Search Results - "Tong, Yaojun"

A versatile genetic engineering toolkit for E. coli based on CRISPR-prime editing by Tong, Yaojun, Jørgensen, Tue S., Whitford, Christopher M., Weber, Tilmann, Lee, Sang Yup

“…CRISPR base editing is a powerful method to engineer bacterial genomes. However, it restricts editing to single-nucleotide substitutions. Here, to address this…”
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CRISPR–Cas9, CRISPRi and CRISPR-BEST-mediated genetic manipulation in streptomycetes by Tong, Yaojun, Whitford, Christopher M., Blin, Kai, Jørgensen, Tue S., Weber, Tilmann, Lee, Sang Yup

“…Streptomycetes are prominent sources of bioactive natural products, but metabolic engineering of the natural products of these organisms is greatly hindered by…”
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Natural products research in the modern age by Tong, Yaojun

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Dissemination of antibiotic resistance genes from antibiotic producers to pathogens by Jiang, Xinglin, Ellabaan, Mostafa M. Hashim, Charusanti, Pep, Munck, Christian, Blin, Kai, Tong, Yaojun, Weber, Tilmann, Sommer, Morten O. A., Lee, Sang Yup

“…It has been hypothesized that some antibiotic resistance genes (ARGs) found in pathogenic bacteria derive from antibiotic-producing actinobacteria. Here we…”
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Discovering the next decade's synthetic biology research trends with ChatGPT by Tong, Yaojun, Zhang, Lixin

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A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules by Liang, Mindong, Li, Zilong, Wang, Weishan, Liu, Jiakun, Liu, Leshi, Zhu, Guoliang, Karthik, Loganathan, Wang, Man, Wang, Ke-Feng, Wang, Zhong, Yu, Jing, Shuai, Yuting, Yu, Jiaming, Zhang, Lu, Yang, Zhiheng, Li, Chuan, Zhang, Qian, Shi, Tong, Zhou, Liming, Xie, Feng, Dai, Huanqin, Liu, Xueting, Zhang, Jingyu, Liu, Guang, Zhuo, Ying, Zhang, Buchang, Liu, Chenli, Li, Shanshan, Xia, Xuekui, Tong, Yaojun, Liu, Yanwen, Alterovitz, Gil, Tan, Gao-Yi, Zhang, Li-Xin

“…Besides genome editing, CRISPR-Cas12a has recently been used for DNA detection applications with attomolar sensitivity but, to our knowledge, it has not been…”
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Revolutionizing biotechnology advances natural products discovery and industrial processing by Tong, Yaojun, Weber, Tilmann

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An aurora of natural products-based drug discovery is coming by Tong, Yaojun, Deng, Zixin

“…Natural products (NPs), a nature's reservoir possessing enormous structural and functional diversity far beyond the current ability of chemical synthesis, are…”
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Designing sgRNAs for CRISPR-BEST base editing applications with CRISPy-web 2.0 by Blin, Kai, Shaw, Simon, Tong, Yaojun, Weber, Tilmann

“…CRISPR/Cas9 systems are an established tool in genome engineering. As double strand breaks caused by the standard Cas9-based knock-out techniques can be…”
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Roles of Candida albicans Gat2, a GATA-type zinc finger transcription factor, in biofilm formation, filamentous growth and virulence by Du, Han, Guan, Guobo, Xie, Jing, Sun, Yuan, Tong, Yaojun, Zhang, Lixin, Huang, Guanghua

“…Candida albicans is the most common human fungal pathogen, causing not only superficial infections, but also life-threatening systemic disease. C. albicans can…”
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White-opaque switching in natural MTLa/α isolates of Candida albicans: evolutionary implications for roles in host adaptation, pathogenesis, and sex by Xie, Jing, Tao, Li, Nobile, Clarissa J, Tong, Yaojun, Guan, Guobo, Sun, Yuan, Cao, Chengjun, Hernday, Aaron D, Johnson, Alexander D, Zhang, Lixin, Bai, Feng-Yan, Huang, Guanghua

“…Phenotypic transitions play critical roles in host adaptation, virulence, and sexual reproduction in pathogenic fungi. A minority of natural isolates of…”
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Synthetic Biology Facilitates Antimicrobials Discovery by Bai, Linquan, Deng, Zixin, Tong, Yaojun

“…We are currently facing two big global challenges: antibiotics shortage and multidrug resistance [...]…”
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Integrating PCR-free amplification and synergistic sensing for ultrasensitive and rapid CRISPR/Cas12a-based SARS-CoV-2 antigen detection by Zhao, Xiangxiang, Wang, Zhengduo, Yang, Bowen, Li, Zilong, Tong, Yaojun, Bi, Yuhai, Li, Zhenghong, Xia, Xuekui, Chen, Xiangyin, Zhang, Lixin, Wang, Weishan, Tan, Gao-Yi

“…Antigen detection provides particularly valuable information for medical diagnoses; however, the current detection methods are less sensitive and accurate than…”
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Trichodermaketones A−D and 7-O-Methylkoninginin D from the Marine Fungus Trichoderma koningii by Song, Fuhang, Dai, Huanqin, Tong, Yaojun, Ren, Biao, Chen, Caixia, Sun, Nuo, Liu, Xiangyang, Bian, Jiang, Liu, Mei, Gao, Hong, Liu, Hongwei, Chen, Xiaoping, Zhang, Lixin

“…Five new polyketide derivatives, 7-O-methylkoninginin D (1) and trichodermaketones A−D (2−5), together with four known compounds, koninginins A, D, E, and F,…”
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N-Acetylglucosamine Induces White-to-Opaque Switching and Mating in Candida tropicalis, Providing New Insights into Adaptation and Fungal Sexual Evolution by Xie, Jing, Du, Han, Guan, Guobo, Tong, Yaojun, Kourkoumpetis, Themistoklis K, Zhang, Lixin, Bai, Feng-yan, Huang, Guanghua

“…Pathogenic fungi are capable of switching between different phenotypes, each of which has a different biological advantage. In the most prevalent human fungal…”
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An in vitro CRISPR-Cas12a-mediated protocol for direct cloning of large DNA fragments by Zeng, Xiaoqian, Wang, Shuliu, Liang, Mindong, Wang, Weishan, Jiang, Yue, Xu, Fei, Liu, Leshi, Yan, Hao, Tong, Yaojun, Zhang, Lixin, Tan, Gao-Yi

“…Large biosynthetic gene cluster (BGC) cloning is important for discovering natural product-based drugs and remains challenging in high GC content…”
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Programmable polyketide biosynthesis platform for production of aromatic compounds in yeast by Jakočiūnas, Tadas, Klitgaard, Andreas K., Kontou, Eftychia Eva, Nielsen, Julie Bang, Thomsen, Emil, Romero-Suarez, David, Blin, Kai, Petzold, Christopher J., Gin, Jennifer W., Tong, Yaojun, Gotfredsen, Charlotte Held, Charusanti, Pep, Frandsen, Rasmus J.N., Weber, Tilmann, Lee, Sang Yup, Jensen, Michael K., Keasling, Jay D.

“…To accelerate the shift to bio-based production and overcome complicated functional implementation of natural and artificial biosynthetic pathways to industry…”
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Beauvericin counteracted multi-drug resistant Candida albicans by blocking ABC transporters by Tong, Yaojun, Liu, Mei, Zhang, Yu, Liu, Xueting, Huang, Ren, Song, Fuhang, Dai, Huanqin, Ren, Biao, Sun, Nuo, Pei, Gang, Bian, Jiang, Jia, Xin-Ming, Huang, Guanghua, Zhou, Xuyu, Li, Shaojie, Zhang, Buchang, Fukuda, Takashi, Tomoda, Hiroshi, Ōmura, Satoshi, Cannon, Richard D., Calderone, Richard, Zhang, Lixin

“…Multi-drug resistance of pathogenic microorganisms is becoming a serious threat, particularly to immunocompromised populations. The high mortality of…”
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Optogenetic tools for microbial synthetic biology by Chia, Natalie, Lee, Sang Yup, Tong, Yaojun

“…Chemical induction is one of the most common modalities used to manipulate gene expression in living systems. However, chemical induction can be toxic or…”
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Synthetic biology and metabolic engineering of actinomycetes for natural product discovery by Palazzotto, Emilia, Tong, Yaojun, Lee, Sang Yup, Weber, Tilmann

“…Actinomycetes are one of the most valuable sources of natural products with industrial and medicinal importance. After more than half a century of…”
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