Search Results - "Torchia, Dennis A."

Probing exchange kinetics and atomic resolution dynamics in high-molecular-weight complexes using dark-state exchange saturation transfer NMR spectroscopy by Fawzi, Nicolas L, Ying, Jinfa, Torchia, Dennis A, Clore, G Marius

“…We present the protocol for the measurement and analysis of dark-state exchange saturation transfer (DEST), a novel solution NMR method for characterizing, at…”
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Simultaneous NMR Study of Protein Structure and Dynamics Using Conservative Mutagenesis by Yao, Lishan, Vögeli, Beat, Torchia, Dennis A, Bax, Ad

“…A novel iterative procedure is described that allows both the orientation and dynamics of internuclear bond vectors to be determined from direct interpretation…”
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Sparse multidimensional iterative lineshape-enhanced (SMILE) reconstruction of both non-uniformly sampled and conventional NMR data by Ying, Jinfa, Delaglio, Frank, Torchia, Dennis A., Bax, Ad

“…Implementation of a new algorithm, SMILE, is described for reconstruction of non-uniformly sampled two-, three- and four-dimensional NMR data, which takes…”
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Extending the range of amide proton relaxation dispersion experiments in proteins using a constant-time relaxation-compensated CPMG approach by Ishima, Rieko, Torchia, Dennis A

“…Relaxation compensated constant-time Carr-Purcell-Meiboom-Gill relaxation dispersion experiments for amide protons are presented that detect mus-ms time-scale…”
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Mutational and Structural Studies Aimed at Characterizing the Monomer of HIV-1 Protease and Its Precursor by Ishima, Rieko, Torchia, Dennis A., Louis, John M.

“…An experimental protocol for folding the mature human immunodeficiency virus-1 (HIV-1) protease is presented that facilitates NMR studies at a low protein…”
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NMR studies of dynamic biomolecular conformational ensembles by Torchia, Dennis A

“…Multidimensional heteronuclear NMR approaches can provide nearly complete sequential signal assignments of isotopically enriched biomolecules. The availability…”
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Advances in NMR Spectroscopy of Weakly Aligned Biomolecular Systems by Chiliveri, Sai Chaitanya, Robertson, Angus J., Shen, Yang, Torchia, Dennis A., Bax, Ad

“…The measurement and application of residual dipolar couplings (RDCs) in solution NMR studies of biological macromolecules has become well established over the…”
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Atomic-resolution dynamics on the surface of amyloid-β protofibrils probed by solution NMR by Fawzi, Nicolas L., Ying, Jinfa, Ghirlando, Rodolfo, Torchia, Dennis A., Clore, G. Marius

“…Visibility cloak for hidden proteins When proteins associate with larger structures such as polymers, membranes or solid supports, they usually become…”
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An NMR Experiment for the Accurate Measurement of Heteronuclear Spin-Lock Relaxation Rates by Korzhnev, Dmitry M, Skrynnikov, Nikolai R, Millet, Oscar, Torchia, Dennis A, Kay, Lewis E

“…Rotating-frame relaxation rates, R 1 ρ, are often measured in NMR studies of protein dynamics. We show here that large systematic errors can be introduced into…”
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Remarkable Rigidity of the Single α‑Helical Domain of Myosin-VI As Revealed by NMR Spectroscopy by Barnes, C. Ashley, Shen, Yang, Ying, Jinfa, Takagi, Yasuharu, Torchia, Dennis A, Sellers, James R, Bax, Ad

“…Although the α-helix has long been recognized as an all-important element of secondary structure, it generally requires stabilization by tertiary interactions…”
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Kinetics of Amyloid β Monomer-to-Oligomer Exchange by NMR Relaxation by Fawzi, Nicolas L, Ying, Jinfa, Torchia, Dennis A, Clore, G. Marius

“…Recent studies have implicated non-fibrillar oligomers of the amyloid β (Aβ) peptide as the primary toxic species in Alzheimer’s disease. Detailed structural…”
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Error estimation and global fitting in transverse-relaxation dispersion experiments to determine chemical-exchange parameters by Ishima, Rieko, Torchia, Dennis A

“…Off-resonance effects can introduce significant systematic errors in R2 measurements in constant-time Carr-Purcell-Meiboom-Gill (CPMG) transverse relaxation…”
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A solution NMR study of the binding kinetics and the internal dynamics of an HIV‐1 protease‐substrate complex by Katoh, Etsuko, Louis, John M., Yamazaki, Toshimasa, Gronenborn, Angela M., Torchia, Dennis A., Ishima, Rieko

“…NMR studies of the binding of a substrate to an inactive HIV‐1 protease construct, containing an active site mutation PRD25N, are reported. Substrate titration…”
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Sparse multidimensional iterative lineshape-enhanced (SMILE) reconstruction of both non-uniformly sampled and conventional NMR data by Ying, Jinfa, Delaglio, Frank, Torchia, Dennis A., Bax, Ad

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Estimating the time scale of chemical exchange of proteins from measurements of transverse relaxation rates in solution by Ishima, R, Torchia, D A

“…Chemical (conformational) exchange on the ms--microsecond time scale is reliably identified by the observation of transverse relaxation rates, Rex, that depend…”
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Dynamics of biomolecules from picoseconds to seconds at atomic resolution by Torchia, Dennis A.

“…Although biomolecular dynamics has been investigated using NMR for at least 40 years, only in the past 20 years have internal motions been characterized at…”
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Protein dynamics from NMR by Ishima, Rieko, Torchia, Dennis A

“…This review surveys recent investigations of conformational fluctuations of proteins in solution using NMR techniques. Advances in experimental methods have…”
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ARTSY-J: Convenient and precise measurement of (3)JHNHα couplings in medium-size proteins from TROSY-HSQC spectra by Roche, Julien, Ying, Jinfa, Shen, Yang, Torchia, Dennis A, Bax, Ad

“…A new and convenient method, named ARTSY-J, is introduced that permits extraction of the (3)JHNHα couplings in proteins from the relative intensities in a pair…”
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Measuring rapid hydrogen exchange in the homodimeric 36 kDa HIV‐1 integrase catalytic core domain by Fitzkee, Nicholas C., Torchia, Dennis A., Bax, Ad

“…Measurements of rapid hydrogen exchange (HX) of water with protein amide sites contain valuable information on protein structure and function, but current NMR…”
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Monitoring Hydrogen Exchange During Protein Folding by Fast Pressure Jump NMR Spectroscopy by Alderson, T. Reid, Charlier, Cyril, Torchia, Dennis A, Anfinrud, Philip, Bax, Ad

“…A method is introduced that permits direct observation of the rates at which backbone amide hydrogens become protected from solvent exchange after rapidly…”
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