Humoral and cellular immune response of mice challenged with Yersinia pestis antigenic preparations

Humoral and cellular immune response of mice challenged with Yersinia pestis antigenic preparations

The plague, which is an infectious disease caused by Yersinia pestis, still threatens many populations in several countries. The worldwide increase in human plague cases and the potential use of the bacteria as a biological weapon reinforce the need to study the immunity that is induced by potential...

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Bibliographic Details
Published in:The Brazilian journal of infectious diseases Vol. 21; no. 6; pp. 620 - 626
Main Authors: Leal, Elida A., Moreira, Josimar D., Nunes, Fernanda F., Souza, Larissa R., Martins, Janaina M., Toledo, Vicente P.C., Almeida, Alzira M.P., Guimarães, Tania M.P.
Format: Journal Article
Language:English
Published: Brazil Elsevier Editora Ltda 01-11-2017
Contexto
Elsevier
Brazilian Society of Infectious Diseases
Subjects:
Analysis
Animals
Antigens
Antigens, Bacterial - immunology
CD4-CD8 Ratio
CD4-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes
Female
Flow Cytometry
Health aspects
Immune response
Immunity, Cellular
Immunogenicity, Vaccine
Immunophenotyping
INFECTIOUS DISEASES
Interferon-gamma - immunology
Interleukin-10 - immunology
Mice
Original
Plague - prevention & control
Plague Vaccine - immunology
Spleen - cytology
Spleen - immunology
Vaccines
Yersinia pestis
Yersinia pestis - immunology
Antigens
Mice
Immune response
Vaccines
Yersinia pestis
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Summary:The plague, which is an infectious disease caused by Yersinia pestis, still threatens many populations in several countries. The worldwide increase in human plague cases and the potential use of the bacteria as a biological weapon reinforce the need to study the immunity that is induced by potential vaccine candidates. To determine the immunogenicity of antigenic preparations based on the F1 protein and the total extract from Y. pestis, we assessed the role of these antigens in inducing an immune response. The immunogenicity of antigenic preparations based on the Y. pestis (YP) total extract and the Y. pestis fraction 1 capsular antigen protein (F1) was determined in Swiss-Webster mice immunized with 40μg or 20μg for each preparation. Immunophenotyping was performed by flow cytometry. Animals immunized with the YP total extract did not elicit detectable anti-F1 antibodies (Ab) in the hemaglutination/inhibition (HA/HI) test. Animals immunized with 40μg or 20μg of the F1 protein produced anti-F1 Abs, with titres ranging from 1/16 to 1/8132. The average of CD3+–CD4+ and CD3+–CD8+ T cells did not differ significantly between the groups. Neither YP total extract nor F1 protein induced a significant expression of IFN-γ and IL-10 in CD4+ T lymphocytes. In addition, F1 failed to induce IFN-γ expression in CD8+ T cells, unlike the YP total extract. The results showed that F1 protein is not an immunogenic T cell antigen, although the YP total extract (40μg dose) favoured CD8+ T cell-mediated cellular immunity.
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ISSN:1413-8670
1678-4391
1678-4391
DOI:10.1016/j.bjid.2017.09.001