Effect of 2,4-dinitrophenol on the rat liver respiratory activity and ATP content after hypothermic storage and following reperfusion

The influence of oxidative phosphorylation uncoupler 2,4-dinitrophenol (DNP) presence in preserving solution on the rat liver respiratory activity and ATP content after 18 h of hypothermic storage (HS) and following normothermic reperfusion (NR) was investigated. DNP presence on the HS stage led to...

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Bibliographic Details
Published in:	Ukrains'kyi biokhimichnyi zhurnal (1999 ) Vol. 80; no. 2; p. 101
Main Authors:	Cherkashyna, D V, Tkachova, O M, Somov, O Iu, Semenchenko, O A, Lebedyns'kyĭ, O S, Petrenko, O Iu
Format:	Journal Article
Language:	Ukrainian
Published:	Ukraine 01-03-2008
Subjects:	2,4-Dinitrophenol - pharmacology Adenosine Triphosphate - metabolism Alanine Transaminase - metabolism Animals Aspartate Aminotransferases - metabolism Female Hypothermia, Induced In Vitro Techniques Lipid Peroxidation Liver - blood supply Liver - drug effects Liver - metabolism Oxidative Phosphorylation Oxygen Consumption - drug effects Rats Reperfusion
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Summary:	The influence of oxidative phosphorylation uncoupler 2,4-dinitrophenol (DNP) presence in preserving solution on the rat liver respiratory activity and ATP content after 18 h of hypothermic storage (HS) and following normothermic reperfusion (NR) was investigated. DNP presence on the HS stage led to decrease of ATP level as compared with the control. After DNP removal during NR the gradual recovery of oxidative phosphorylation coupling occurred. This fact resulted in improvement of mitochondrial functional state (V4 respiration rate decrease, respiratory control and ATP level increase).