Development and potential use of an Edwardsiella ictaluri wzz mutant as a live attenuated vaccine against enteric septicemia in Pangasius hypophthalmus (Tra catfish)

Development and potential use of an Edwardsiella ictaluri wzz mutant as a live attenuated vaccine against enteric septicemia in Pangasius hypophthalmus (Tra catfish)

Edwardsiella ictaluri is a causative agent of enteric septicemia of catfish (ESC), a seriously lethal disease in Vietnamese catfish (Pangasius hypophthalmus). A safe and effective vaccine against ESC is currently an urgent demand due to antibiotic overuse in pangasius farms has led to an alarming an...

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Published in:Fish & shellfish immunology Vol. 87; pp. 87 - 95
Main Authors: Triet, Tran H., Tinh, Bui T.T., Hau, Le V., Huong, Tran V., Binh, Nguyen-Quoc
Format: Journal Article
Language:English
Published: England Elsevier Ltd 01-04-2019
Subjects:
Edwardsiella ictaluri
Enteric septicemia of catfish
Immersion vaccination
Live attenuated vaccines
wzz gene
Immersion vaccination
wzz gene
Enteric septicemia of catfish
Live attenuated vaccines
Edwardsiella ictaluri
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Summary:Edwardsiella ictaluri is a causative agent of enteric septicemia of catfish (ESC), a seriously lethal disease in Vietnamese catfish (Pangasius hypophthalmus). A safe and effective vaccine against ESC is currently an urgent demand due to antibiotic overuse in pangasius farms has led to an alarming antimicrobial resistance. In this study, two E. ictaluri wzzE mutants (WzM-L3, deficient in a 1038bp-entire wzzE gene and WzM-S3, a 245bp-partial deletion of wzzE) were developed and their protection efficiacy was evaluated in hatched pangasius against ESC by immersion vaccination. As comparing to the high virulent wild-type strain who caused 73.33% of death on pangasius fingerlings immersed at 7.1 × 106 CFU ml−1, both mutants showed extremely low mortality rates at 3.33% (WzM-S3) and 0% (WzM-L3) on pangasius fingerlings immersed at high concentration of 1.5 × 107 CFU mL−1 and 9.7 × 106 CFU ml−1, respectively. Interestingly, both WzM-S3 and WzM-L3 had a remarkably high protection against ESC, as RPS % were found at 89.29% and 90%, respectively. The mutant WzM-L3 is a potential live attenuated vaccine against ESC in Vietnamese catfish farms with good protection and simple practice. •ECA chain length is modulated by WzzE, a vital scaffold protein in ECA biosynthesis pathway.•WzzE, encoded by wzzE in wec cluster, is conserved and prevalent among Enterobacteria.•Mutant ΔwzzE of E. ictaluri showed attenuated and effective in protecting pangasius against ESC disease by immersion
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ISSN:1050-4648
1095-9947
DOI:10.1016/j.fsi.2019.01.005