Search Results - "Tolman, Joel R"

A Novel Approach to the Retrieval of Structural and Dynamic Information from Residual Dipolar Couplings Using Several Oriented Media in Biomolecular NMR Spectroscopy by Tolman, Joel R

“…The interpretation of residual dipolar couplings in terms of molecular properties of interest is complicated because of difficulties in separating structural…”
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NMR Residual Dipolar Couplings as Probes of Biomolecular Dynamics by Tolman, Joel R, Ruan, Ke

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Composite Alignment Media for the Measurement of Independent Sets of NMR Residual Dipolar Couplings by Ruan, Ke, Tolman, Joel R

“…The measurement of independent sets of NMR residual dipolar couplings (RDCs) in multiple alignment media can provide a detailed view of biomolecular structure…”
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Dipolar couplings as a probe of molecular dynamics and structure in solution by Tolman, Joel R

“…The introduction of residual dipolar coupling methodology has increased the scope of structural biological problems that can be addressed by NMR spectroscopy…”
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Structural and Dynamic Analysis of Residual Dipolar Coupling Data for Proteins by Tolman, Joel R, Al-Hashimi, Hashim M, Kay, Lewis E, Prestegard, James H

“…The measurement of residual dipolar couplings in weakly aligned proteins can potentially provide unique information on their structure and dynamics in the…”
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Conformational Dynamics Modulate Activation of the Ubiquitin Conjugating Enzyme Ube2g2 by Magala, Pearl, Bocik, William E, Majumdar, Ananya, Tolman, Joel R

“…The ubiquitin conjugating enzyme Ube2g2 together with its cognate E3 ligase gp78 catalyzes the synthesis of lysine-48 polyubiquitin chains constituting signals…”
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Selective spin inversion in solution by magic field cross polarization by Tolman, Joel R., Arbogast, Luke W.

“…[Display omitted] •A method for 2D frequency selective spin inversion is proposed.•Magic field cross polarization (CP) requires use of specific RF field…”
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Role of a non-canonical surface of Rad6 in ubiquitin conjugating activity by Kumar, Pankaj, Magala, Pearl, Geiger-Schuller, Kathryn R, Majumdar, Ananya, Tolman, Joel R, Wolberger, Cynthia

“…Rad6 is a yeast E2 ubiquitin conjugating enzyme that monoubiquitinates histone H2B in conjunction with the E3, Bre1, but can non-specifically modify histones…”
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Selective suppression of excipient signals in 2D 1H–13C methyl spectra of biopharmaceutical products by Arbogast, Luke W., Delaglio, Frank, Tolman, Joel R., Marino, John P.

“…While the use of 1 H– 13 C methyl correlated NMR spectroscopy at natural isotopic abundance has been demonstrated as feasible on protein therapeutics as large…”
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Protein dynamics from disorder by Tolman, Joel R.

“…The functions of proteins are often crucially dependent on how they move, but measuring the absolute magnitudes of protein motions hasn't been possible. A…”
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Structural biology Protein dynamics from disorder by Tolman, Joel R

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Cross-Correlated Chemical Shift Modulation:  A Signature of Slow Internal Motions in Proteins by Früh, Dominique, Tolman, Joel R, Bodenhausen, Geoffrey, Zwahlen, Catherine

“…A novel NMR experiment allows one to characterize slow motion in macromolecules. The method exploits the fact that motions, such as rotation about dihedral…”
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Unraveling long range residual dipolar coupling networks in strongly aligned proteins by Arbogast, Luke, Majumdar, Ananya, Tolman, Joel R.

“…[Display omitted] •A double resonance approach for selective inversion of individual coupled spin pairs.•Selective recoupling of complex long range residual…”
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Multinuclear NMR and Kinetic Analysis of DNA Interstrand Cross-Link Formation by Ding, Hui, Majumdar, Ananya, Tolman, Joel R, Greenberg, Marc M

“…Recently, a phenylselenyl-modified thymidine (2) was shown to produce DNA interstrand cross-links (ICLs) via two mechanisms. Photolysis of 2 generates…”
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Solution structure and dynamics of human ubiquitin conjugating enzyme Ube2g2 by Ju, Tingting, Bocik, William, Majumdar, Ananya, Tolman, Joel R.

“…Ube2g2 is an E2 enzyme which functions as part of the endoplasmic reticulum‐associated degradation (ERAD) pathway responsible for identification and…”
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NMR characterization of an engineered domain fusion between maltose binding protein and TEM1 β-lactamase provides insight into its structure and allosteric mechanism by Wright, Chapman M., Majumdar, Ananya, Tolman, Joel R., Ostermeier, Marc

“…RG13 is a 72 kDa engineered allosteric enzyme comprised of a fusion between maltose binding protein (MBP) and TEM1 β‐lactamase (BLA) for which maltose is a…”
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De novo determination of internuclear vector orientations from residual dipolar couplings measured in three independent alignment media by Ruan, Ke, Briggman, Kathryn B., Tolman, Joel R.

“…The straightforward interpretation of solution state residual dipolar couplings (RDCs) in terms of internuclear vector orientations generally requires prior…”
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Mechanism of Polyubiquitin Chain Recognition by the Human Ubiquitin Conjugating Enzyme Ube2g2 by Bocik, William E., Sircar, Aroop, Gray, Jeffrey J., Tolman, Joel R.

“…Ube2g2 is a human ubiquitin conjugating (E2) enzyme involved in the endoplasmic reticulum-associated degradation pathway, which is responsible for the…”
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De Novo Determination of Bond Orientations and Order Parameters from Residual Dipolar Couplings with High Accuracy by Briggman, Kathryn B, Tolman, Joel R

“…We report the de novo determination of 15N−H bond orientations and motional order parameters for the protein ubiquitin with high accuracy based solely on NMR…”
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HNCO-based measurement of one-bond amide ¹⁵N-¹H couplings with optimized precision by Arbogast, Luke, Majumdar, Ananya, Tolman, Joel R

“…A pair of 3D HNCO-based experiments have been developed with the aim of optimizing the precision of measurement of ¹JNH couplings. Both pulse sequences record…”
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