Genetic variability in the ITS and IGS regions of the ribosomal DNA of Acremonium cavaraeanum exhibiting antimicrobial activity

Endophytic microorganisms represent promising alternatives for obtaining new drugs of biotechnological importance. In this study, the endophytic species Acremonium cavaraeanum (A1a) isolated from Cocos nucifera was cultivated for the production of secondary metabolites, and its extracts and fraction...

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Published in:	Genetics and molecular research Vol. 12; no. 4; pp. 6983 - 6995
Main Authors:	Todaro, A R, Nascimento, V X, Souza, N C C, Silva, P P, Santos, J M, Ramalho, E A V F, Melo, T V C, Silva, I S M, Lins Júnior, G S, Bastos, M L A, Farias, D F, Souza, P C V, Carvalho, A F U, Silva, M C S, Almeida, R M R G, Ramalho Neto, E
Format:	Journal Article
Language:	English
Published:	Brazil 20-12-2013
Subjects:	Acremonium Acremonium - genetics Acremonium - isolation & purification Anti-Bacterial Agents - isolation & purification Chromosome Mapping Cocos - microbiology Cocos nucifera DNA, Ribosomal Spacer - genetics Fungal Proteins - pharmacology Gram-Negative Bacteria - drug effects Gram-Positive Bacteria - drug effects Liquid-Liquid Extraction Microbial Sensitivity Tests Polymorphism, Restriction Fragment Length Staphylococcus aureus
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Summary:	Endophytic microorganisms represent promising alternatives for obtaining new drugs of biotechnological importance. In this study, the endophytic species Acremonium cavaraeanum (A1a) isolated from Cocos nucifera was cultivated for the production of secondary metabolites, and its extracts and fractions were evaluated by the dilution method (MIC). The EtOAc extracts and MeOH fractions were tested against Gram-positive and -negative bacteria, and had an MIC of 125 µg/mL when evaluated in the EtOAc extract (EBI). The EtOAc extract (EBII) had an MIC of 62.25 µg/mL for Staphylococcus aureus and an MIC between 125 and 250 µg/mL for Gram-negative bacteria. The methanolic fractions showed activity with MIC between 125 and 250 µg/mL for all bacteria tested. The IGS region of the rDNA repeat unit of genomic DNA was analyzed by PCR/RFLPs, including endonucleases PstI, BamHII, HinfI, and EcoRI. The physical maps showed different restriction sites for the 6 Acremonium sp isolates, and revealed 5 RFLP patterns. The results showed that isolates of the same Acremonium species exhibited variation in this specific region. The sequences of ITS1-5.8S-ITS2 regions were aligned by Clustal W using the neighbor joining method, which grouped the isolates into 5 distinct clusters. This study aimed to evaluate the genetic diversity of A. cavaraeanum crops exhibiting antibacterial activity. The results of this study indicate that different fungal genetic isolates have biotechnological potential for the production of active bio-compounds against several human pathogens.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1676-5680 1676-5680
DOI:	10.4238/2013.December.20.1