Search Results - "Tomabechi, Yuri"

Chiral deaza-coelenterazine analogs for probing a substrate-binding site in the Ca2+-binding photoprotein aequorin by Inouye, Satoshi, Sumida, Yuto, Tomabechi, Yuri, Taguchi, Jumpei, Shirouzu, Mikako, Hosoya, Takamitsu

“…The Ca2+-binding photoprotein aequorin is a complex of apoAequorin (apoprotein) and (S)-2-peroxycoelenterazine. Aequorin can be regenerated by the incubation…”
Get full text

Reverse mutants of the catalytic 19 kDa mutant protein (nanoKAZ/nanoLuc) from Oplophorus luciferase with coelenterazine as preferred substrate by Inouye, Satoshi, Sato, Jun-ichi, Sahara-Miura, Yuiko, Tomabechi, Yuri, Sumida, Yuto, Sekine, Shun-ichi, Shirouzu, Mikako, Hosoya, Takamitsu

“…Native Oplophorus luciferase (OpLase) and its catalytic 19 kDa protein (wild KAZ) show highest luminescence activity with coelenterazine (CTZ) among CTZ…”
Get full text

CAMSAP2 organizes a γ-tubulin-independent microtubule nucleation centre through phase separation by Imasaki, Tsuyoshi, Kikkawa, Satoshi, Niwa, Shinsuke, Saijo-Hamano, Yumiko, Shigematsu, Hideki, Aoyama, Kazuhiro, Mitsuoka, Kaoru, Shimizu, Takahiro, Aoki, Mari, Sakamoto, Ayako, Tomabechi, Yuri, Sakai, Naoki, Shirouzu, Mikako, Taguchi, Shinya, Yamagishi, Yosuke, Setsu, Tomiyoshi, Sakihama, Yoshiaki, Nitta, Eriko, Takeichi, Masatoshi, Nitta, Ryo

“…Microtubules are dynamic polymers consisting of αβ-tubulin heterodimers. The initial polymerization process, called microtubule nucleation, occurs…”
Get full text

Amyloid conformation-dependent disaggregation in a reconstituted yeast prion system by Nakagawa, Yoshiko, Shen, Howard C.-H., Komi, Yusuke, Sugiyama, Shinju, Kurinomaru, Takaaki, Tomabechi, Yuri, Krayukhina, Elena, Okamoto, Kenji, Yokoyama, Takeshi, Shirouzu, Mikako, Uchiyama, Susumu, Inaba, Megumi, Niwa, Tatsuya, Sako, Yasushi, Taguchi, Hideki, Tanaka, Motomasa

“…Disaggregation of amyloid fibrils is a fundamental biological process required for amyloid propagation. However, due to the lack of experimental systems, the…”
Get full text

Lysosome-associated membrane proteins-1 and -2 (LAMP-1 and LAMP-2) assemble via distinct modes by Terasawa, Kazue, Tomabechi, Yuri, Ikeda, Mariko, Ehara, Haruhiko, Kukimoto-Niino, Mutsuko, Wakiyama, Motoaki, Podyma-Inoue, Katarzyna A., Rajapakshe, Anupama R., Watabe, Tetsuro, Shirouzu, Mikako, Hara-Yokoyama, Miki

“…Lysosome-associated membrane proteins 1 and 2 (LAMP-1 and LAMP-2) have a large, heavily glycosylated luminal domain composed of two subdomains, and are the…”
Get full text

Chemical reversal of abnormalities in cells carrying mitochondrial DNA mutations by Kobayashi, Hiroki, Hatakeyama, Hideyuki, Nishimura, Haruna, Yokota, Mutsumi, Suzuki, Sadafumi, Tomabechi, Yuri, Shirouzu, Mikako, Osada, Hiroyuki, Mimaki, Masakazu, Goto, Yu-ichi, Yoshida, Minoru

“…Mitochondrial DNA (mtDNA) mutations are the major cause of mitochondrial diseases. Cells harboring disease-related mtDNA mutations exhibit various phenotypic…”
Get full text

Slow luminescence kinetics of semi-synthetic aequorin: expression, purification and structure determination of cf3-aequorin by Inouye, Satoshi, Tomabechi, Yuri, Hosoya, Takamitsu, Sekine, Shun-ichi, Shirouzu, Mikako

“…Abstract cf3-Aequorin is one of the semi-synthetic aequorins that was produced by replacing 2-peroxycoelenterazine (CTZ-OOH) in native aequorin with a…”
Get full text

Crystal Structure of an Archaeal Tyrosyl-tRNA Synthetase Bound to Photocaged L-Tyrosine and Its Potential Application to Time-Resolved X-ray Crystallography by Hosaka, Toshiaki, Katsura, Kazushige, Ishizuka-Katsura, Yoshiko, Hanada, Kazuharu, Ito, Kaori, Tomabechi, Yuri, Inoue, Mio, Akasaka, Ryogo, Takemoto, Chie, Shirouzu, Mikako

“…Genetically encoded caged amino acids can be used to control the dynamics of protein activities and cellular localization in response to external cues. In the…”
Get full text

Phosphorylated and non-phosphorylated HCK kinase domains produced by cell-free protein expression by Katsura, Kazushige, Tomabechi, Yuri, Matsuda, Takayoshi, Yonemochi, Mayumi, Mikuni, Junko, Ohsawa, Noboru, Terada, Takaho, Yokoyama, Shigeyuki, Kukimoto-Niino, Mutsuko, Takemoto, Chie, Shirouzu, Mikako

“…Since phosphorylation is involved in various physiological events, kinases and interacting factors can be potential targets for drug discovery. For the…”
Get full text

Protein stabilization utilizing a redefined codon by Ohtake, Kazumasa, Yamaguchi, Atsushi, Mukai, Takahito, Kashimura, Hiroki, Hirano, Nobutaka, Haruki, Mitsuru, Kohashi, Sosuke, Yamagishi, Kenji, Murayama, Kazutaka, Tomabechi, Yuri, Itagaki, Takashi, Akasaka, Ryogo, Kawazoe, Masahito, Takemoto, Chie, Shirouzu, Mikako, Yokoyama, Shigeyuki, Sakamoto, Kensaku

“…Recent advances have fundamentally changed the ways in which synthetic amino acids are incorporated into proteins, enabling their efficient and multiple-site…”
Get full text

A reproducible and scalable procedure for preparing bacterial extracts for cell-free protein synthesis by Katsura, Kazushige, Matsuda, Takayoshi, Tomabechi, Yuri, Yonemochi, Mayumi, Hanada, Kazuharu, Ohsawa, Noboru, Sakamoto, Kensaku, Takemoto, Chie, Shirouzu, Mikako

Get full text

Potent SARS-CoV-2 neutralizing antibodies with therapeutic effects in two animal models by Takeshita, Masaru, Fukuyama, Hidehiro, Kamada, Katsuhiko, Matsumoto, Takehisa, Makino-Okamura, Chieko, Uchikubo-Kamo, Tomomi, Tomabechi, Yuri, Hanada, Kazuharu, Moriyama, Saya, Takahashi, Yoshimasa, Ishigaki, Hirohito, Nakayama, Misako, Nguyen, Cong Thanh, Kitagawa, Yoshinori, Itoh, Yasushi, Imai, Masaki, Maemura, Tadashi, Furusawa, Yuri, Ueki, Hiroshi, Iwatsuki-Horimoto, Kiyoko, Ito, Mutsumi, Yamayoshi, Seiya, Kawaoka, Yoshihiro, Shirouzu, Mikako, Ishii, Makoto, Saya, Hideyuki, Kondo, Yasushi, Kaneko, Yuko, Suzuki, Katsuya, Fukunaga, Koichi, Takeuchi, Tsutomu

“…The use of therapeutic neutralizing antibodies against SARS-CoV-2 infection has been highly effective. However, there remain few practical antibodies against…”
Get full text

Potent neutralizing broad-spectrum antibody against SARS-CoV-2 generated from dual-antigen-specific B cells from convalescents by Takeshita, Masaru, Fukuyama, Hidehiro, Kamada, Katsuhiko, Matsumoto, Takehisa, Makino-Okamura, Chieko, Lin, Qingshun, Sakuma, Machie, Kawahara, Eiki, Yamazaki, Isato, Uchikubo-Kamo, Tomomi, Tomabechi, Yuri, Hanada, Kazuharu, Hisano, Tamao, Moriyama, Saya, Takahashi, Yoshimasa, Ito, Mutsumi, Imai, Masaki, Maemura, Tadashi, Furusawa, Yuri, Yamayoshi, Seiya, Kawaoka, Yoshihiro, Shirouzu, Mikako, Ishii, Makoto, Saya, Hideyuki, Kondo, Yasushi, Kaneko, Yuko, Suzuki, Katsuya, Fukunaga, Koichi, Takeuchi, Tsutomu

“…Several antibody therapeutics have been developed against SARS-CoV-2; however, they have attenuated neutralizing ability against variants. In this study, we…”
Get full text

Crystal structure of nanoKAZ: The mutated 19 kDa component of Oplophorus luciferase catalyzing the bioluminescent reaction with coelenterazine by Tomabechi, Yuri, Hosoya, Takamitsu, Ehara, Haruhiko, Sekine, Shun-ichi, Shirouzu, Mikako, Inouye, Satoshi

“…The 19 kDa protein (KAZ) of Oplophorus luciferase is a catalytic component, that oxidizes coelenterazine (a luciferin) with molecular oxygen to emit light. The…”
Get full text

The luciferase-based in vivo protein–protein interaction assay revealed that CHK1 promotes PP2A and PME-1 interaction by Ando, Sana, Tanaka, Keiko, Matsumoto, Maharu, Oyama, Yuki, Tomabechi, Yuri, Yamagata, Atsushi, Shirouzu, Mikako, Nakagawa, Reiko, Okimoto, Noriaki, Taiji, Makoto, Sato, Koichi, Ohama, Takashi

“…Protein phosphatase 2A (PP2A) is an essential serine/threonine protein phosphatase, and its dysfunction is involved in the onset of cancer and…”
Get full text

Chiral deaza-coelenterazine analogs for probing a substrate-binding site in the Ca.sup.2+-binding photoprotein aequorin by Inouye, Satoshi, Sumida, Yuto, Tomabechi, Yuri, Taguchi, Jumpei, Shirouzu, Mikako, Hosoya, Takamitsu

“…The Ca.sup.2+ -binding photoprotein aequorin is a complex of apoAequorin (apoprotein) and (S)-2-peroxycoelenterazine. Aequorin can be regenerated by the…”
Get full text

Reverse mutants of the catalytic 19 kDa mutant protein by Inouye, Satoshi, Sato, Jun-ichi, Sahara-Miura, Yuiko, Tomabechi, Yuri, Sumida, Yuto, Sekine, Shun-ichi, Shirouzu, Mikako, Hosoya, Takamitsu

“…Native Oplophorus luciferase (OpLase) and its catalytic 19 kDa protein (wild KAZ) show highest luminescence activity with coelenterazine (CTZ) among CTZ…”
Get full text

The NF‐κB regulator IκBβ exhibits different molecular interactivity and phosphorylation status from IκBα in an IKK2‐catalysed reaction by Shoji, Shisako, Hanada, Kazuharu, Takahashi, Masataka, Watanabe, Kazuhide, Yonemochi, Mayumi, Tomabechi, Yuri, Shirouzu, Mikako

“…Activation of the nuclear factor kappa‐light‐chain‐enhancer of activated B cells (NF‐κB) transcription factor, a central player in immune response regulation,…”
Get full text

A reproducible and scalable procedure for preparing bacterial extracts for cell-free protein synthesis by Katsura, Kazushige, Matsuda, Takayoshi, Tomabechi, Yuri, Yonemochi, Mayumi, Hanada, Kazuharu, Ohsawa, Noboru, Sakamoto, Kensaku, Takemoto, Chie, Shirouzu, Mikako

“…Cell-free protein synthesis is a useful method for preparing proteins for functional or structural analyses. However, batch-to-batch variability with regard to…”
Get full text

POLArIS, a versatile probe for molecular orientation, revealed actin filaments associated with microtubule asters in early embryos by Sugizaki, Ayana, Sato, Keisuke, Chiba, Kazuyoshi, Saito, Kenta, Kawagishi, Masahiko, Tomabechi, Yuri, Mehta, Shalin B., Ishii, Hirokazu, Sakai, Naoki, Shirouzu, Mikako, Tani, Tomomi, Terada, Sumio

“…Biomolecular assemblies govern the physiology of cells. Their function often depends on the changes in molecular arrangements of constituents, both in the…”
Get full text