Search Results - "Toettcher, Jared E"

Using Optogenetics to Interrogate the Dynamic Control of Signal Transmission by the Ras/Erk Module by Toettcher, Jared E., Weiner, Orion D., Lim, Wendell A.

“…The complex, interconnected architecture of cell-signaling networks makes it challenging to disentangle how cells process extracellular information to make…”
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Spatiotemporal Control of Intracellular Phase Transitions Using Light-Activated optoDroplets by Shin, Yongdae, Berry, Joel, Pannucci, Nicole, Haataja, Mikko P., Toettcher, Jared E., Brangwynne, Clifford P.

“…Phase transitions driven by intrinsically disordered protein regions (IDRs) have emerged as a ubiquitous mechanism for assembling liquid-like RNA/protein (RNP)…”
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A bright future: optogenetics to dissect the spatiotemporal control of cell behavior by Goglia, Alexander G, Toettcher, Jared E

“…Cells sense, process, and respond to extracellular information using signaling networks: collections of proteins that act as precise biochemical sensors. These…”
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Mapping Local and Global Liquid Phase Behavior in Living Cells Using Photo-Oligomerizable Seeds by Bracha, Dan, Walls, Mackenzie T., Wei, Ming-Tzo, Zhu, Lian, Kurian, Martin, Avalos, José L., Toettcher, Jared E., Brangwynne, Clifford P.

“…Liquid-liquid phase separation plays a key role in the assembly of diverse intracellular structures. However, the biophysical principles by which phase…”
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Optogenetic regulation of engineered cellular metabolism for microbial chemical production by Zhao, Evan M., Zhang, Yanfei, Mehl, Justin, Park, Helen, Lalwani, Makoto A., Toettcher, Jared E., Avalos, José L.

“…Finely tuned optogenetic control of engineered Saccharomyces cerevisiae enhances the biosynthesis of valuable products such as isobutanol in laboratory-scale…”
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Tracing Information Flow from Erk to Target Gene Induction Reveals Mechanisms of Dynamic and Combinatorial Control by Wilson, Maxwell Z., Ravindran, Pavithran T., Lim, Wendell A., Toettcher, Jared E.

“…Cell signaling networks coordinate specific patterns of protein expression in response to external cues, yet the logic by which signaling pathway activity…”
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Light-based control of metabolic flux through assembly of synthetic organelles by Zhao, Evan M., Suek, Nathan, Wilson, Maxwell Z., Dine, Elliot, Pannucci, Nicole L., Gitai, Zemer, Avalos, José L., Toettcher, Jared E.

“…To maximize a desired product, metabolic engineers typically express enzymes to high, constant levels. Yet, permanent pathway activation can have undesirable…”
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The Spatiotemporal Limits of Developmental Erk Signaling by Johnson, Heath E., Goyal, Yogesh, Pannucci, Nicole L., Schüpbach, Trudi, Shvartsman, Stanislav Y., Toettcher, Jared E.

“…Animal development is characterized by signaling events that occur at precise locations and times within the embryo, but determining when and where such…”
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Light-switchable transcription factors obtained by direct screening in mammalian cells by Zhu, Liyuan, McNamara, Harold M., Toettcher, Jared E.

“…Optogenetic tools can provide fine spatial and temporal control over many biological processes. Yet the development of new light-switchable protein variants…”
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Light-based feedback for controlling intracellular signaling dynamics by Toettcher, Jared E, Gong, Delquin, Lim, Wendell A, Weiner, Orion D

“…Feedback control of a light-gated protein-protein interaction is used to deliver precisely tuned intracellular signals to cultured cells. The ability to apply…”
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Optogenetic control of protein binding using light-switchable nanobodies by Gil, Agnieszka A., Carrasco-López, César, Zhu, Liyuan, Zhao, Evan M., Ravindran, Pavithran T., Wilson, Maxwell Z., Goglia, Alexander G., Avalos, José L., Toettcher, Jared E.

“…A growing number of optogenetic tools have been developed to reversibly control binding between two engineered protein domains. In contrast, relatively few…”
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Viral DNA Sensors IFI16 and Cyclic GMP-AMP Synthase Possess Distinct Functions in Regulating Viral Gene Expression, Immune Defenses, and Apoptotic Responses during Herpesvirus Infection by Diner, Benjamin A, Lum, Krystal K, Toettcher, Jared E, Cristea, Ileana M

“…The human interferon-inducible protein IFI16 is an important antiviral factor that binds nuclear viral DNA and promotes antiviral responses. Here, we define…”
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Four Key Steps Control Glycolytic Flux in Mammalian Cells by Tanner, Lukas Bahati, Goglia, Alexander G, Wei, Monica H, Sehgal, Talen, Parsons, Lance R, Park, Junyoung O, White, Eileen, Toettcher, Jared E, Rabinowitz, Joshua D

“…Altered glycolysis is a hallmark of diseases including diabetes and cancer. Despite intensive study of the contributions of individual glycolytic enzymes,…”
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Protein Phase Separation Provides Long-Term Memory of Transient Spatial Stimuli by Dine, Elliot, Gil, Agnieszka A, Uribe, Giselle, Brangwynne, Clifford P, Toettcher, Jared E

“…Protein/RNA clusters arise frequently in spatially regulated biological processes, from the asymmetric distribution of P granules and PAR proteins in…”
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Rapid and reversible dissolution of biomolecular condensates using light-controlled recruitment of a solubility tag by Brumbaugh-Reed, Ellen H., Gao, Yang, Aoki, Kazuhiro, Toettcher, Jared E.

“…Biomolecular condensates are broadly implicated in both normal cellular regulation and disease. Consequently, several chemical biology and optogenetic…”
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The promise of optogenetics in cell biology: interrogating molecular circuits in space and time by Lim, Wendell A, Toettcher, Jared E, Voigt, Christopher A, Weiner, Orion D

“…Optogenetic modules offer cell biologists unprecedented new ways to poke and prod cells. The combination of these precision perturbative tools with…”
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Development of light-responsive protein binding in the monobody non-immunoglobulin scaffold by Carrasco-López, César, Zhao, Evan M., Gil, Agnieszka A., Alam, Nathan, Toettcher, Jared E., Avalos, José L.

“…Monobodies are synthetic non-immunoglobulin customizable protein binders invaluable to basic and applied research, and of considerable potential as future…”
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Optimizing photoswitchable MEK by Patel, Aleena L., Yeung, Eyan, McGuire, Sarah E., Wu, Andrew Y., Toettcher, Jared E., Burdine, Rebecca D., Shvartsman, Stanislav Y.

“…Optogenetic approaches are transforming quantitative studies of cell-signaling systems. A recently developed photoswitchable mitogen-activated protein kinase…”
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Optogenetic control of the Bicoid morphogen reveals fast and slow modes of gap gene regulation by Singh, Anand P., Wu, Ping, Ryabichko, Sergey, Raimundo, João, Swan, Michael, Wieschaus, Eric, Gregor, Thomas, Toettcher, Jared E.

“…Developmental patterning networks are regulated by multiple inputs and feedback connections that rapidly reshape gene expression, limiting the information that…”
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A self-exciting point process to study multicellular spatial signaling patterns by Verma, Archit, Jena, Siddhartha G., Isakov, Danielle R., Aoki, Kazuhiro, Toettcher, Jared E., Engelhardt, Barbara E.

“…Multicellular organisms rely on spatial signaling among cells to drive their organization, development, and response to stimuli. Several models have been…”
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