Search Results - "Ting, Wan‐Wen"

Effective 5‐aminolevulinic acid production via T7 RNA polymerase and RuBisCO equipped Escherichia coli W3110 by Ting, Wan‐Wen, Ng, I‐Son

“…Chromosome‐based engineering is a superior approach for gene integration generating a stable and robust chassis. Therefore, an effective amplifier, T7 RNA…”
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Metabolic manipulation through CRISPRi and gene deletion to enhance cadaverine production in Escherichia coli by Ting, Wan-Wen, Ng, I-Son

“…Due to the limiting natural resources, greenhouse effect and global warming crisis, the bio-based chemicals which are environmentally friendly materials have…”
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Genome‐wide survey of open chromatin regions in two swallowtail butterflies Papilio machaon and P. bianor by Wan, Wen‐ting, Hu, Ping, Chang, Zhou, Ren, Yan‐dong, Dong, Zhi‐wei, Yang, Jie, Pan, Xiang‐yu, He, Jin‐wu, Liu, Wei, Liu, Gui‐chun, Zhao, Ruo‐ping, Mao, Chu‐yang, Li, Jun, Wang, Wen, Li, Xue‐yan

“…Papilio machaon was assigned as the type species for all butterflies by Linnaeus and P. bianor is a congener but exhibits a great difference in morphology…”
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Integrated Analysis of Transcriptome and Proteome to Reveal Pupal Color Switch in Papilio xuthus Butterflies by He, Jin-Wu, Dong, Zhi-Wei, Hu, Ping, Liu, Wei, Zhang, Ru, Liu, Gui-Chun, Zhao, Ruo-Ping, Wan, Wen-Ting, Wang, Wen, Li, Xue-Yan

“…Pupal color polyphenism in butterflies, including green, intermediate, or brown, is an excellent study system for understanding phenotypic plasticity. Previous…”
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Development of chromosome-based T7 RNA polymerase and orthogonal T7 promoter circuit in Escherichia coli W3110 as a cell factory by Ting, Wan-Wen, Tan, Shih-I, Ng, I-Son

“…Background Orthogonal T7 RNA polymerase (T7RNAP) and T7 promoter is a powerful genetic element to mediate protein expression in different cells. Among all,…”
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Enhanced recombinant carbonic anhydrase in T7RNAP-equipped Escherichia coli W3110 for carbon capture storage and utilization (CCSU) by Ting, Wan-Wen, Yu, Jie-Yao, Lin, Yu-Chieh, Ng, I-Son

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Genetic design of co-expressed Mesorhizobium loti carbonic anhydrase and chaperone GroELS to enhancing carbon dioxide sequestration by Effendi, Sefli Sri Wahyu, Tan, Shih-I, Ting, Wan-Wen, Ng, I-Son

“…Mesorhizobium loti carbonic anhydrase (MlCA), an intrinsically high catalytic enzyme, has been employed for carbon dioxide capture and sequestration. However,…”
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Enhanced recombinant Sulfurihydrogenibium yellowstonense carbonic anhydrase activity and thermostability by chaperone GroELS for carbon dioxide biomineralization by Wahyu Effendi, Sefli Sri, Tan, Shih-I, Ting, Wan-Wen, Ng, I-Son

“…Biological carbon fixation is a feasible strategy to reduce atmospheric carbon dioxide levels (CO2). In this platform, carbonic anhydrase (CA) enzyme is…”
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A novel toggle switch under acid-base regulation for tunable gamma-aminobutyric acid (GABA) production in Escherichia coli by Xue, Chengfeng, Ting, Wan-Wen, Ng, I-Son

“…•A novel toggle switch between glutamate decarboxylase and tryptophanase has been revealed.•GABA inhibited tryptophanase activity only under acidic conditions…”
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Development of Escherichia coli Nissle 1917 derivative by CRISPR/Cas9 and application for gamma-aminobutyric acid (GABA) production in antibiotic-free system by Lan, Yi-Jun, Tan, Shih-I, Cheng, Shu-Yun, Ting, Wan-Wen, Xue, Chengfeng, Lin, Tzu-Han, Cai, Ming-Zhi, Chen, Po-Ting, Ng, I-Son

“…•Plasmid-free E. coli Nissle (EcNP) was successfully constructed via CRISPR/Cas9.•Multiple DNA cleavage on plasmid enhances the efficiency of plasmid…”
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Tunable T7 Promoter Orthogonality on T7RNAP for cis-Aconitate Decarboxylase Evolution via Base Editor and Screening from Itaconic Acid Biosensor by Ting, Wan-Wen, Ng, I-Son

“…The deaminase-fused T7 RNA polymerase (T7RNAP) presents a promising toolkit for in vivo target-specific enzyme evolution, offering the unique advantage of…”
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Exploring PETase-like enzyme from shotgun metagenome and co-expressing Colicin E7 in Escherichia coli for effective PET degradation by Effendi, Sefli Sri Wahyu, Hu, Ruei-En, Hsiang, Chuan-Chieh, Ting, Wan-Wen, Huang, Chao-Li, Ng, I-Son

“…Polyethylene terephthalate (PET) is the conventional plastic used for packaging but disposal of PET in the natural environment poses significant threats to the…”
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Whole-cell biocatalyst for cadaverine production using stable, constitutive and high expression of lysine decarboxylase in recombinant Escherichia coli W3110 by Ting, Wan-Wen, Huang, Chih-Yu, Wu, Pong-Yee, Huang, Shih-Fang, Lin, Hung-Yi, Li, Sheng-Feng, Chang, Jo-Shu, Ng, I-Son

“…•J23100 promoter was suitable for lysine decarboxylase expression in E. coli W3110.•Strain JW showed the excellent plasmid stability even in antibiotic free…”
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New insight into the codon usage and medium optimization toward stable and high-level 5-aminolevulinic acid production in Escherichia coli by Yu, Tzu-Hsuan, Tan, Shih-I, Yi, Ying-Chen, Xue, Chengfeng, Ting, Wan-Wen, Chang, Jui-Jen, Ng, I-Son

“…5–Aminolevulinic acid (5-ALA) is recently being used as a critical prodrug used for the photodynamic therapy of cancers. In this study, we first demonstrated…”
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Heterologous expression and characterization of Aquabacterium parvum lipase, a close relative of Ideonella sakaiensis PETase in Escherichia coli by Ho, Ngai Hei Ernest, Effendi, Sefli Sri Wahyu, Ting, Wan-Wen, Yi, Ying-Chen, Yu, Jie-Yao, Chang, Jo-Shu, Ng, I-Son

“…Polyethylene terephthalate (PET) is a globally mismanaged plastic waste. Microbial degradation of plastics is a green and sustainable technology and very few…”
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Directed evolution of Mesorhizobium loti carbonic anhydrase for carbon dioxide sequestration by MutaT7 and rational codon design by Ting, Wan-Wen, Effendi, Sefli Sri Wahyu, Hu, Ruei-En, Ng, I-Son

“…•A chromosomal dCas9 with CAPsg as DEPEND-2.0 effectively repressed initial cell growth.•115 colonies were screened on plate through two rounds of MlCA…”
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Efficient biotransformation of l-lysine into cadaverine by strengthening pyridoxal 5’-phosphate-dependent proteins in Escherichia coli with cold shock treatment by Xue, Chengfeng, Hsu, Kai-Min, Ting, Wan-Wen, Huang, Shih-Fang, Lin, Hung-Yi, Li, Sheng-Feng, Chang, Jo-Shu, Ng, I-Son

“…[Display omitted] •BL21 harboring pdxY under the J23100 promoter resulted in highest yield of PLP.•pdxK plays a critical role in accumulation of PLP for…”
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Precise measurement of decarboxylase and applied cascade enzyme for simultaneous cadaverine production with carbon dioxide recovery by Lin, Yu-Chieh, Xue, Chengfeng, Tan, Shih-I, Ting, Wan-Wen, Yang, Shih-Chen, Ng, I-Son

“…•Feasible and precise quantification of decarboxylases activity has been demonstrated.•Effective sequestration of CO2 from decarboxylation successfully used…”
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Facilitating the enzymatic conversion of lysineto cadaverine in engineered Escherichia coli with metabolic regulation by genes deletion by Huang, Chih-Yu, Ting, Wan-Wen, Chen, Ying-Chun, Wu, Pong-Yee, Dong, Cheng-Di, Huang, Shih-Fang, Lin, Hung-Yi, Li, Sheng-Feng, Ng, I-Son, Chang, Jo-Shu

“…[Display omitted] •Polyamine metabolic genes knockout strain was used for cadaverine production.•Lysine utilization efficiency is improved in the polyamine…”
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Synthesis and in vitro study of pseudo-peptide thioureas containing α-aminophosphonate moiety as potential antitumor agents by Liu, Jing-Zi, Song, Bao-An, Fan, Hui-Tao, Bhadury, Pinaki S., Wan, Wen-Ting, Yang, Song, Xu, Weiming, Wu, Jian, Jin, Lin-Hong, Wei, Xue, Hu, De-Yu, Zeng, Song

“…Twenty pseudo-peptide thioureas IIa–l containing α-aminophosphonate moiety were synthesized from the reaction of chiral α-amino carboxamide derivatives Ia–c…”
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