The single laser flow cytometric micronucleus test: a time course study using colchicine and urethane in rat and mouse peripheral blood and acetaldehyde in rat peripheral blood

A single laser flow cytometric procedure to quantify micronucleus frequency in rat and mouse peripheral blood was evaluated. Reticulocytes express the transferrin receptor (also known as the CD71-defined antigen). When combined with a DNA stain, antibodies against this antigen can be used to differe...

Full description

Saved in:

Bibliographic Details
Published in:	Mutagenesis Vol. 17; no. 1; pp. 15 - 23
Main Authors:	Hynes, G.M., Torous, D.K., Tometsko, C.R., Burlinson, B., Gatehouse, D.G.
Format:	Journal Article
Language:	English
Published:	Oxford Oxford University Press 01-01-2002
Subjects:	Acetaldehyde - toxicity Animals Biological and medical sciences Blood Cell Count - instrumentation Blood Cell Count - methods Blood Cells - drug effects Bone Marrow Cells - drug effects Carcinogens - toxicity Colchicine - toxicity Cyclophosphamide - pharmacology Flow Cytometry - instrumentation Flow Cytometry - methods Fluorescein-5-isothiocyanate - analysis Fluorescent Dyes - analysis General aspects. Methods Lasers Male Medical sciences Mice Micronucleus Tests - methods Organ Specificity Rats Rats, Wistar Receptors, Transferrin - blood Reproducibility of Results Reticulocytes - chemistry Reticulocytes - drug effects Toxicology Urethane - toxicity Chromosomal aberration Flow cytometry Micronucleus test Rat Mutagenicity testing Toxicity Multicenter study Rodentia Method Urethane Acetaldehyde Vertebrata Mammalia Mouse Reticulocyte Colchicine
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	A single laser flow cytometric procedure to quantify micronucleus frequency in rat and mouse peripheral blood was evaluated. Reticulocytes express the transferrin receptor (also known as the CD71-defined antigen). When combined with a DNA stain, antibodies against this antigen can be used to differentially label and quantify micronucleated reticulocytes. The object of this study was to evaluate the method for rat and mouse peripheral blood using flow cytometry and compare the results obtained between two laboratories (GlaxoWellcome and Litron Laboratories). The compounds selected were the rodent carcinogens colchicine, urethane and acetaldehyde. Colchicine gives a positive response in the rat bone marrow micronucleus assay and an inconclusive result in the rat peripheral blood micronucleus assay. The latter two are both established rat carcinogens readily detected in both the bone marrow and peripheral blood micronucleus assays. In these experiments both rat and mice were treated with either colchicine or urethane and rats alone treated with acetaldehyde. After a single treatment, repeat sampling of peripheral blood was made at 0, 24, 48 and 72 h. Replicate blood samples were obtained and fixed for flow cytometric analysis at both facilities. The micronucleated reticulocyte frequency of each blood sample was determined by analysing 20 000 total reticulocytes per blood sample. The data suggest that the single laser flow cytometric procedure resulted in consistent reticulocyte and micronucleated reticulocyte frequencies between laboratories. Furthermore, these flow cytometric data compare favourably with previously published data.
Bibliography:	local:0170015 ark:/67375/HXZ-R2DHHGMJ-1 PII:1464-3804 istex:2C8A06CA4B5112A28E5D05339437027EC3487515 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0267-8357 1464-3804 1464-3804
DOI:	10.1093/mutage/17.1.15