Bioaccumulation of 137Cs and 60Co by bacteria isolated from spent nuclear fuel pools

Bioaccumulation of 137Cs and 60Co by bacteria isolated from spent nuclear fuel pools

This paper is focused on a characterization of bacterial contamination in pool water of the interim spent fuel storage (JAVYS Inc.) in Slovak Republic and on bioaccumulation of 137 Cs and 60 Co by isolated bacteria. Bacterial community in pool water is kept on very low level by extremely low concent...

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Bibliographic Details
Published in:Journal of radioanalytical and nuclear chemistry Vol. 295; no. 1; pp. 737 - 748
Main Authors: Tiookovo, L, Pipioka, M, Godony, A, Hornik, M, Vidovo, B, Augustin, J
Format: Journal Article
Language:English
Published: Dordrecht Springer Netherlands 2013
Subjects:
Bacteria
Bioaccumulation
Chemistry
Chemistry and Materials Science
Cultivation
Diagnostic Radiology
Hadrons
Heavy Ions
Inorganic Chemistry
Nuclear Chemistry
Nuclear Physics
Physical Chemistry
Pools
Pseudomonas aeruginosa
Radioactivity
Spent nuclear fuels
Uptakes
Walls
Cs
Bacteria
Spent nuclear fuel
Co
Bioaccumulation
Storage pools
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Summary:This paper is focused on a characterization of bacterial contamination in pool water of the interim spent fuel storage (JAVYS Inc.) in Slovak Republic and on bioaccumulation of 137 Cs and 60 Co by isolated bacteria. Bacterial community in pool water is kept on very low level by extremely low concentration of solutes in deionized water and by the efficient water filtration system. Based on standard methods and sequencing of 16S rDNA four pure bacterial cultures were identified as Kocuria palustris , Micrococcus luteus , Ochrobactrum spp. and Pseudomonas aeruginosa . Isolated aerobic bacteria were able to bioaccumulate 137 Cs and 60 Co in laboratory experiments. The mechanism of Co and Cs binding involve rapid interactions with anionic groups of the components of cell surface and in the case of Cs + ions is followed by transport processes across cytoplasm membranes and by intracellular distribution. The maximum specific uptake of Cs + after 48 h cultivation in mineral medium (MM) reached 7.54 ± 0.48 μmol g −1  dw ( Ochrobactrum spp.), 19.6 ± 0.1 μmol g −1  dw ( M. luteus ) and 20.1 ± 2.2 μmol g −1  dw ( K. palustris ). The maximum specific uptake of Co 2+ after 24 h cultivation in MM reached 31.1 ± 3.5 μmol g −1 dw ( Ochrobactrum spp.), 86.6 ± 12.2 μmol g −1  dw ( M. luteus ) and 16.9 ± 1.2 μmol g −1 dw ( K. palustris ). These results suggest that due to the long lasting uptake of 137 Cs, 60 Co and other radionuclides by biofilm in pool water high specific radioactivities (Bq m −2 ) can be expected on stainless steel walls of pools.
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ISSN:0236-5731
1588-2780
DOI:10.1007/s10967-012-1932-6