Self-antibacterial chitosan/Aloe barbadensis Miller hydrogels releasing nitrite for biomedical applications

[Display omitted] Because of the toxic glutaraldehyde, the chitosan-glutaraldehyde hydrogels are seriously limited in biomedical applications. In this study, exploiting bioactive compounds of Aloe barbadensis Miller, the chitosan/Aloe barbadensis Miller-glutaraldehyde (CS/AV-GDA) hydrogels were fabr...

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Bibliographic Details
Published in:	Journal of industrial and engineering chemistry (Seoul, Korea) Vol. 103; pp. 175 - 186
Main Authors:	Hoang Thi, Thai Thanh, Trinh, Binh D.T., Le Thi, Phuong, Tran, Dieu Linh, Park, Ki Dong, Nguyen, Dai Hai
Format:	Journal Article
Language:	English
Published:	Elsevier B.V 25-11-2021 한국공업화학회
Subjects:	Aloe barbadensis Miller Antibacterial hydrogels Chitosan Nitrite Wound dressing 화학공학 Antibacterial hydrogels Aloe barbadensis Miller Wound dressing Chitosan Nitrite
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Summary:	[Display omitted] Because of the toxic glutaraldehyde, the chitosan-glutaraldehyde hydrogels are seriously limited in biomedical applications. In this study, exploiting bioactive compounds of Aloe barbadensis Miller, the chitosan/Aloe barbadensis Miller-glutaraldehyde (CS/AV-GDA) hydrogels were fabricated with 4-fold lower glutaraldehyde concentration without compromising hydrogel characteristics. The gelation time was controlled from a few seconds to several hours. The elastic modulus was varied from 483 to 99940 Pa. The CS/AV-GDA hydrogels could release the natural nitrite amount from 24.0 to 89.6 μM within the first hour for antibacterial activity, then continuously deliver a few μM every next hour for cell activities. The antibacterial test against Escherichia coli and Staphylococcus aureus revealed that the CS/AV-GDA hydrogels could kill the planktonic bacteria 5-fold more highly than control and prevent bacteria attachment on hydrogel surface effectively. Although the CS/AV-GDA hydrogels consumed only 0.25% glutaraldehyde concentration, their antibacterial capacities were comparable to chitosan-only hydrogels with 2% glutaraldehyde. For cytotoxicity tests, the CS/AV-GDA hydrogels using 0.25% glutaraldehyde concentration induce the human dermal fibroblasts proliferation significantly. All CS/AV-GDA hydrogels with glutaraldehyde crosslinker less than 1% showed non-cytotoxicity. As a result, the new CS/AV-GDA hydrogels might become an attractive candidate for medicine regeneration and tissue engineering.
ISSN:	1226-086X 1876-794X
DOI:	10.1016/j.jiec.2021.07.029