Search Results - "Tolner, Berend"

Fucoidan Prolongs the Circulation Time of Dextran-Coated Iron Oxide Nanoparticles by Abdollah, Maha R. A, Carter, Thomas J, Jones, Clare, Kalber, Tammy L, Rajkumar, Vineeth, Tolner, Berend, Gruettner, Cordula, Zaw-Thin, May, Baguña Torres, Julia, Ellis, Matthew, Robson, Mathew, Pedley, R. Barbara, Mulholland, Paul, T. M. de Rosales, Rafael, Chester, Kerry Ann

“…The magnetic properties and safety of dextran-coated superparamagnetic iron oxide nanoparticles (SPIONs) have facilitated their clinical use as MRI contrast…”
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Carrier-mediated membrane transport of folates in mammalian cells by Sirotnak, F.M, Tolner, B

“…Mediated internalization of folates is required for cellular macromolecular biosynthesis. Multiple carrier-mediated mechanisms have been identified that can…”
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Production of recombinant protein in Pichia pastoris by fermentation by Tolner, Berend, Smith, Lisa, Begent, Richard H J, Chester, Kerry A

“…This protocol is applicable to recombinant protein expression by small-scale fermentation using the Pichia pastoris expression system. P. pastoris has the…”
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Generation and characterization of a diabody targeting the αvβ6 integrin by Kogelberg, Heide, Miranda, Enrique, Burnet, Jerome, Ellison, David, Tolner, Berend, Foster, Julie, Picón, Carmen, Thomas, Gareth J, Meyer, Tim, Marshall, John F, Mather, Stephen J, Chester, Kerry

“…The αvβ6 integrin is up-regulated in cancer and wound healing but it is not generally expressed in healthy adult tissue. There is increasing evidence that it…”
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A Phase I Study of Single Administration of Antibody-Directed Enzyme Prodrug Therapy with the Recombinant Anti–Carcinoembryonic Antigen Antibody-Enzyme Fusion Protein MFECP1 and a Bis-Iodo Phenol Mustard Prodrug by Mayer, Astrid, Francis, Roslyn J, Sharma, Surinder K, Tolner, Berend, Springer, Caroline J, Martin, Jan, Boxer, Geoff M, Bell, James, Green, Alan J, Hartley, John A, Cruickshank, Clare, Wren, Julie, Chester, Kerry A, Begent, Richard H J

“…Purpose: Antibody-directed enzyme prodrug therapy is a two-stage treatment whereby a tumor-targeted antibody-enzyme complex localizes in tumor for selective…”
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Clearance mechanism of a mannosylated antibody-enzyme fusion protein used in experimental cancer therapy by Kogelberg, Heide, Tolner, Berend, Sharma, Surinder K, Lowdell, Mark W, Qureshi, Uzma, Robson, Mathew, Hillyer, Tim, Pedley, R. Barbara, Vervecken, Wouter, Contreras, Roland, Begent, Richard H.J, Chester, Kerry A

“…MFECP1 is a mannosylated antibody-enzyme fusion protein used in antibody-directed enzyme prodrug therapy (ADEPT). The antibody selectively targets tumor cells…”
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Sustained Tumor Regression of Human Colorectal Cancer Xenografts Using a Multifunctional Mannosylated Fusion Protein in Antibody-Directed Enzyme Prodrug Therapy by SHARMA, Surinder K, PEDLEY, R. Barbara, BEGENT, Richard H. J, CHESTER, Kerry A, BHATIA, Jeetendra, BOXER, Geoffrey M, EL-EMIR, Ethaar, QURESHI, Uzma, TOLNER, Berend, LOWE, Helen, MICHAEL, N. Paul, MINTON, Nigel

“…Purpose: Antibody-directed enzyme prodrug therapy (ADEPT) requires highly selective antibody-mediated delivery of enzyme to tumor. MFE-CP, a multifunctional…”
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RFC-1 Gene Expression Regulates Folate Absorption in Mouse Small Intestine by Chiao, J H, Roy, K, Tolner, B, Yang, C H, Sirotnak, F M

“…Mediated folate compound transport inward in isolated luminal epithelial cells from mouse small intestine was delineated as pH-dependent and non-pH-dependent…”
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The use of a surface active agent in the protection of a fusion protein during bioprocessing by Blas, Peter, Tolner, Berend, Ward, John, Chester, Kerry, Hoare, Mike

“…The bioprocessing of a fusion protein is characterised by low yields and at a series of recovery and purification stages that leads to an overall 90% loss…”
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Development of the designed ankyrin repeat protein (DARPin) G3 for HER2 molecular imaging by Goldstein, Robert, Sosabowski, Jane, Livanos, Maria, Leyton, Julius, Vigor, Kim, Bhavsar, Gaurav, Nagy-Davidescu, Gabriela, Rashid, Mohammed, Miranda, Enrique, Yeung, Jenny, Tolner, Berend, Plückthun, Andreas, Mather, Stephen, Meyer, Tim, Chester, Kerry

“…Purpose Human epidermal growth factor receptor-2 (HER2) overexpression is a predictor of response to anti-HER2 therapy in breast and gastric cancer. Currently,…”
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Cation-selectivity of the L-glutamate transporters of Escherichia coli, Bacillus stearothermophilus and Bacillus caldotenax: dependence on the environment in which the proteins are expressed by Tolner, B, Ubbink-Kok, T, Poolman, B, Konings, W N

“…L-Glutamate transport by the H(+)-glutamate and Na(+)-glutamate symport proteins of Escherichia coli K-12 (GltPEc and GltSEc, respectively) and the…”
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IMAC capture of recombinant protein from unclarified mammalian cell feed streams by Kinna, Alexander, Tolner, Berend, Rota, Enrique Miranda, Titchener-Hooker, Nigel, Nesbeth, Darren, Chester, Kerry

“…ABSTRACT Fusion‐tag affinity chromatography is a key technique in recombinant protein purification. Current methods for protein recovery from mammalian cells…”
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Aryloxymaleimides for cysteine modification, disulfide bridging and the dual functionalization of disulfide bonds by Marculescu, Cristina, Kossen, Hanno, Morgan, Rachel E, Mayer, Patrick, Fletcher, Sally A, Tolner, Berend, Chester, Kerry A, Jones, Lyn H, Baker, James R

“…Tuning the properties of maleimide reagents holds significant promise in expanding the toolbox of available methods for bioconjugation. Herein we describe…”
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Preclinical evaluation of a novel CEA‐targeting near‐infrared fluorescent tracer delineating colorectal and pancreatic tumors by Boonstra, Martin C., Tolner, Berend, Schaafsma, Boudewijn E., Boogerd, Leonora S.F., Prevoo, Hendrica A.J.M, Bhavsar, Guarav, Kuppen, Peter J.K., Sier, Cornelis F.M., Bonsing, Bert A., Frangioni, John V., van de Velde, Cornelis J.H., Chester, Kerry A., Vahrmeijer, Alexander L.

“…Surgery is the cornerstone of oncologic therapy with curative intent. However, identification of tumor cells in the resection margins is difficult, resulting…”
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A Single Amino Acid Difference within the Folate Transporter Encoded by the Murine RFC-1 Gene Selectively Alters its Interaction with Folate Analogues by Roy, Krishnendu, Tolner, Berend, Chiao, Judy H., Sirotnak, F.M.

“…The apparent K m , but not V max , for influx of methotrexate (MTX) mediated through the plasma membrane of S180 cells by the one-carbon, reduced folate…”
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Characterization and functional expression in Escherichia coli of the sodium/proton/glutamate symport proteins of Bacillus stearothermophilus and Bacillus caldotenax by Tolner, B, Poolman, B, Konings, W N

“…The genes encoding the Na+/H+/L-glutamate symport proteins of the thermophilic organisms Bacillus stearothermophilus (gltTBs) and Bacillus caldotenax (gltTBc)…”
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Homogeneous antibody fragment conjugation by disulfide bridging introduces ‘spinostics’ by Schumacher, Felix F., Sanchania, Vishal A., Tolner, Berend, Wright, Zoë V. F., Ryan, Chris P., Smith, Mark E. B., Ward, John M., Caddick, Stephen, Kay, Christopher W. M., Aeppli, Gabriel, Chester, Kerry A., Baker, James R.

“…A major obstacle to the efficient production of antibody conjugates for therapy and diagnosis is the non-ideal performance of commonly used chemical methods…”
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Nanoparticles functionalised with recombinant single chain Fv antibody fragments (scFv) for the magnetic resonance imaging of cancer cells by Vigor, Kim L, Kyrtatos, Panagiotis G, Minogue, Shane, Al-Jamal, Khuloud T, Kogelberg, Heide, Tolner, Berend, Kostarelos, Kostas, Begent, Richard H, Pankhurst, Quentin A, Lythgoe, Mark F, Chester, Kerry A

“…Abstract Superparamagnetic iron oxide nanoparticles (SPIONs) can substantially improve the sensitivity of magnetic resonance imaging (MRI). We propose that…”
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Pre-clinical evaluation of a novel CEA-targeting near-infrared fluorescent tracer delineating colorectal and pancreatic tumors by Boonstra, Martin C., Tolner, Berend, Schaafsma, Boudewijn E., Boogerd, Leonora S.F., Prevoo, Hendrica A.J.M, Bhavsar, Guarav, Kuppen, Peter J.K., Sier, Cornelis F.M., Bonsing, Bert A., Frangioni, John V., van de Velde, Cornelis J.H., Chester, Kerry A., Vahrmeijer, Alexander L.

“…Surgery is the cornerstone of oncologic therapy with curative intent. However, identification of tumor cells in the resection margins is difficult, resulting…”
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Generation and Characterization of a Diabody Targeting the [alpha].sub.v[beta].sub.6 Integrin by Kogelberg, Heide, Miranda, Enrique, Burnet, Jerome, Ellison, David, Tolner, Berend, Foster, Julie, Picón, Carmen, Thomas, Gareth J, Meyer, Tim, Marshall, John F, Mather, Stephen J, Chester, Kerry

“…The [alpha].sub.v [beta].sub.6 integrin is up-regulated in cancer and wound healing but it is not generally expressed in healthy adult tissue. There is…”
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