Early Induction of Angiotensin I-Converting Enzyme in Rat Carotid Artery After Balloon Injury

Several studies have demonstrated the effectiveness of angiotensin I-converting enzyme (ACE) inhibitors in preventing the neointima formation found after denudation of the rat carotid artery by balloon injury. The aim of the present study was to determine the role of ACE in this model and to compare...

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Bibliographic Details
Published in:	Hypertension (Dallas, Tex. 1979) Vol. 30; no. 2; pp. 272 - 277
Main Authors:	Fernandez-Alfonso, Maria S, Martorana, Piero A, Licka, Ivo, van Even, Paul, Trobisch, Dieter, Scholkens, Bernward A, Paul, Martin
Format:	Journal Article
Language:	English
Published:	Philadelphia, PA American Heart Association, Inc 01-08-1997 Hagerstown, MD Lippincott
Subjects:	Angiotensin-Converting Enzyme Inhibitors - pharmacology Animals Arterial Occlusive Diseases - prevention & control Biological and medical sciences Biphenyl Compounds - pharmacology Cardiovascular system Carotid Arteries - enzymology Carotid Arteries - pathology Carotid Artery Injuries Catheterization DNA - metabolism Enzyme Induction Imidazoles - pharmacology Male Medical sciences Peptidyl-Dipeptidase A - genetics Peptidyl-Dipeptidase A - metabolism Pharmacology. Drug treatments Polymerase Chain Reaction Ramipril - analogs & derivatives Ramipril - pharmacology Rats Rats, Sprague-Dawley RNA, Messenger - metabolism Tunica Intima - drug effects Tunica Intima - pathology Vascular wall Wounds, Nonpenetrating - enzymology Wounds, Nonpenetrating - pathology Rat Enzyme Rodentia Instrumentation therapy Cuffed tube Cardiovascular disease Instrumental dilatation Trauma Vascular disease Prevention Peptidases Vertebrata Chemotherapy Mammalia Treatment Peptidyl-dipeptidase A Animal Carotid Hydrolases Peptidyl-dipeptidases Complication Ramiprilat Comparative study ACE inhibitor
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Summary:	Several studies have demonstrated the effectiveness of angiotensin I-converting enzyme (ACE) inhibitors in preventing the neointima formation found after denudation of the rat carotid artery by balloon injury. The aim of the present study was to determine the role of ACE in this model and to compare the treatment with the ACE inhibitor ramiprilat with that with the angiotensin II antagonist HR 720. The endothelial layer of the left carotid artery was removed using an inflated balloon catheter. Injured and control vessels were both submitted to histomorphological analysis and DNA content quantification at 2, 4, 6, 8, 12, and 14 days after injury. Evaluation of neointima thickening demonstrated a slow but steady increase of neointima that was significant after day 6 and reached 30% of the lumen in 2 weeks. This was paralleled by an increase in DNA content, which was significant 4 days after injury. ACE mRNA levels were quantified by polymerase chain reaction after reverse transcription. Measurement of ACE mRNA levels revealed a significant upregulation 2 and 8 days after injury, with no significant difference when compared with control tissue at later time points. ACE activity was also significantly enhanced at 2 and 8 days after injury, with no significant difference when compared with control tissue at later time points. In addition, the treatment with ramiprilat was more efficient in reducing neointima formation than that with HR 720. These data underlie the role of ACE in this model of restenosis. The early induction of ACE expression after endothelial injury but before significant changes in the vessel structure suggests that ACE activity might be one of the mechanisms that trigger neointima formation in the rat. (Hypertension. 1997;30[part 1]:272-277.)
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0194-911X 1524-4563
DOI:	10.1161/01.hyp.30.2.272