Search Results - "Tiwari, Sapana"

Development and Comparative Evaluation of a Plate Enzyme-Linked Immunosorbent Assay Based on Recombinant Outer Membrane Antigens Omp28 and Omp31 for Diagnosis of Human Brucellosis by Tiwari, Sapana, Kumar, Ashu, Thavaselvam, Duraipandian, Mangalgi, Smita, Rathod, Vedika, Prakash, Archana, Barua, Anita, Arora, Sonia, Sathyaseelan, Kannusamy

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Isolation & characterization of Brucella melitensis isolated from patients suspected for human brucellosis in India by Barua, Anita, Kumar, Ashu, Thavaselvam, Duraipandian, Mangalgi, Smita, Prakash, Archana, Tiwari, Sapana, Arora, Sonia, Sathyaseelan, Kannusamy

“…Background & objectives: Brucellosis is endemic in the southern part of India. A combination of biochemical, serological and molecular methods is required for…”
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Cloning and expression of the immunoreactive Brucella melitensis 28 kDa outer-membrane protein (Omp28) encoding gene and evaluation of the potential of Omp28 for clinical diagnosis of brucellosis by THAVASELVAM, Duraipandian, KUMAR, Ashu, TIWARI, Sapana, MISHRA, Manvi, PRAKASH, Archana

“…Brucellosis is a disease caused by Gram-negative, facultative, intracellular bacteria belonging to the genus Brucella. It is an emerging zoonosis, and an…”
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Isolation, identification and characterization of Burkholderia pseudomallei from soil of coastal region of India by Prakash, Archana, Thavaselvam, Duraipandian, Kumar, Ashu, Kumar, Ajith, Arora, Sonia, Tiwari, Sapana, Barua, Anita, Sathyaseelan, Kannusamy

“…Melioidosis is an emerging infectious disease caused by a free living soil dwelling Gram-negative bacterium Burkholderia pseudomallei . The disease is endemic…”
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Cloning and expression of the immunoreactive Brucella melitensis 28 kDa outer-membrane protein (Omp28) encoding geneand evaluation of the potential of Omp28 for clinical diagnosis of brucellosis by Thavaselvam, Duraipandian, Kumar, Ashu, Tiwari, Sapana, Mishra, Manvi, Prakash, Archana

“…Brucellosis is a disease caused by Gram-negative, facultative, intracellularbacteria belonging to the genus Brucella . It is an emerging zoonosis,and an…”
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Optimization and efficient purification of recombinant Omp28 protein of Brucella melitensis using Triton X-100 and β-mercaptoethanol by Kumar, Ashu, Tiwari, Sapana, Thavaselvam, Duraipandian, Sathyaseelan, Kannusamy, Prakash, Archana, Barua, Anita, Arora, Sonia, Kameswara Rao, M.

“…► Expression of recombinant Omp28 protein of Brucella melitensis in pET28a and pQE30UA vectors. ► Use of 1% Triton X-100 and 20mM β-mercaptoethanol in buffers…”
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Optimization and efficient purification of recombinant Omp28 protein of Brucella melitensis using Triton X-100 and I2-mercaptoethanol by Kumar, Ashu, Tiwari, Sapana, Thavaselvam, Duraipandian, Sathyaseelan, Kannusamy, Prakash, Archana, Barua, Anita, Arora, Sonia, Kameswara Rao, M

“…The high level expression of recombinant proteins in Escherichia coli often leads to the formation of inclusion bodies that contain most of the expressed…”
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