Genetic Identification and Antimicrobial Activity of Streptomyces sp. Strain Je 1–6 Isolated from Rhizosphere Soil of Juniperus excelsa Bieb

Genetic Identification and Antimicrobial Activity of Streptomyces sp. Strain Je 1–6 Isolated from Rhizosphere Soil of Juniperus excelsa Bieb

Actinobacteria isolated from poorly explored biotopes are of interest as producers of biologically active compounds. A report is given about the producer of lydicamycin Je 1–6 isolated from the rhizosphere soil of J. excelsa Bieb. collected from the Crimean Peninsula, Ukraine, that demonstrated its...

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Bibliographic Details
Published in:Cytology and genetics Vol. 55; no. 1; pp. 28 - 35
Main Authors: Tistechok, S. I., Tymchuk, I. V., Korniychuk, O. P., Fedorenko, V. O., Luzhetskyy, A. M., Gromyko, O. M.
Format: Journal Article
Language:English
Published: Moscow Pleiades Publishing 2021
Springer Nature B.V
Subjects:
Antimicrobial activity
Bioactive compounds
Biological activity
Biomedical and Life Sciences
Biomedicine
Clinical isolates
Congeners
Gram-positive bacteria
Homology
Human Genetics
RecA protein
Rhizosphere
RpoB protein
rRNA 16S
Sequence analysis
Streptomyces
MLSA
phylogenetic analysis
lydicamycin
housekeeping genes
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Summary:Actinobacteria isolated from poorly explored biotopes are of interest as producers of biologically active compounds. A report is given about the producer of lydicamycin Je 1–6 isolated from the rhizosphere soil of J. excelsa Bieb. collected from the Crimean Peninsula, Ukraine, that demonstrated its antagonistic activity against gram-positive bacteria. Based on the 16S rRNA gene sequence analysis, the strain Je 1–6 was affiliated to the Streptomyces genus. The crude extract from the biomass of this strain showed its activity against gram-positive bacteria, including polyresistant clinical isolates of Staphylococcus sp. The dereplication analysis of the extract of the strain Je 1–6 revealed lydicamycin and its congeners: 30-demethyllydicamycin, 30-demethyl-8-deoxylydicamycin, and 8-deoxylydicamycin. MLSA analysis based on the 16S rRNA gene and five housekeeping genes atpD, gyrB, rpoB, recA, and trpB of the strain Je 1–6 showed a high level of homology with Streptomyces sp. ID38640.
ISSN:0095-4527
1934-9440
DOI:10.3103/S0095452721010138