Search Results - "Toogood, Helen S."

Biocatalytic Reductions and Chemical Versatility of the Old Yellow Enzyme Family of Flavoprotein Oxidoreductases by Toogood, Helen S., Gardiner, John M., Scrutton, Nigel S.

“…The old yellow enzyme (OYE) family is a large group of flavin‐dependent redox biocatalysts with major applications in the industrial reduction of activated…”
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From Bugs to Bioplastics: Total (+)‐Dihydrocarvide Biosynthesis by Engineered Escherichia coli by Ascue Avalos, Gabriel A., Toogood, Helen S., Tait, Shirley, Messiha, Hanan L., Scrutton, Nigel S.

“…The monoterpenoid lactone derivative (+)‐dihydrocarvide ((+)‐DHCD) can be polymerised to form shape‐memory polymers. Synthetic biology routes from simple,…”
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Consolidated Bioprocessing: Synthetic Biology Routes to Fuels and Fine Chemicals by Banner, Alec, Toogood, Helen S., Scrutton, Nigel S.

“…The long road from emerging biotechnologies to commercial “green” biosynthetic routes for chemical production relies in part on efficient microbial use of…”
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Dynamics driving function - new insights from electron transferring flavoproteins and partner complexes by Toogood, Helen S, Leys, David, Scrutton, Nigel S

“…Electron transferring flavoproteins (ETFs) are soluble heterodimeric FAD-containing proteins that function primarily as soluble electron carriers between…”
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Co‐production of biofuel, bioplastics and biochemicals during extended fermentation of Halomonas bluephagenesis by Park, Helen, Toogood, Helen S., Chen, Guo‐Qiang, Scrutton, Nigel S.

“…Halomonas bluephagenesis TD1.0 was engineered to produce the biofuel propane, bioplastic poly‐3‐hydroxybutyrate (PHB), and biochemicals mandelate and…”
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Chemoenzymatic Synthesis of the Intermediates in the Peppermint Monoterpenoid Biosynthetic Pathway by Cheallaigh, Aisling Ní, Mansell, David J, Toogood, Helen S, Tait, Shirley, Lygidakis, Antonios, Scrutton, Nigel S, Gardiner, John M

“…A chemoenzymatic approach providing access to all four intermediates in the peppermint biosynthetic pathway between limonene and menthone/isomenthone,…”
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Bioproduction of Linalool From Paper Mill Waste by Rinaldi, Mauro A., Tait, Shirley, Toogood, Helen S., Scrutton, Nigel S.

“…A key challenge in chemicals biomanufacturing is the maintenance of stable, highly productive microbial strains to enable cost-effective fermentation at scale…”
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Discovery, Characterization, Engineering, and Applications of Ene-Reductases for Industrial Biocatalysis by Toogood, Helen S, Scrutton, Nigel S

“…Recent studies of multiple enzyme families collectively called ene-reductases (ERs) have highlighted potential industrial applications of these biocatalysts in…”
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Biocatalytic Asymmetric Alkene Reduction: Crystal Structure and Characterization of a Double Bond Reductase from Nicotiana tabacum by Mansell, David J, Toogood, Helen S, Waller, John, Hughes, John M. X, Levy, Colin W, Gardiner, John M, Scrutton, Nigel S

“…The application of biocatalysis for the asymmetric reduction of activated CC is a powerful tool for the manufacture of high-value chemical commodities. The…”
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Chemoautotrophic production of gaseous hydrocarbons, bioplastics and osmolytes by a novel Halomonas species by Faulkner, Matthew, Hoeven, Robin, Kelly, Paul P, Sun, Yaqi, Park, Helen, Liu, Lu-Ning, Toogood, Helen S, Scrutton, Nigel S

“…Abstract Background Production of relatively low value, bulk commodity chemicals and fuels by microbial species requires a step-change in approach to decrease…”
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Structure-Based Insight into the Asymmetric Bioreduction of the CC Double Bond of α,β-Unsaturated Nitroalkenes by Pentaerythritol Tetranitrate Reductase by Toogood, Helen S., Fryszkowska, Anna, Hare, Victoria, Fisher, Karl, Roujeinikova, Anna, Leys, David, Gardiner, John M., Stephens, Gill M., Scrutton, Nigel S.

“…Biocatalytic reduction of α‐ or β‐alkyl‐β‐arylnitroalkenes provides a convenient and efficient method to prepare chiral substituted nitroalkanes…”
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Focused Directed Evolution of Pentaerythritol Tetranitrate Reductase by Using Automated Anaerobic Kinetic Screening of Site‐Saturated Libraries by Hulley, Martyn E, Toogood, Helen S, Fryszkowska, Anna, Mansell, David, Stephens, Gill M, Gardiner, John M, Scrutton, Nigel S

“…This work describes the development of an automated robotic platform for the rapid screening of enzyme variants generated from directed evolution studies of…”
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New developments in ‘ene’-reductase catalysed biological hydrogenations by Toogood, Helen S, Scrutton, Nigel S

“…•Old Yellow Enzymes are primarily used for biocatalytic hydrogenations.•Medium chain dehydrogenase/reductases can substitute for OYEs.•Unsaturated ester…”
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Mechanism of Coenzyme Binding to Human Methionine Synthase Reductase Revealed through the Crystal Structure of the FNR-like Module and Isothermal Titration Calorimetry by Wolthers, Kirsten R, Lou, Xiaodong, Toogood, Helen S, Leys, David, Scrutton, Nigel S

“…Human methionine synthase reductase (MSR) is a 78 kDa flavoprotein that regenerates the active form of cobalamin-dependent methionine synthase (MS). MSR…”
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Combinatorial use of environmental stresses and genetic engineering to increase ethanol titres in cyanobacteria by Andrews, Fraser, Faulkner, Matthew, Toogood, Helen S, Scrutton, Nigel S

“…Current industrial bioethanol production by yeast through fermentation generates carbon dioxide. Carbon neutral bioethanol production by cyanobacteria uses…”
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Retooling microorganisms for the fermentative production of alcohols by Toogood, Helen S, Scrutton, Nigel S

“…[Display omitted] •Synthetic biology engineering technology applied to introduce alcohol biofuel-producing pathways into microorganisms.•‘Biofactories’ fed on…”
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Stabilization of Non-productive Conformations Underpins Rapid Electron Transfer to Electron-transferring Flavoprotein by Toogood, Helen S., van Thiel, Adam, Scrutton, Nigel S., Leys, David

“…Crystal structures of protein complexes with electron-transferring flavoprotein (ETF) have revealed a dual protein-protein interface with one region serving as…”
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Renewable and tuneable bio-LPG blends derived from amino acids by Amer, Mohamed, Hoeven, Robin, Kelly, Paul, Faulkner, Matthew, Smith, Michael H, Toogood, Helen S, Scrutton, Nigel S

“…Microbial biorefinery approaches are beginning to define renewable and sustainable routes to clean-burning and non-fossil fuel-derived gaseous alkanes (known…”
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Biocatalytic Routes to Lactone Monomers for Polymer Production by Messiha, Hanan L, Ahmed, Syed T, Karuppiah, Vijaykumar, Suardíaz, Reynier, Ascue Avalos, Gabriel A, Fey, Natalie, Yeates, Stephen, Toogood, Helen S, Mulholland, Adrian J, Scrutton, Nigel S

“…Monoterpenoids offer potential as biocatalytically derived monomer feedstocks for high-performance renewable polymers. We describe a biocatalytic route to…”
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Alternative Hydride Sources for Ene-Reductases: Current Trends by Toogood, Helen S., Knaus, Tanja, Scrutton, Nigel S.

“…Hydride source: Commercially viable biocatalytic reductions must overcome the cost barrier of NAD(P)H requirements. Developments in overcoming the “hydride…”
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