Genetic Variability Determinants of Helicobacter pylori: Influence of Clinical Background and Geographic Origin of Isolates

Helicobacter pylori has an unusual pattern of genetic variation, which complicates research on this organism. To gain a better understanding of the forces behind this phenomenon, the extent to which recombination and single point mutations affect genetic variability in H. pylori was quantified and t...

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Bibliographic Details
Published in:	The Journal of infectious diseases Vol. 181; no. 5; pp. 1674 - 1681
Main Authors:	Göttke, Markus U., Fallone, Carlo A., Barkun, Alan N., Vogt, Konstanze, Loo, Vivian, Trautmann, Matthias, Tong, Jian Z., Nguyen, Thanh N., Fainsilber, Toby, Hahn, Helmut H., Körber, Jürgen, Lowe, Aviva, Beech, Robin N.
Format:	Journal Article
Language:	English
Published:	Chicago, IL The University of Chicago Press 01-05-2000 University of Chicago Press Oxford University Press
Subjects:	Alleles Bacterial Proteins - genetics Bacteriology Berlin Biological and medical sciences DNA Flagellin - genetics Fundamental and applied biological sciences. Psychology Gastric Mucosa - microbiology Genetic diversity Genetic equilibrium Genetic Variation Geography Helicobacter pylori Helicobacter pylori - classification Helicobacter pylori - genetics Helicobacter pylori - isolation & purification Humans Major Articles Microbiology Nucleotides Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Phylogeny Point mutation Polymerase Chain Reaction Quebec Restriction Mapping Species diversity Berlin Quebec Canada Germany Human Genetic variability Spirillales Helicobacter pylori Phylogenetic tree Spirillaceae Bacteria Genotype Clinical isolate
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Summary:	Helicobacter pylori has an unusual pattern of genetic variation, which complicates research on this organism. To gain a better understanding of the forces behind this phenomenon, the extent to which recombination and single point mutations affect genetic variability in H. pylori was quantified and the influence of both geographical distance and clinical background were assessed. Site-directed restriction-endonuclease digestion of 2 gene fragments was performed on 168 isolates from Montreal and Berlin. Allelic diversity was found to be much higher for H. pylori than for other bacterial species. This finding is consistent with those of previous studies on H. pylori that were conducted using other techniques. However, nucleotide diversity was within the range reported for other bacterial species. Phylogenetic analysis found no grouping of strains with clinical background or geographical origin. Recombination at a rate that resulted in linkage equilibrium within genes can explain these observations.
Bibliography:	istex:6810037037E56660496F2594BB390EF3CAE87B47 ark:/67375/HXZ-66B0RV91-L ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0022-1899 1537-6613
DOI:	10.1086/315425