Mitochondrial metabolism of guanine nucleotides possible role of guanosine

The catabolism of intramitochondrial guanine nucleotides was examined. During 30 min incubation of rat liver mitochondria at 37°C in the presence of oligomycin and carboxyatractyloside, guanine and xanthine were formed and appeared in the medium. Under these conditions, the direct conversion of GMP...

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Bibliographic Details
Published in:	FEBS letters Vol. 248; no. 1-2; pp. 182 - 184
Main Authors:	Ziegler, Mathias, Dubiel, Wolfgang, Pimenov, Alexander M., Tikhonov, Yuri V., Toguzov, Rusian T., Henke, Wolfgang, Gerber, Gerhard
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 08-05-1989 Elsevier
Subjects:	Analytical, structural and metabolic biochemistry Animals Atractyloside - analogs & derivatives Atractyloside - pharmacology Biological and medical sciences carboxyatractyloside CAT Chromatography, High Pressure Liquid Fundamental and applied biological sciences. Psychology Guanine - biosynthesis Guanine Nucleotides - metabolism Guanosine Guanosine - metabolism HPLC Inosine - metabolism Male Mitochondria Mitochondria, Liver - metabolism Nucleic acids Nucleic bases, nucleotides Oligomycins - pharmacology Purine nucleotide metabolism Rat liver Rats Rats, Inbred Strains Subcellular Fractions - metabolism Temperature Xanthine Xanthines - biosynthesis carboxyatractyloside Mitochondria Purine nucleotide metabolism Rat liver Guanosine CAT HPLC Radiolabelling Rat Enzyme Liver Rodentia Guanine nucleotide HPLC chromatography Biosynthesis Hypoxanthine phosphoribosyltransferase Xanthine Metabolism Vertebrata Mammalia Enzymatic digestion Nucleotide
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Summary:	The catabolism of intramitochondrial guanine nucleotides was examined. During 30 min incubation of rat liver mitochondria at 37°C in the presence of oligomycin and carboxyatractyloside, guanine and xanthine were formed and appeared in the medium. Under these conditions, the direct conversion of GMP to guanine by hypoxanthine—guanine phosphoribosyltransferase is suggested to be the main catabolic route within the organelles. Only very small amounts of guanosine were produced and detected both inside and outside the organelles. [14C]Guanosine and [14C]inosine were taken up by the mitochondria. Therefore, guanosine is suggested to be a precursor of intramitochondrial guanine nucleotides.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(89)80457-0