Spatial and temporal MRI profile of ischemic tissue after the acute stages of a permanent mouse model of stroke

To characterize the progression of injured tissue resulting from a permanent focal cerebral ischemia after the acute phase, Magnetic Resonance Imaging (MRI) monitoring was performed on adult male C57BL/6J mice in the subacute stages, and correlated to histological analyses. Lesions were induced by e...

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Bibliographic Details
Published in:	The open neuroimaging journal Vol. 7; pp. 4 - 14
Main Authors:	Bogaert-Buchmann, A, Poittevin, M, Po, C, Dupont, D, Sebrié, C, Tomita, Y, Trandinh, A, Seylaz, J, Pinard, E, Méric, P, Kubis, N, Gillet, B
Format:	Journal Article
Language:	English
Published:	United Arab Emirates Bentham Open 01-02-2013
Subjects:	permanent cerebral ischemia Mouse microglia stroke NMR Imaging
Online Access:	Get full text
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Summary:	To characterize the progression of injured tissue resulting from a permanent focal cerebral ischemia after the acute phase, Magnetic Resonance Imaging (MRI) monitoring was performed on adult male C57BL/6J mice in the subacute stages, and correlated to histological analyses. Lesions were induced by electrocoagulation of the middle cerebral artery. Serial MRI measurements and weighted-images (T2, T1, T2* and Diffusion Tensor Imaging) were performed on a 9.4T scanner. Histological data (Cresyl-Violet staining and laminin-, Iba1- and GFAP-immunostainings) were obtained 1 and 2 weeks after the stroke. Two days after stroke, tissues assumed to correspond to the infarct core, were detected as a hyperintensity signal area in T2-weighted images. One week later, low-intensity signal areas appeared. Longitudinal MRI study showed that these areas remained present over the following week, and was mainly linked to a drop of the T2 relaxation time value in the corresponding tissues. Correlation with histological data and immuno-histochemistry showed that these areas corresponded to microglial cells. The present data provide, for the first time detailed MRI parameters of microglial cells dynamics, allowing its non-invasive monitoring during the chronic stages of a stroke. This could be particularly interesting in regards to emerging anti-inflammatory stroke therapies.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1874-4400 1874-4400
DOI:	10.2174/1874440001307010004