Nasal vaccination with attenuated Salmonella expressing VapA: TLR2 activation is not essential for protection against R. equi infection

Highlights • Administration of attenuated Salmonella harbouring the VapA antigen (STM VapA+) via nasal route induced a protective immune response. • STM VapA+ protects TLR2-KO and TLR4-deficient mice against R. equi challenge. • STM VapA+ stimulates antigen-specific CD4+ T cells without the particip...

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Bibliographic Details
Published in:	Vaccine Vol. 31; no. 41; pp. 4528 - 4535
Main Authors:	Cardoso, Silvia Almeida, Oliveira, Aline Ferreira, Ruas, Luciana Pereira, Trevisani, Marcel Montels, De Oliveira, Leandro Licursi, Hanna, Ebert Seixas, Roque-Barreira, Maria Cristina, Soares, Sandro Gomes
Format:	Journal Article
Language:	English
Published:	Kidlington Elsevier Ltd 23-09-2013 Elsevier Elsevier Limited
Subjects:	Actinomycetales Infections - immunology Actinomycetales Infections - prevention & control Administration, Intranasal Allergy and Immunology Animals Antigens Applied microbiology Attenuated Salmonella Attenuation Bacterial Load Bacterial Proteins - genetics Bacterial Proteins - immunology Bacterial Vaccines - administration & dosage Bacterial Vaccines - genetics Bacterial Vaccines - immunology Bacteriology Biological and medical sciences CD4 antigen CD4-positive T-lymphocytes Cell-mediated immunity Drug Carriers Experiments Female Flow cytometry Fundamental and applied biological sciences. Psychology Genetic Vectors Humoral immunity Hydrogen peroxide Immune clearance Immune response Immune system Immunity Immunization Immunologic Memory Immunological memory Infections interferon-gamma interleukin-12 lipoproteins Lymphocytes Lymphocytes T Macrophages Memory cells Mice Mice, Inbred BALB C Mice, Inbred C3H Mice, Inbred C57BL Mice, Knockout microbial load Microbiology Miscellaneous Nitric oxide nose phenotype Phenotypes plasmids Proteins Rhodococcus equi Rhodococcus equi - genetics Rhodococcus equi - immunology Salmonella Salmonella enterica typhimurium Salmonella Typhimurium Salmonella typhimurium - genetics Spleen Spleen - immunology Spleen - microbiology splenocytes Statistical analysis Studies T-Lymphocytes - immunology TLR2 protein TLR4 protein Toll-like receptor 2 Toll-Like Receptor 2 - deficiency Toll-Like Receptor 2 - immunology Toll-Like Receptor 4 - deficiency Toll-Like Receptor 4 - immunology Toll-like receptors vaccination Vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) VapA Vectored vaccine Virulence Virulence factors virulent strains γ-Interferon United States > US Toll-like receptors Vectored vaccine Rhodococcus equi VapA Attenuated Salmonella Salmonella Vaccination Toll like receptor Vaccine Bacteria Actinomycetes Veterinary Vector Enterobacteriaceae
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Summary:	Highlights • Administration of attenuated Salmonella harbouring the VapA antigen (STM VapA+) via nasal route induced a protective immune response. • STM VapA+ protects TLR2-KO and TLR4-deficient mice against R. equi challenge. • STM VapA+ stimulates antigen-specific CD4+ T cells without the participation of TLR2. • TLR2 is required for immune response recall in a mouse model.
Bibliography:	http://dx.doi.org/10.1016/j.vaccine.2013.07.067 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2013.07.067