determination and comparison of the 16S rRNA gene sequences of species of the genus Pseudomonas (sensu stricto) and estimation of the natural intrageneric relationships

As a consolidated effort on the part of several laboratories, partial and nearly complete sequence determinations of 16S rRNA genes have been applied as one of several analytical methods in a polyphasic study of the pseudomonads. Nearly-complete sequences have been determined of the PCR-amplified 16...

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Bibliographic Details
Published in:	Systematic and applied microbiology Vol. 19; no. 4; pp. 478 - 492
Main Authors:	Moore, E.R.B, Mau, M, Arnscheidt, A, Bottger, E.C, Hutson, R.A, Collins, M.D, Peer, Y. van de, Wachter, R. de, Timms, K.N
Format:	Journal Article
Language:	English
Published:	1996
Subjects:	comparisons identification molecular sequence data nucleotide sequences phylogeny Pseudomonas ribosomal RNA
Online Access:	Get full text
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Summary:	As a consolidated effort on the part of several laboratories, partial and nearly complete sequence determinations of 16S rRNA genes have been applied as one of several analytical methods in a polyphasic study of the pseudomonads. Nearly-complete sequences have been determined of the PCR-amplified 16S rRNA genes of 21 species of the genus Pseudomonas (sensu stricto), including multiple strains of most species. Phylogenetic branching orders and the natural intrageneric relationships among the species have been inferred through sequence comparisons and cluster analysis and have not shown any obvious recognizable correlation with results derived through standard phenotypic criteria commonly used to group the species. This paper also focuses on the ability of 16S rRNA gene sequences, particularly the hypervariable sequence regions, to be used as nested identification markers and as target sites for the development of 16S rRNA sequence-based strategies for the identification of species of the genus Pseudomonas.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0723-2020 1618-0984
DOI:	10.1016/S0723-2020(96)80021-X