Reclassification of the Specialized Metabolite Producer Pseudomonas mesoacidophila ATCC 31433 as a Member of the Burkholderia cepacia Complex

ATCC 31433 is a Gram-negative bacterium, first isolated from Japanese soil samples, that produces the monobactam isosulfazecin and the β-lactam-potentiating bulgecins. To characterize the biosynthetic potential of ATCC 31433, its complete genome was determined using single-molecule real-time DNA seq...

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Bibliographic Details
Published in:	Journal of bacteriology Vol. 199; no. 13
Main Authors:	Loveridge, E Joel, Jones, Cerith, Bull, Matthew J, Moody, Suzy C, Kahl, Małgorzata W, Khan, Zainab, Neilson, Louis, Tomeva, Marina, Adams, Sarah E, Wood, Andrew C, Rodriguez-Martin, Daniel, Pinel, Ingrid, Parkhill, Julian, Mahenthiralingam, Eshwar, Crosby, John
Format:	Journal Article
Language:	English
Published:	United States American Society for Microbiology 01-07-2017
Subjects:	Anti-Bacterial Agents - pharmacology Antibiotic resistance Antibiotics Bacteriology Biosynthesis Burkholderia cepacia Burkholderia cepacia complex - classification Burkholderia cepacia complex - drug effects Burkholderia cepacia complex - genetics Deoxyribonucleic acid DNA DNA, Bacterial - genetics Drug Resistance, Bacterial Gene clusters Gene Expression Regulation, Bacterial - physiology Genome, Bacterial - genetics Genomes Gram-negative bacteria Metabolites Nucleotide sequence Peptides Phylogeny Polyketides Pseudomonas Pseudomonas - classification Pseudomonas - drug effects Pseudomonas - genetics Reclassification rRNA Sulfazecin genome metal resistance bulgecin identification antibiotic resistance biosynthesis antibacterial
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Summary:	ATCC 31433 is a Gram-negative bacterium, first isolated from Japanese soil samples, that produces the monobactam isosulfazecin and the β-lactam-potentiating bulgecins. To characterize the biosynthetic potential of ATCC 31433, its complete genome was determined using single-molecule real-time DNA sequence analysis. The 7.8-Mb genome comprised four replicons, three chromosomal (each encoding rRNA) and one plasmid. Phylogenetic analysis demonstrated that ATCC 31433 was misclassified at the time of its deposition and is a member of the complex, most closely related to The sequenced genome shows considerable additional biosynthetic potential; known gene clusters for malleilactone, ornibactin, isosulfazecin, alkylhydroxyquinoline, and pyrrolnitrin biosynthesis and several uncharacterized biosynthetic gene clusters for polyketides, nonribosomal peptides, and other metabolites were identified. Furthermore, ATCC 31433 harbors many genes associated with environmental resilience and antibiotic resistance and was resistant to a range of antibiotics and metal ions. In summary, this bioactive strain should be designated complex strain ATCC 31433, pending further detailed taxonomic characterization. This work reports the complete genome sequence of ATCC 31433, a known producer of bioactive compounds. Large numbers of both known and novel biosynthetic gene clusters were identified, indicating that ATCC 31433 is an untapped resource for discovery of novel bioactive compounds. Phylogenetic analysis demonstrated that ATCC 31433 is in fact a member of the complex, most closely related to the species Further investigation of the classification and biosynthetic potential of ATCC 31433 is warranted.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Present address: Sarah E. Adams, The Francis Crick Institute, London, United Kingdom; Daniel Rodriguez-Martin, Centro de Investigación en Sanidad Animal, Madrid, Spain; Ingrid Pinel, Faculty of Applied Sciences, Delft University, Delft, Netherlands. Citation Loveridge EJ, Jones C, Bull MJ, Moody SC, Kahl MW, Khan Z, Neilson L, Tomeva M, Adams SE, Wood AC, Rodriguez-Martin D, Pinel I, Parkhill J, Mahenthiralingam E, Crosby J. 2017. Reclassification of the specialized metabolite producer Pseudomonas mesoacidophila ATCC 31433 as a member of the Burkholderia cepacia complex. J Bacteriol 199:e00125-17. https://doi.org/10.1128/JB.00125-17.
ISSN:	0021-9193 1098-5530
DOI:	10.1128/JB.00125-17