NtWBC1, an ABC transporter gene specifically expressed in tobacco reproductive organs

To identify genes specifically or predominantly expressed in the stigmas/styles and to establish their possible function in the reproductive process of plants, a tobacco stigma/style cDNA library was constructed and differentially screened, resulting in the isolation of several cDNA clones. The mole...

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Bibliographic Details
Published in:	Journal of experimental botany Vol. 55; no. 403; pp. 1643 - 1654
Main Authors:	Otsu, C.T, daSilva, I, Molfetta, J.B. de, Silva, L.R. da, Almeida-Engler, J. de, Engler, G, Torraca, P.C, Goldman, G.H, Goldman, M.H.S
Format:	Journal Article
Language:	English
Published:	Oxford Oxford University Press 01-08-2004 Oxford Publishing Limited (England)
Subjects:	ABC transporter ABC transporters Agronomy. Soil science and plant productions Amino Acid Sequence amino acid sequences Anthers ATP binding cassette transporters ATP-Binding Cassette Transporters - genetics Biological and medical sciences Classical and quantitative genetics. Population genetics. Molecular genetics Cloning, Molecular Complementary DNA developmentally regulated DNA flowering Flowers - genetics Flowers - growth & development Flowers - metabolism Fundamental and applied biological sciences. Psychology Gene Expression Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Gene Library Generalities. Genetics. Plant material Genes Genetics and breeding of economic plants Genome, Plant Genomes Genomics in situ hybridization model organisms Molecular genetics Molecular Sequence Data Nicotiana - genetics Nicotiana - metabolism Nicotiana tabacum nucleotide sequences Plant Proteins - genetics plant tissues Proteins Reproduction Research Papers: Cell and Molecular Biology, Biochemistry and Molecular Physiology RNA Sequence Alignment stamen expression Stamens stigma stigma-predominant expression stigmatic secretory zone styles tobacco White subfamily Anther Seeds developmentally regulated stamen expression Stamen Root Genomics stigma-predominant expression Plant leaf Hybridization Nicotiana tabacum Stem Complementary DNA Gene Dicotyledones stigmatic secretory zone Angiospermae Spermatophyta Solanaceae ATP ABC transporter White subfamily
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Summary:	To identify genes specifically or predominantly expressed in the stigmas/styles and to establish their possible function in the reproductive process of plants, a tobacco stigma/style cDNA library was constructed and differentially screened, resulting in the isolation of several cDNA clones. The molecular characterization of one of these clones is described here. After sequencing the cDNA and the isolated genomic clone, it was determined that the corresponding gene encodes a protein containing an ATP-binding cassette, characteristic of ABC transporters. This gene, designated as NtWBC1 (Nicotiana tabacum ABC transporter of the White-Brown Complex subfamily), encodes a protein that contains the typical structure of the 'half-transporters' of the White subfamily. To establish the spatial expression pattern of the NtWBC1 gene, northern blot and real-time RT-PCR analyses with total RNA from roots, stems, leaves, sepals, petals, stamens, stigmas/styles, ovaries, and seeds were performed. The result revealed a transcript of 2.5 kb present at high levels in stigmas and styles and a smaller transcript (2.3 kb) present at a lower level in stamens. NtWBC1 expression is developmentally regulated in stigmas/styles, with mRNA accumulation increasing toward anthesis. In situ hybridization experiments demonstrated that NtWBC1 is expressed in the stigmatic secretory zone and in anthers, at the stomium region and at the vascular bundle. NtWBC1 is the first ABC transporter gene with specific expression in plant reproductive organs to be identified and its expression pattern suggests important role(s) in the reproductive process.
Bibliography:	istex:603BF6B025215FC475D69AA3BDB27F7606948735 local:erh195 To whom correspondence should be addressed. Fax: +55 16 633 1758. E-mail: mgoldman@ffclrp.usp.br ark:/67375/HXZ-DQSS8JM5-F ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0022-0957 1460-2431 1460-2431
DOI:	10.1093/jxb/erh195