Production and Quantification of Virulence Factors in Malassezia Species

Production and Quantification of Virulence Factors in Malassezia Species

Seventy-seven strains of were included in this study. Biofilm and hydrolytic enzyme production were studied by using specific solid media. The Real-Time reverse transcriptase qPCR method was applied to determine the overexpression of genes encoding the extracellular enzymes. All included species pro...

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Bibliographic Details
Published in:Polish journal of microbiology Vol. 71; no. 4; pp. 529 - 538
Main Authors: Hadrich, Inès, Khemekhem, Nahed, Neji, Sourour, Trablesi, Houaida, Ilahi, Amin, Sellami, Hayet, Makni, Fattouma, Ayadi, Ali
Format: Journal Article
Language:English
Published: Poland De Gruyter Poland 06-12-2022
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Subjects:
biofilm
Biofilms
Enzymatic activity
Enzymes
Extracellular enzymes
extracellular hydrolases
Folliculitis
Gene expression
Genes
Humans
Keratinase
Lipase
Lipase - metabolism
Malassezia
Malassezia - genetics
Original Paper
Pityriasis
Pityriasis versicolor
real-time qrt-pcr
RNA-directed DNA polymerase
Species
Statistical analysis
Tinea Versicolor
Virulence
Virulence Factors
Malassezia
extracellular hydrolases
Real-Time qRT-PCR
virulence factors
biofilm
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Summary:Seventy-seven strains of were included in this study. Biofilm and hydrolytic enzyme production were studied by using specific solid media. The Real-Time reverse transcriptase qPCR method was applied to determine the overexpression of genes encoding the extracellular enzymes. All included species produced biofilms. No statistically significant difference was observed between species in biofilm formation ( = 0.567). All species produced lipase, and 95% of showed a strong enzymatic activity (Pz = 0.55 ± 0.02). A statistically significant difference was observed between the mean keratinase indices of and the other species ( = 0.005). The overexpression of one or more genes was observed in 100% of strains isolated from patients with folliculitis, 87.5% - with pityriasis versicolor, and 57.14% of the control group isolates. A statistically significant difference in the lipase gene expression ( = 0.042) was between the strains from patients with folliculitis and the control group. This investigation provides more information about the frequency of the production of the major enzymes considered virulence factors of species. Interestingly, the overexpression of one or more genes was observed in strains isolated from patients with disorders.
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ISSN:2544-4646
1733-1331
2544-4646
DOI:10.33073/pjm-2022-047