CT promoter element binding protein: definition of a double-strand and a novel single-strand DNA binding motif

Numerous genes contain promoter elements that are nuclease hypersensitive. These elements frequently possess polypurine/polypyrimidine stretches and are usually associated with altered chromatin structure. We have previously isolated a clone that binds a class of CT-rich promoter elements. We have f...

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Bibliographic Details
Published in:	Nucleic acids research Vol. 20; no. 1; pp. 111 - 116
Main Authors:	Kolluri, R, Torrey, T.A, Kinniburgh, A.J
Format:	Journal Article
Language:	English
Published:	Oxford Oxford University Press 11-01-1992
Subjects:	Base Sequence binding sites Biological and medical sciences DNA - genetics DNA - metabolism DNA conformation DNA Mutational Analysis DNA, Single-Stranded - genetics DNA, Single-Stranded - metabolism DNA-binding proteins DNA-Binding Proteins - metabolism Fundamental and applied biological sciences. Psychology Molecular and cellular biology Molecular genetics Molecular Sequence Data nucleases oligopeptides Promoter Regions, Genetic - genetics Transcription. Transcription factor. Splicing. Rna processing Chromatin Enzyme Transcription promoter DNA binding protein Molecular interaction Single stranded DNA Secondary structure Vertebrata Double stranded DNA Sensitivity Mammalia Structure activity relation CT rich sequence Deletion Mutation Nuclease
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Summary:	Numerous genes contain promoter elements that are nuclease hypersensitive. These elements frequently possess polypurine/polypyrimidine stretches and are usually associated with altered chromatin structure. We have previously isolated a clone that binds a class of CT-rich promoter elements. We have further characterized this clone, termed the nuclease-sensitive element protein-1, or NSEP-1. NSEP-1 binds both duplex CT elements and the CT-rich strand of these elements in a 'generic' sequence specific manner and has overlapping but distinct single- and double-strand DNA binding domains. The minimal peptide region sufficient for both duplex and single-strand DNA binding includes two regions rich in basic amino acids flanking an RNP-CS-1 like octapeptide motif. Deletion analysis shows that the single-strand DNA binding activity is mediated by the RNP-CS-1 like octapeptide motif and is the key peptide region necessary for single-strand binding. NSEP-1's affinity for CT rich promoter elements with strand asymmetry in addition to its double- and single-strand DNA binding properties suggests that it may be a member of a class of DNA binding proteins that modulate gene expression by their ability to recognize DNA with unusual secondary structure.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0305-1048 1362-4962
DOI:	10.1093/nar/20.1.111