Investigations on the possible impact of a glyphosate‐containing herbicide on ruminal metabolism and bacteria in vitro by means of the ‘Rumen Simulation Technique’

Aims This study was performed in a well‐established in vitro model to investigate whether the application of a glyphosate‐containing herbicide might affect the bacterial communities and some biochemical parameters in a cow's rumen. Methods and Results The test item was applied in two concentrat...

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Bibliographic Details
Published in:	Journal of applied microbiology Vol. 121; no. 3; pp. 644 - 656
Main Authors:	Riede, S., Toboldt, A., Breves, G., Metzner, M., Köhler, B., Bräunig, J., Schafft, H., Lahrssen‐Wiederholt, M., Niemann, L.
Format:	Journal Article
Language:	English
Published:	England Oxford University Press 01-09-2016
Subjects:	Acid production Ammonia Animals Antibiotics Bacteria Bacteria - classification Bacteria - metabolism Biochemistry Cattle - metabolism Cattle - microbiology Clostridia Clostridium Clostridium - classification Clostridium - drug effects Clostridium - growth & development Clostridium sporogenes Communities Composition Computer simulation Diet Fatty acids Fatty Acids - analysis Female Fermentation Gastric Juice - microbiology Gastrointestinal Microbiome - drug effects Genetic analysis Glycine - analogs & derivatives Glycine - toxicity Glyphosate Herbicides Herbicides - toxicity In Vitro Techniques Metabolism microbial communities Microbiology Microorganisms Rumen Rumen - metabolism Rumen - microbiology Rumen Simulation Technique ruminal metabolism microbial communities Rumen Simulation Technique Clostridia glyphosate ruminal metabolism
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Summary:	Aims This study was performed in a well‐established in vitro model to investigate whether the application of a glyphosate‐containing herbicide might affect the bacterial communities and some biochemical parameters in a cow's rumen. Methods and Results The test item was applied in two concentrations (high and low) for 5 days. In a second trial, fermentation vessels were inoculated with Clostridium sporogenes before the high dose was applied. Effluents were analysed by biochemical, microbiological and genetic methods. A marginal increase in short‐chain fatty acid production and a reduction in NH3‐N were observed. There were minor and rather equivocal changes in the composition of ruminal bacteria but no indications of a shift towards a more frequent abundance of pathogenic Clostridia species. Clostridium sporogenes counts declined consistently. Conclusions No adverse effects of the herbicide on ruminal metabolism or composition of the bacterial communities could be detected. In particular, there was no evidence of a suspected stimulation of Clostridia growth. Significance and Impact of the Study Antibiotic activity of glyphosate resulting in microbial imbalances has been postulated. In this exploratory study, however, intraruminal application of concentrations reflecting potential exposure of dairy cows or beef cattle did not exhibit significant effects on bacterial communities in a complex in vitro system. The low number of replicates (n = 3/dose) may leave some uncertainty.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1364-5072 1365-2672
DOI:	10.1111/jam.13190