Search Results - "Tippmann, Eric M."

A Critical Examination of Escherichia coli Esterase Activity by Antonczak, Alicja K., Simova, Zuzana, Tippmann, Eric M.

“…The ability of Escherichia coli to grow on a series of acetylated and glycosylated compounds has been investigated. It is surmised that E. coli maintains low…”
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In-frame amber stop codon replacement mutagenesis for the directed evolution of proteins containing non-canonical amino acids: identification of residues open to bio-orthogonal modification by Arpino, James A J, Baldwin, Amy J, McGarrity, Adam R, Tippmann, Eric M, Jones, D Dafydd

“…Expanded genetic code approaches are a powerful means to add new and useful chemistry to proteins at defined residues positions. One such use is the…”
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Genetically Encoded Diazirine Photocrosslinker in Escherichia coli by Tippmann, Eric M, Liu, Wenshe, Summerer, Daniel, Mack, Antha V, Schultz, Peter G

“…Directed evolution. An orthogonal amber suppressor tRNA/aminoacyl–tRNA synthetase pair was evolved that allows the site‐specific incorporation of…”
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Advances in the mechanism and understanding of site-selective noncanonical amino acid incorporation by Antonczak, Alicja K, Morris, Josephine, Tippmann, Eric M

“…► Important topics in NAA biological chemistry are: chemical functionality, fidelity, and efficiency. ► Tyrosyl and pyrrolysyl enzymes are amendable to an…”
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Importance of single molecular determinants in the fidelity of expanded genetic codes by Antonczak, Alicja K., Simova, Zuzana, Yonemoto, Isaac T., Bochtler, Matthias, Piasecka, Anna, Czapińska, Honorata, Brancale, Andrea, Tippmann, Eric M., Huber, Robert, Planck, Max

“…The site-selective encoding of noncanonical amino acids (NAAs) is a powerful technique for the installation of novel chemical functional groups in proteins…”
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Residue choice defines efficiency and influence of bioorthogonal protein modification via genetically encoded strain promoted Click chemistry by Reddington, Samuel C, Tippmann, Eric M, Jones, D Dafydd

“…GFP and a FRET compatible dye were used to assess the influence of genetically encoded aryl azide positioning on Click chemistry-based protein conjugation…”
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Correction to: Genetically encoded photochemical covalent crosslinking within the Hcp-1 self-assembling bacterial secretion machinery by Antonczak, Alicja K., Milholland, Kedric, Tippmann, Eric M.

“…The authors declare that the contributing author, Antonczak A.K., had no active academic address at or after the time of publication…”
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Genetically encoded photochemical covalent crosslinking within the Hcp-1 self-assembling bacterial secretion machinery by Antonczak, Alicja K., Milholland, Kedric, Tippmann, Eric M.

“…The target protein, Hcp1, was first described as part of the bacterial Type VI secretion system from Pseudomonas aeruginosa. The protein first self-assembles…”
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Probing Eudesmane Cation−π Interactions in Catalysis by Aristolochene Synthase with Non-canonical Amino Acids by Faraldos, Juan A, Antonczak, Alicja K, González, Verónica, Fullerton, Rebecca, Tippmann, Eric M, Allemann, Rudolf K

“…Stabilization of the reaction intermediate eudesmane cation (3) through interaction with Trp 334 during catalysis by aristolochene synthase from Penicillium…”
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Different Photochemical Events of a Genetically Encoded Phenyl Azide Define and Modulate GFP Fluorescence by Reddington, Samuel C., Rizkallah, Pierre J., Watson, Peter D., Pearson, Rachel, Tippmann, Eric M., Jones, D. Dafydd

“…Expanding the genetic code opens new avenues to modulate protein function in real time. By genetically incorporating photoreactive phenyl azide, the…”
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Directed evolution of GFP with non-natural amino acids identifies residues for augmenting and photoswitching fluorescence by Reddington, Samuel C, Baldwin, Amy J, Thompson, Rebecca, Brancale, Andrea, Tippmann, Eric M, Jones, D Dafydd

“…Genetic code reprogramming allows proteins to sample new chemistry through the defined and targeted introduction of non-natural amino acids (nAAs). Many useful…”
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Aryl Azide Photochemistry in Defined Protein Environments by Morris, Josephine L, Reddington, Samuel C, Murphy, Damien M, Jones, D. Dafydd, Platts, James A, Tippmann, Eric M

“…A genetically encoded precursor to an aryl nitrene, para-azidophenylalanine, was introduced site specifically into proteins to deduce if distinct environments…”
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A genetically encoded metallocene containing amino acid by Tippmann, Eric M., Schultz, Peter G.

“…Redox active amino acids, cofactors, and metal ions are involved in a large number of catalytic, electron transfer, and regulatory processes in biology…”
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juggernauts of biology by Yonemoto, Isaac T, Tippmann, Eric M

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Expanded chemical diversity sampling through whole protein evolution by Baldwin, Amy J, Arpino, James A J, Edwards, Wayne R, Tippmann, Eric M, Jones, D Dafydd

“…A directed evolution method has been developed that allows random substitution of a contiguous trinucleotide sequence for TAG throughout a target gene for use…”
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Weinreb Amides in Carbene Chemistry: A Time-Resolved IR Investigation into a Potential Intramolecular Stabilization Mechanism by Evans, Anthony S, Sundaram, G. S. M, Saβmannshausen, Jörg, Toscano, John P, Tippmann, Eric M

“…A chloromethylhydroxamiccarbene was generated photochemically in an attempt to form an intramolecularly stabilized carbene. A rapidly formed intermediate at…”
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Synthesis of bioorthogonal and crosslinking amino acids for use in peptide synthesis by Sundaram, G. S. M, Morgan, Ian R, Tippmann, Eric M

“…The ability to incorporate non-canonical amino acids into proteins by genetic or chemical methods allows one to introduce novel chemical properties into a…”
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Evidence for Specific Solvation of Two Halocarbene Amides by Tippmann, Eric M, Platz, Matthew S, Svir, Irina B, Klymenko, Oleksiy V

“…Laser flash photolysis (LFP, 308 nm) of endo-10-halo-10‘-N,N-dimethylcarboxamidetricyclo[4.3.1.0]-deca-2,4-diene (1Cl and 1F) releases indan and halocarbene…”
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Laser Flash Photolysis Study of Chlorofluorocarbene by Tippmann, Eric M, Platz, Matthew S

“…10-exo-Fluoro-10‘-endo-chlorotricylco[4.3.1.0 , ]decadiene-2,4 (1) was synthesized. Continuous photolysis of 1 (300 nm) generates chlorofluorocarbene 2, which…”
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Generation and Characterization of New Fluoro-Substituted Carbenes by Buron, Christophe, Tippmann, Eric M, Platz, Matthew S

“…Singlet fluorocarbenes substituted with diphenylphosphanyl, phenylsulfanyl, and trimethylsilyl groups were generated upon photolysis of the corresponding…”
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