance with the sequence of an internal peptide isolated from P39
Bibliography:	L73 9735987 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0019-9567 1098-5522
DOI:	10.1128/iai.65.2.495-502.1997