Use of ferrets for electrophysiologic monitoring of ion transport

Limited success achieved in translating basic science discoveries into clinical applications for chronic airway diseases is attributed to differences in respiratory anatomy and physiology, poor approximation of pathologic processes, and lack of correlative clinical endpoints between humans and labor...

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Bibliographic Details
Published in:	PloS one Vol. 12; no. 10; p. e0186984
Main Authors:	Kaza, Niroop, Raju, S Vamsee, Cadillac, Joan M, Trombley, John A, Rasmussen, Lawrence, Tang, Liping, Dohm, Erik, Harrod, Kevin S, Rowe, Steven M
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 27-10-2017 Public Library of Science (PLoS)
Subjects:	Anatomy Anesthesiology Animal experimentation Animal models Animals Biology Biology and Life Sciences Bronchi - cytology Bronchi - metabolism Bronchi - physiology Bronchitis Cell culture Chronic obstructive pulmonary disease Cystic fibrosis Cystic Fibrosis Transmembrane Conductance Regulator - metabolism Electrophysiologic tests Electrophysiological Phenomena Epithelial cells Epithelial Cells - metabolism Epithelial Cells - physiology Epithelial Sodium Channels - metabolism Epithelium Explants Feasibility studies Ferrets Ferrets - physiology In vitro methods and tests Ion Transport Laboratory animals Lung diseases Mammals Medicine Medicine and Health Sciences Methods Mustela putorius furo Physical Sciences Physiological aspects Physiology Respiratory tract diseases Studies Technology application Therapeutic applications Trachea - cytology Trachea - metabolism Trachea - physiology Alabama United States > US
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Summary:	Limited success achieved in translating basic science discoveries into clinical applications for chronic airway diseases is attributed to differences in respiratory anatomy and physiology, poor approximation of pathologic processes, and lack of correlative clinical endpoints between humans and laboratory animal models. Here, we discuss advantages of using ferrets (Mustela putorus furo) as a model for improved understanding of human airway physiology and demonstrate assays for quantifying airway epithelial ion transport in vivo and ex vivo, and establish air-liquid interface cultures of ferret airway epithelial cells as a complementary in vitro model for mechanistic studies. We present data here that establishes the feasibility of measuring these human disease endpoints in ferrets. Briefly, potential difference across the nasal and the lower airway epithelium in ferrets could be consistently assessed, were highly reproducible, and responsive to experimental interventions. Additionally, ferret airway epithelial cells were amenable to primary cell culture methods for in vitro experiments as was the use of ferret tracheal explants as an ex vivo system for assessing ion transport. The feasibility of conducting multiple assessments of disease outcomes supports the adoption of ferrets as a highly relevant model for research in obstructive airway diseases.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Competing Interests: The laboratory is funded by Astra Zeneca, on projects unrelated to the present work. This does not alter our adherence to PloS One policies on sharing data and materials.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0186984