Search Results - "Toftgaard Nielsen, Alex"

Comparative study on aptamers as recognition elements for antibiotics in a label-free all-polymer biosensor by Daprà, Johannes, Lauridsen, Lasse Holm, Nielsen, Alex Toftgaard, Rozlosnik, Noemi

“…We present an all-polymer electrochemical microfluidic biosensor using Topas® as substrate and a conductive polymer bilayer as electrode material. The…”
Get full text

Engineering of high yield production of L-serine in Escherichia coli by Mundhada, Hemanshu, Schneider, Konstantin, Christensen, Hanne Bjerre, Nielsen, Alex Toftgaard

“…ABSTRACT L‐serine is a widely used amino acid that has been proposed as a potential building block biochemical. The high theoretical yield from glucose makes a…”
Get full text

CRMAGE: CRISPR Optimized MAGE Recombineering by Ronda, Carlotta, Pedersen, Lasse Ebdrup, Sommer, Morten O. A., Nielsen, Alex Toftgaard

“…A bottleneck in metabolic engineering and systems biology approaches is the lack of efficient genome engineering technologies. Here, we combine CRISPR/Cas9 and…”
Get full text

Production of zosteric acid and other sulfated phenolic biochemicals in microbial cell factories by Jendresen, Christian Bille, Nielsen, Alex Toftgaard

“…Biological production and application of a range of organic compounds is hindered by their limited solubility and toxicity. This work describes a process for…”
Get full text

Distribution of Bacterial Growth Activity in Flow-Chamber Biofilms by STERNBERG, C, CHRISTENSEN, B. B, JOHANSEN, T, NIELSEN, A. T, ANDERSEN, J. B, GIVSKOV, M, MOLIN, S

“…Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit…”
Get full text

Highly Active and Specific Tyrosine Ammonia-Lyases from Diverse Origins Enable Enhanced Production of Aromatic Compounds in Bacteria and Saccharomyces cerevisiae by Jendresen, Christian Bille, Stahlhut, Steen Gustav, Li, Mingji, Gaspar, Paula, Siedler, Solvej, Förster, Jochen, Maury, Jérôme, Borodina, Irina, Nielsen, Alex Toftgaard

“…Phenylalanine and tyrosine ammonia-lyases form cinnamic acid and p-coumaric acid, which are precursors of a wide range of aromatic compounds of…”
Get full text

Molecular tools for study of biofilm physiology by Christensen, B B, Sternberg, C, Andersen, J B, Palmer, Jr, R J, Nielsen, A T, Givskov, M, Molin, S

Get more information

CRISPRi-mediated metabolic switch enables concurrent aerobic and synthetic anaerobic fermentations in engineered consortium by Rong, Yixin, Frey, Adrian, Özdemir, Emre, Sainz de la Maza Larrea, Arrate, Li, Songyuan, Nielsen, Alex Toftgaard, Jensen, Sheila Ingemann

“…Replacing petrochemicals with compounds from bio-based manufacturing processes remains an important part of the global effort to move towards a sustainable…”
Get full text

Accelerating genome editing in CHO cells using CRISPR Cas9 and CRISPy, a web-based target finding tool by Ronda, Carlotta, Pedersen, Lasse Ebdrup, Hansen, Henning Gram, Kallehauge, Thomas Beuchert, Betenbaugh, Michael J., Nielsen, Alex Toftgaard, Kildegaard, Helene Faustrup

“…ABSTRACT Chinese hamster ovary (CHO) cells are widely used in the biopharmaceutical industry as a host for the production of complex pharmaceutical proteins…”
Get full text

Enhanced protein and biochemical production using CRISPRi-based growth switches by Li, Songyuan, Jendresen, Christian Bille, Grünberger, Alexander, Ronda, Carlotta, Jensen, Sheila Ingemann, Noack, Stephan, Nielsen, Alex Toftgaard

“…Production of proteins and biochemicals in microbial cell factories is often limited by carbon and energy spent on excess biomass formation. To address this…”
Get full text

Lab-on-a-disc platform for screening of genetically modified E. coli cells via cell-free electrochemical detection of p-Coumaric acid by Sanger, Kuldeep, Zór, Kinga, Bille Jendresen, Christian, Heiskanen, Arto, Amato, Letizia, Toftgaard Nielsen, Alex, Boisen, Anja

“…•Lab-on-a-disc device was developed for screening of genetically modified strains.•p-Coumaric acid was measured using electrodes fabricated on plastic…”
Get full text

Increasing production yield of tyrosine and mevalonate through inhibition of biomass formation by Li, Songyuan, Jendresen, Christian Bille, Nielsen, Alex Toftgaard

“…[Display omitted] •Reduction of E. coli biomass formation results in higher product yield and titer.•Effect of nutrient limitation and growth inhibitors were…”
Get full text

Genetic toolbox for controlled expression of functional proteins in Geobacillus spp by Pogrebnyakov, Ivan, Jendresen, Christian Bille, Nielsen, Alex Toftgaard

“…Species of genus Geobacillus are thermophilic bacteria and play an ever increasing role as hosts for biotechnological applications both in academia and…”
Get full text

A dual-reporter system for investigating and optimizing protein translation and folding in E. coli by Zutz, Ariane, Hamborg, Louise, Pedersen, Lasse Ebdrup, Kassem, Maher M., Papaleo, Elena, Koza, Anna, Herrgård, Markus J., Jensen, Sheila Ingemann, Teilum, Kaare, Lindorff-Larsen, Kresten, Nielsen, Alex Toftgaard

“…Strategies for investigating and optimizing the expression and folding of proteins for biotechnological and pharmaceutical purposes are in high demand. Here,…”
Get full text

Genome-wide systematic identification of methyltransferase recognition and modification patterns by Jensen, Torbjørn Ølshøj, Tellgren-Roth, Christian, Redl, Stephanie, Maury, Jérôme, Jacobsen, Simo Abdessamad Baallal, Pedersen, Lasse Ebdrup, Nielsen, Alex Toftgaard

“…Genome-wide analysis of DNA methylation patterns using single molecule real-time DNA sequencing has boosted the number of publicly available methylomes…”
Get full text

Exploiting the potential of gas fermentation by Redl, Stephanie, Diender, Martijn, Jensen, Torbjørn Ølshøj, Sousa, Diana Z., Nielsen, Alex Toftgaard

“…[Display omitted] •Overview of conversion of gasified biomass and industrial gases into chemicals.•Review of industrial waste gases and gasification of biomass…”
Get full text

Surface Enhanced Raman Scattering for Quantification of p‑Coumaric Acid Produced by Escherichia coli by Morelli, Lidia, Zór, Kinga, Jendresen, Christian Bille, Rindzevicius, Tomas, Schmidt, Michael Stenbæk, Nielsen, Alex Toftgaard, Boisen, Anja

“…The number of newly developed genetic variants of microbial cell factories for production of biochemicals has been rapidly growing in recent years, leading to…”
Get full text

An improved integrative GFP-based vector for genetic engineering of Parageobacillus thermoglucosidasius facilitates the identification of a key sporulation regulator by Millgaard, Marie, Bidart, Gonzalo Nahuel, Pogrebnyakov, Ivan, Nielsen, Alex Toftgaard, Welner, Ditte Hededam

“…Parageobacillus thermoglucosidasius is a thermophilic Gram-positive bacterium, which is a promising host organism for sustainable bio-based production…”
Get full text

Simultaneous quantification of multiple bacterial metabolites using surface-enhanced Raman scattering by Morelli, Lidia, Centorbi, Francesca Alessandra, Ilchenko, Oleksii, Jendresen, Christian Bille, Demarchi, Danilo, Nielsen, Alex Toftgaard, Zór, Kinga, Boisen, Anja

“…Given the commercial importance of the compounds produced by genetically modified organisms, there is a need for screening methods which facilitate the…”
Get more information

Generation of an E. coli platform strain for improved sucrose utilization using adaptive laboratory evolution by Mohamed, Elsayed T, Mundhada, Hemanshu, Landberg, Jenny, Cann, Isaac, Mackie, Roderick I, Nielsen, Alex Toftgaard, Herrgård, Markus J, Feist, Adam M

“…Sucrose is an attractive industrial carbon source due to its abundance and the fact that it can be cheaply generated from sources such as sugarcane. However,…”
Get full text