Search Results - "Toonen, Ruud F."

Munc13-1 and Munc18-1 together prevent NSF-dependent de-priming of synaptic vesicles by He, Enqi, Wierda, Keimpe, van Westen, Rhode, Broeke, Jurjen H., Toonen, Ruud F., Cornelisse, L. Niels, Verhage, Matthijs

“…Synaptic transmission requires a stable pool of release-ready (primed) vesicles. Here we show that two molecules involved in SNARE-complex assembly, Munc13-1…”
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Mapping localization of 21 endogenous proteins in the Golgi apparatus of rodent neurons by van Bommel, Danique M., Toonen, Ruud F., Verhage, Matthijs

“…The Golgi apparatus is the major sorting hub in the secretory pathway and particularly important for protein sorting in neurons. Knowledge about protein…”
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Vti1a/b support distinct aspects of TGN and cis-/medial Golgi organization by van Bommel, Danique M., Toonen, Ruud F., Verhage, Matthijs

“…Retrograde trafficking towards the trans -Golgi network (TGN) is important for dense core vesicle (DCV) biogenesis. Here, we used Vti1a/b deficient neurons to…”
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The RAB3-RIM Pathway Is Essential for the Release of Neuromodulators by Persoon, Claudia M., Hoogstraaten, Rein I., Nassal, Joris P., van Weering, Jan R.T., Kaeser, Pascal S., Toonen, Ruud F., Verhage, Matthijs

“…Secretion principles are conserved from yeast to humans, and many yeast orthologs have established roles in synaptic vesicle exocytosis in the mammalian brain…”
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CaMKII controls neuromodulation via neuropeptide gene expression and axonal targeting of neuropeptide vesicles by Moro, Alessandro, van Woerden, Geeske M, Toonen, Ruud F, Verhage, Matthijs

“…Ca2+/calmodulin-dependent kinase II (CaMKII) regulates synaptic plasticity in multiple ways, supposedly including the secretion of neuromodulators like…”
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Protein instability, haploinsufficiency, and cortical hyper-excitability underlie STXBP1 encephalopathy by Kovačević, Jovana, Maroteaux, Gregoire, Schut, Desiree, Loos, Maarten, Dubey, Mohit, Pitsch, Julika, Remmelink, Esther, Koopmans, Bastijn, Crowley, James, Cornelisse, L Niels, Sullivan, Patrick F, Schoch, Susanne, Toonen, Ruud F, Stiedl, Oliver, Verhage, Matthijs

“…De novo heterozygous mutations in STXBP1 cause early infantile epileptic encephalopathy. Kovačević et al. analyse the underlying disease mechanisms in silico,…”
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Early Golgi Abnormalities and Neurodegeneration upon Loss of Presynaptic Proteins Munc18-1, Syntaxin-1, or SNAP-25 by Santos, Tatiana C, Wierda, Keimpe, Broeke, Jurjen H, Toonen, Ruud F, Verhage, Matthijs

“…The loss of presynaptic proteins Munc18-1, syntaxin-1, or SNAP-25 is known to produce cell death, but the underlying features have not been compared…”
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Vti1a/b regulate synaptic vesicle and dense core vesicle secretion via protein sorting at the Golgi by Emperador-Melero, Javier, Huson, Vincent, van Weering, Jan, Bollmann, Christian, Fischer von Mollard, Gabriele, Toonen, Ruud F., Verhage, Matthijs

“…The SNAREs Vti1a/1b are implicated in regulated secretion, but their role relative to canonical exocytic SNAREs remains elusive. Here, we show that synaptic…”
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Munc18-1 in secretion: lonely Munc joins SNARE team and takes control by Toonen, Ruud F.G, Verhage, Matthijs

“…SNARE proteins and the Sec1/Munc18 (SM) protein, Munc18-1, are essential components of the mammalian secretion machinery. Until recently, quite divergent…”
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A Single-Cell Model for Synaptic Transmission and Plasticity in Human iPSC-Derived Neurons by Meijer, Marieke, Rehbach, Kristina, Brunner, Jessie W., Classen, Jessica A., Lammertse, Hanna C.A., van Linge, Lola A., Schut, Desiree, Krutenko, Tamara, Hebisch, Matthias, Cornelisse, L. Niels, Sullivan, Patrick F., Peitz, Michael, Toonen, Ruud F., Brüstle, Oliver, Verhage, Matthijs

“…Synaptic dysfunction is associated with many brain disorders, but robust human cell models to study synaptic transmission and plasticity are lacking. Instead,…”
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Liprin-α2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission by Spangler, Samantha A, Schmitz, Sabine K, Kevenaar, Josta T, de Graaff, Esther, de Wit, Heidi, Demmers, Jeroen, Toonen, Ruud F, Hoogenraad, Casper C

“…The presynaptic active zone mediates synaptic vesicle exocytosis, and modulation of its molecular composition is important for many types of synaptic…”
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Differential axonal trafficking of Neuropeptide Y-, LAMP1-, and RAB7-tagged organelles in vivo by Nassal, Joris P, Murphy, Fiona H, Toonen, Ruud F, Verhage, Matthijs

“…Different organelles traveling through neurons exhibit distinct properties in vitro, but this has not been investigated in the intact mammalian brain. We…”
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Phosphorylation of synaptotagmin-1 controls a post-priming step in PKC-dependent presynaptic plasticity by de Jong, Arthur P. H., Meijer, Marieke, Saarloos, Ingrid, Cornelisse, Lennart Niels, Toonen, Ruud F. G., Sørensen, Jakob B., Verhage, Matthijs

“…Presynaptic activation of the diacylglycerol (DAG)/protein kinase C (PKC) pathway is a central event in short-term synaptic plasticity. Two substrates,…”
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Rabphilin-3A negatively regulates neuropeptide release, through its SNAP25 interaction by Abramian, Adlin, Hoogstraaten, Rein I, Murphy, Fiona H, McDaniel, Kathryn F, Toonen, Ruud F, Verhage, Matthijs

“…Neuropeptides and neurotrophins are stored in and released from dense core vesicles (DCVs). While DCVs and synaptic vesicles (SVs) share fundamental SNARE/SM…”
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Munc13 controls the location and efficiency of dense-core vesicle release in neurons by van de Bospoort, Rhea, Farina, Margherita, Schmitz, Sabine K, de Jong, Arthur, de Wit, Heidi, Verhage, Matthijs, Toonen, Ruud F

“…Neuronal dense-core vesicles (DCVs) contain diverse cargo crucial for brain development and function, but the mechanisms that control their release are largely…”
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Multi-level characterization of balanced inhibitory-excitatory cortical neuron network derived from human pluripotent stem cells by Nadadhur, Aishwarya G, Emperador Melero, Javier, Meijer, Marieke, Schut, Desiree, Jacobs, Gerbren, Li, Ka Wan, Hjorth, J J Johannes, Meredith, Rhiannon M, Toonen, Ruud F, Van Kesteren, Ronald E, Smit, August B, Verhage, Matthijs, Heine, Vivi M

“…Generation of neuronal cultures from induced pluripotent stem cells (hiPSCs) serve the studies of human brain disorders. However we lack neuronal networks with…”
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Automated analysis of neuronal morphology, synapse number and synaptic recruitment by Schmitz, Sabine K., Hjorth, J.J. Johannes, Joemai, Raoul M.S., Wijntjes, Rick, Eijgenraam, Susanne, de Bruijn, Petra, Georgiou, Christina, de Jong, Arthur P.H., van Ooyen, Arjen, Verhage, Matthijs, Cornelisse, L. Niels, Toonen, Ruud F., Veldkamp, Wouter

“…[Display omitted] ▶ SynD reliably analyzes dendrite length and synapse number. ▶ SynD automatically quantifies dendritic branching and synaptic localization. ▶…”
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Tomosyn affects dense core vesicle composition but not exocytosis in mammalian neurons by Subkhangulova, Aygul, Gonzalez-Lozano, Miguel A, Groffen, Alexander JA, van Weering, Jan RT, Smit, August B, Toonen, Ruud F, Verhage, Matthijs

“…Tomosyn is a large, non-canonical SNARE protein proposed to act as an inhibitor of SNARE complex formation in the exocytosis of secretory vesicles. In the…”
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Synaptic Effects of Munc18-1 Alternative Splicing in Excitatory Hippocampal Neurons by Meijer, Marieke, Cijsouw, Tony, Toonen, Ruud F, Verhage, Matthijs

“…The munc18-1 gene encodes two splice-variants that vary at the C-terminus of the protein and are expressed at different levels in different regions of the…”
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Munc18-1 is a dynamically regulated PKC target during short-term enhancement of transmitter release by Genc, Ozgür, Kochubey, Olexiy, Toonen, Ruud F, Verhage, Matthijs, Schneggenburger, Ralf

“…Transmitter release at synapses is regulated by preceding neuronal activity, which can give rise to short-term enhancement of release like post-tetanic…”
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