An ABC transporter involved in the control of streptomycin production in Streptomyces griseus

We screened for a gene that inhibits streptomycin production in Streptomyces griseus when it is introduced on a high-copy-number plasmid pIJ702, and obtained a plasmid pKM545. The introduction of pKM545 abolished streptomycin production on all media tested including YMP-sugar and Nutrient broth. S1...

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Bibliographic Details
Published in:	FEMS microbiology letters Vol. 363; no. 14; p. fnw149
Main Authors:	Takano, Hideaki, Toriumi, Naoe, Hirata, Mariko, Amano, Taisuke, Ohya, Takaaki, Shimada, Reona, Kusada, Hiroyuki, Amano, Sho-ichi, Matsuda, Ko-ichi, Beppu, Teruhiko, Ueda, Kenji
Format:	Journal Article
Language:	English
Published:	England Oxford University Press 01-07-2016
Subjects:	Amino Acid Sequence ATP-Binding Cassette Transporters - genetics ATP-Binding Cassette Transporters - metabolism Bacterial Proteins - genetics Bacterial Proteins - metabolism Biosynthesis Cloning, Molecular Drug therapy Gene Order Genes Genetic Loci Microbiology Mutation Plasmids - genetics Protein Interaction Domains and Motifs Streptomyces griseus - genetics Streptomyces griseus - metabolism Streptomycin - biosynthesis ABC transporter streptomycin Streptomyces griseus
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Summary:	We screened for a gene that inhibits streptomycin production in Streptomyces griseus when it is introduced on a high-copy-number plasmid pIJ702, and obtained a plasmid pKM545. The introduction of pKM545 abolished streptomycin production on all media tested including YMP-sugar and Nutrient broth. S1 protection analysis demonstrated that the introduction of this plasmid downregulated the transcriptional activity of the promoter preceding strR, the pathway-specific transcriptional regulator for streptomycin biosynthesis. The 2.8-kb BamHI fragment cloned onto pKM545 contained two coding sequences SGR_5442 and 5443. These coding sequences and the two downstream ones (SGR_5444 and 5445) constituted a possible operon structure designated to be rspABCD (regulation of streptomycin production). RspB and RspC exhibited a marked similarity with an ATP-binding domain and a membrane-associating domain of an ABC-2 type transporter, respectively, suggesting that the Rsp proteins comprise a membrane exporter. The gene cluster consisting of the rsp operon and the upstream divergent small coding sequence (SGR_5441) was widely distributed to Streptomyces genome. An rspB mutant of S. griseus produced 3-fold streptomycin of the parental strain in YMP liquid medium. The evidence implies that the Rsp translocator is involved in the export of a substance that specifies the expression level of streptomycin biosynthesis genes in S. griseus. An ABC transporter involved in the control of streptomycin production in Streptomyces griseus.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1574-6968 0378-1097 1574-6968
DOI:	10.1093/femsle/fnw149