An Assessment of the AdenoPlus Point-of-Care Test for Diagnosing Adenoviral Conjunctivitis and Its Effect on Antibiotic Stewardship

To determine the sensitivity and specificity of the AdenoPlus test compared with real-time polymerase chain reaction (PCR) and to determine whether there was a reduction in antibiotic prescriptions with the use of AdenoPlus compared with the previous year. A total of 125 patients with suspected infe...

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Bibliographic Details
Published in:	Mayo Clinic proceedings. Innovations, quality & outcomes Vol. 1; no. 2; pp. 170 - 175
Main Authors:	Holtz, Kelly K., Townsend, Kalie R., Furst, Joseph W., Myers, Jane F., Binnicker, Matthew J., Quigg, Stephanie M., Maxson, Julie A., Espy, Mark J.
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier Inc 01-09-2017 Elsevier
Subjects:	Original POC PCR HIPAA PCR, polymerase chain reaction HIPAA, Health Insurance Portability and Accountability Act POC, point-of-care
Online Access:	Get full text
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Summary:	To determine the sensitivity and specificity of the AdenoPlus test compared with real-time polymerase chain reaction (PCR) and to determine whether there was a reduction in antibiotic prescriptions with the use of AdenoPlus compared with the previous year. A total of 125 patients with suspected infectious conjunctivitis were accrued from June 4, 2015, through September 27, 2015. Forty-six participants from the prospective cohort completed both AdenoPlus and PCR testing. Two hundred fifty age-matched individuals were in the retrospective cohort. There was a significant reduction in the percentage of patients who received an antibiotic ophthalmic prescription in the prospective cohort vs the retrospective cohort (32% vs 45%; χ2P=.01). AdenoPlus test sensitivity was 50% (5 of 10) and specificity was 92% (33 of 36) compared with real-time PCR testing. The AdenoPlus test has high specificity for diagnosing adenoviral conjunctivitis but lower sensitivity than has been previously published. These data suggest that negative AdenoPlus results should be confirmed by real-time PCR owing to the low overall sensitivity of AdenoPlus observed.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2542-4548 2542-4548
DOI:	10.1016/j.mayocpiqo.2017.06.001