Nucleolar association of pEg7 and XCAP-E, two members of Xenopus laevis condensin complex in interphase cells

Cell cycle dynamics and localization of condensins--multiprotein complexes involved in late stages of mitotic chromosome condensation--were studied in Xenopus laevis XL2 cell line. Western blot analysis of synchronized cells showed that the ratio of levels of both pEg7 and XCAP-E to beta-tubulin lev...

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Bibliographic Details
Published in:	Journal of cell science Vol. 116; no. Pt 9; pp. 1667 - 1678
Main Authors:	Uzbekov, Rustem, Timirbulatova, Elmira, Watrin, Erwan, Cubizolles, Fabien, Ogereau, David, Gulak, Pavel, Legagneux, Vincent, Polyakov, Vladimir Ju, Le Guellec, Katherine, Kireev, Igor
Format:	Journal Article
Language:	English
Published:	England Company of Biologists 01-05-2003
Subjects:	Adenosine Triphosphatases Adenosine Triphosphatases - chemistry Adenosine Triphosphatases - metabolism Animals Biochemistry, Molecular Biology Carrier Proteins Carrier Proteins - chemistry Carrier Proteins - metabolism Cell Cycle Cell Cycle - physiology Cell Cycle Proteins Cell Cycle Proteins - chemistry Cell Cycle Proteins - metabolism Cell Line Cell Nucleolus Cell Nucleolus - metabolism Chromosomes Chromosomes - metabolism DNA-Binding Proteins DNA-Binding Proteins - chemistry DNA-Binding Proteins - metabolism Egg Proteins Egg Proteins - chemistry Egg Proteins - metabolism Genomics Interphase Interphase - physiology Life Sciences Macromolecular Substances Microscopy, Immunoelectron Multiprotein Complexes Nuclear Proteins Nuclear Proteins - chemistry Nuclear Proteins - metabolism RNA, Ribosomal RNA, Ribosomal - genetics RNA, Ribosomal - metabolism Transcription, Genetic Xenopus laevis Xenopus Proteins
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Summary:	Cell cycle dynamics and localization of condensins--multiprotein complexes involved in late stages of mitotic chromosome condensation--were studied in Xenopus laevis XL2 cell line. Western blot analysis of synchronized cells showed that the ratio of levels of both pEg7 and XCAP-E to beta-tubulin levels remains almost constant from G1 to M phase. pEg7 and XCAP-E were localized to the mitotic chromosomes and were detected in interphase nuclei. Immunostaining for condensins and nucleolar proteins UBF, fibrillarin and B23 revealed that both XCAP-E and pEg7 are localized in the granular component of the nucleolus. Nucleolar labeling of both proteins is preserved in segregated nucleoli after 6 hours of incubation with actinomycin D (5 mg/ml), but the size of the labeled zone was significantly smaller. The data suggest a novel interphase function of condensin subunits in spatial organization of the nucleolus and/or ribosome biogenesis.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	0021-9533 1477-9137
DOI:	10.1242/jcs.00311