Search Results - "Thyer, Ross"

The Role of tRNA in Establishing New Genetic Codes by Thyer, Ross, Ellington, Andrew D

“…One of the most remarkable, but typically unremarked, aspects of the translation apparatus is the pleiotropic pliability of tRNA. This humble…”
Get full text

Discovery of Novel Gain-of-Function Mutations Guided by Structure-Based Deep Learning by Shroff, Raghav, Cole, Austin W, Diaz, Daniel J, Morrow, Barrett R, Donnell, Isaac, Annapareddy, Ankur, Gollihar, Jimmy, Ellington, Andrew D, Thyer, Ross

“…Despite the promise of deep learning accelerated protein engineering, examples of such improved proteins are scarce. Here we report that a 3D convolutional…”
Get full text

Engineered rRNA Enhances the Efficiency of Selenocysteine Incorporation during Translation by Thyer, Ross, Filipovska, Aleksandra, Rackham, Oliver

“…We developed a new genetic selection approach to screen for mutations that can alter the efficiency of selenocysteine incorporation. We identified mutations in…”
Get full text

Addicting diverse bacteria to a noncanonical amino acid by Tack, Drew S, Ellefson, Jared W, Thyer, Ross, Wang, Bo, Gollihar, Jimmy, Forster, Matthew T, Ellington, Andrew D

“…Expansion of the genetic code to noncanonical amino acids (NCAAs) has been limited by the lack of evolutionary pressure for organismal dependence on the NCAA…”
Get full text

Substrate and inhibitor specificities differ between human cytosolic and mitochondrial thioredoxin reductases: Implications for development of specific inhibitors by Rackham, Oliver, Shearwood, Anne-Marie J., Thyer, Ross, McNamara, Elyshia, Davies, Stefan M.K., Callus, Bernard A., Miranda-Vizuete, Antonio, Berners-Price, Susan J., Cheng, Qing, Arnér, Elias S.J., Filipovska, Aleksandra

“…The cytosolic and mitochondrial thioredoxin reductases (TrxR1 and TrxR2) and thioredoxins (Trx1 and Trx2) are key components of the mammalian thioredoxin…”
Get full text

Evolving tRNASec for Efficient Canonical Incorporation of Selenocysteine by Thyer, Ross, Robotham, Scott A, Brodbelt, Jennifer S, Ellington, Andrew D

“…Bacterial selenocysteine incorporation occurs in response to opal stop codons and is dependent on the presence of a selenocysteine insertion sequence (SECIS)…”
Get full text

DLPacker: Deep learning for prediction of amino acid side chain conformations in proteins by Misiura, Mikita, Shroff, Raghav, Thyer, Ross, Kolomeisky, Anatoly B.

“…Prediction of side chain conformations of amino acids in proteins (also termed “packing”) is an important and challenging part of protein structure prediction…”
Get full text

Synthetic biology: New letters for life's alphabet by Thyer, Ross, Ellefson, Jared

Get full text

New letters for life's alphabet by Thyer, Ross, Ellefson, Jared

“…The five bases found in nucleic acids define the 'alphabet' used to encode life on Earth. The construction of an organism that stably propagates an unnatural…”
Get full text

Integrated Top-Down and Bottom-Up Mass Spectrometry for Characterization of Diselenide Bridging Patterns of Synthetic Selenoproteins by Watts, Eleanor, Thyer, Ross, Ellington, Andrew D., Brodbelt, Jennifer S.

“…With the rapid acceleration in the design and development of new biotherapeutics, ensuring consistent quality and understanding degradation pathways remain…”
Get full text

Using fungible biosensors to evolve improved alkaloid biosyntheses by d’Oelsnitz, Simon, Kim, Wantae, Burkholder, Nathaniel T., Javanmardi, Kamyab, Thyer, Ross, Zhang, Yan, Alper, Hal S., Ellington, Andrew D.

“…A key bottleneck in the microbial production of therapeutic plant metabolites is identifying enzymes that can improve yield. The facile identification of…”
Get full text

Structural Characterization of Dihydrofolate Reductase Complexes by Top-Down Ultraviolet Photodissociation Mass Spectrometry by Cammarata, Michael B, Thyer, Ross, Rosenberg, Jake, Ellington, Andrew, Brodbelt, Jennifer S

“…The stepwise reduction of dihydrofolate to tetrahydrofolate entails significant conformational changes of dihydrofolate reductase (DHFR). Binary and ternary…”
Get full text

Evolving tRNA(Sec) for efficient canonical incorporation of selenocysteine by Thyer, Ross, Robotham, Scott A, Brodbelt, Jennifer S, Ellington, Andrew D

“…Bacterial selenocysteine incorporation occurs in response to opal stop codons and is dependent on the presence of a selenocysteine insertion sequence (SECIS)…”
Get full text

Directed Evolution of an Improved Aminoacyl‐tRNA Synthetase for Incorporation of L‐3,4‐Dihydroxyphenylalanine (L‐DOPA) by Thyer, Ross, d'Oelsnitz, Simon, Blevins, Molly S., Klein, Dustin R., Brodbelt, Jennifer S., Ellington, Andrew D.

“…The catechol group of 3,4‐dihydroxyphenylalanine (L‐DOPA) derived from L‐tyrosine oxidation is a key post‐translational modification (PTM) in many protein…”
Get full text

A modular toolkit for environmental Rhodococcus , Gordonia , and Nocardia enables complex metabolic manipulation by Jansen, Zachary, Alameri, Abdulaziz, Wei, Qiyao, Kulhanek, Devon L, Gilmour, Andrew R, Halper, Sean, Schwalm, 3rd, Nathan D, Thyer, Ross

“…Soil-dwelling Actinomycetes are a diverse and ubiquitous component of the global microbiome but largely lack genetic tools comparable to those available in…”
Get more information

Characterization of trimethoprim resistant E. coli dihydrofolate reductase mutants by mass spectrometry and inhibition by propargyl-linked antifolates by Cammarata, Michael, Thyer, Ross, Lombardo, Michael, Anderson, Amy, Wright, Dennis, Ellington, Andrew, Brodbelt, Jennifer S

“…Pathogenic , one of the primary causes of urinary tract infections, has shown significant resistance to the most popular antibiotic, trimethoprim (TMP), which…”
Get full text

Custom selenoprotein production enabled by laboratory evolution of recoded bacterial strains by Thyer, Ross, Shroff, Raghav, Klein, Dustin R, d'Oelsnitz, Simon, Cotham, Victoria C, Byrom, Michelle, Brodbelt, Jennifer S, Ellington, Andrew D

“…Selenocysteine is efficiently incorporated into recombinant proteins using evolved Escherichia coli strains. Incorporation of the rare amino acid…”
Get full text

How to Balance the Many Roles of tRNAs During the Creation of New Genetic Codes by Ellington, Andrew, Maranhao, Andre, Baldridge, Kevin, Tack, Drew, Thyer, Ross, Contreras, Lydia

“…Transfer RNA is a remarkable molecule, in that it sits at the crossroads between many competing functions, including charging, loading onto the ribosome,…”
Get full text

Interrogating the Function of Bicistronic Translational Control Elements to Improve Consistency of Gene Expression by Jansen, Zachary, Reilly, Sophia R., Lieber-Kotz, Matan, Li, Andrew Z., Wei, Qiyao, Kulhanek, Devon L., Gilmour, Andrew R., Thyer, Ross

“…Context independent gene expression is required for genetic circuits to maintain consistent and predicable behavior. Previous efforts to develop context…”
Get full text

Characterization of trimethoprim resistant E. coli dihydrofolate reductase mutants by mass spectrometry and inhibition by propargyl-linked antifolatesElectronic supplementary information (ESI) available: Supplemental Fig. 1-7. These figures include the sequence of DHFR, typical MS1 spectra of DHFR complexes, SEC elution profiles of DHF, UVPD mass spectra of P21L·NADPH complexes and sequence maps, difference plots of UVPD backbone cleavage propensities for binary and ternary complexes, and cell g by Cammarata, Michael, Thyer, Ross, Lombardo, Michael, Anderson, Amy, Wright, Dennis, Ellington, Andrew, Brodbelt, Jennifer S

“…Pathogenic Escherichia coli , one of the primary causes of urinary tract infections, has shown significant resistance to the most popular antibiotic,…”
Get full text