Search Results - "Thuenemann, Eva C"
-
1
pEAQ: versatile expression vectors for easy and quick transient expression of heterologous proteins in plants
Published in Plant biotechnology journal (01-09-2009)“…Agro-infiltration of leaf tissue with binary vectors harbouring a sequence of interest is a rapid method of expressing proteins in plants. It has recently been…”
Get more information
Journal Article -
2
A method for rapid production of heteromultimeric protein complexes in plants: assembly of protective bluetongue virus-like particles
Published in Plant biotechnology journal (01-09-2013)“…Plant expression systems based on nonreplicating virus-based vectors can be used for the simultaneous expression of multiple genes within the same cell. They…”
Get more information
Journal Article -
3
Tandem fusion of hepatitis B core antigen allows assembly of virus-like particles in bacteria and plants with enhanced capacity to accommodate foreign proteins
Published in PloS one (01-04-2015)“…The core protein of the hepatitis B virus, HBcAg, assembles into highly immunogenic virus-like particles (HBc VLPs) when expressed in a variety of heterologous…”
Get full text
Journal Article -
4
Producing Vaccines against Enveloped Viruses in Plants: Making the Impossible, Difficult
Published in Vaccines (Basel) (13-07-2021)“…The past 30 years have seen the growth of plant molecular farming as an approach to the production of recombinant proteins for pharmaceutical and…”
Get full text
Journal Article -
5
A Replicating Viral Vector Greatly Enhances Accumulation of Helical Virus-Like Particles in Plants
Published in Viruses (11-05-2021)“…The production of plant helical virus-like particles (VLPs) via plant-based expression has been problematic with previous studies suggesting that an RNA…”
Get full text
Journal Article -
6
CryoEM and stability analysis of virus-like particles of potyvirus and ipomovirus infecting a common host
Published in Communications biology (19-04-2023)“…Sweet potato feathery mottle virus (SPFMV) and Sweet potato mild mottle virus (SPMMV) are members of the genera Potyvirus and Ipomovirus , family Potyviridae ,…”
Get full text
Journal Article -
7
The use of transient expression systems for the rapid production of virus-like particles in plants
Published in Current pharmaceutical design (01-09-2013)“…Advances in transient expression technologies have allowed the production of milligram quantities of proteins within a matter of days using only small amounts…”
Get more information
Journal Article -
8
The Use of a Replicating Virus Vector For in Planta Generation of Tobacco Mosaic Virus Nanorods Suitable For Metallization
Published in Frontiers in bioengineering and biotechnology (26-04-2022)“…The production of designer-length tobacco mosaic virus (TMV) nanorods in plants has been problematic in terms of yields, particularly when modified coat…”
Get full text
Journal Article -
9
The Tobacco Mosaic Virus Origin of Assembly Sequence is Dispensable for Specific Viral RNA Encapsidation but Necessary for Initiating Assembly at a Single Site
Published in Journal of molecular biology (30-12-2022)“…[Display omitted] •The role of the tobacco mosaic virus origin of assembly sequence was reinvestigated.•For replicating RNA, the TMV OAS is not required for…”
Get full text
Journal Article -
10
Recent advances of Cowpea mosaic virus-based particle technology
Published in Human vaccines (01-03-2011)“…Particles of cowpea mosaic virus (CPMV) have enjoyed considerable success as a means of presenting peptides for vaccine purposes. However, the existing…”
Get full text
Journal Article -
11
Designer-length palladium nanowires can be templated by the central channel of tobacco mosaic virus nanorods
Published in Virology (New York, N.Y.) (01-12-2022)“…We have developed methods for the templated synthesis of palladium nanowires (Pd NWs) within the central channel of tobacco mosaic virus (TMV) nanorods of…”
Get full text
Journal Article -
12
miRNAs control gene expression in the single-cell alga Chlamydomonas reinhardtii
Published in Nature (28-06-2007)“…MicroRNAs (miRNAs) in eukaryotes guide post-transcriptional regulation by means of targeted RNA degradation and translational arrest. They are released by a…”
Get full text
Journal Article -
13
Plant‐made dengue virus‐like particles produced by co‐expression of structural and non‐structural proteins induce a humoral immune response in mice
Published in Plant biotechnology journal (01-04-2021)“…Summary Dengue virus (DENV) is an emerging threat causing an estimated 390 million infections per year. Dengvaxia, the only licensed vaccine, may not be…”
Get full text
Journal Article -
14
Viral nanoparticles can elude protein barriers: exploiting rather than imitating nature
Published in Nanoscale (31-01-2019)“…Protein-corona formation in body fluids and/or entrapment of nanoparticles in protein matrices (e.g. food and mucus) can hinder the delivery of nanoparticles,…”
Get more information
Journal Article -
15
Virus-Derived Vectors for the Expression of Multiple Proteins in Plants
Published in Methods in molecular biology (Clifton, N.J.) (01-01-2016)“…This chapter constitutes a practical guide to using the "pEAQ" vector series for transient or stable expression of one or more protein(s) in Nicotiana…”
Get more information
Journal Article -
16
Engineering Recombinant Virus-like Nanoparticles from Plants for Cellular Delivery
Published in ACS nano (25-04-2017)“…Understanding capsid assembly following recombinant expression of viral structural proteins is critical to the design and modification of virus-like…”
Get full text
Journal Article -
17
Bluetongue Virus Particles as Nanoreactors for Enzyme Delivery and Cancer Therapy
Published in Molecular pharmaceutics (01-03-2021)“…The side effects of chemotherapy can be reduced by targeting tumor cells with an enzyme (or the corresponding gene) that converts a nontoxic prodrug into a…”
Get full text
Journal Article -
18
Delivering Cargo: Plant-Based Production of Bluetongue Virus Core-Like and Virus-Like Particles Containing Fluorescent Proteins
Published in Methods in molecular biology (Clifton, N.J.) (2018)“…This chapter provides a practical guide to the in planta transient production of bluetongue virus-like particles containing a fluorescent cargo protein…”
Get more information
Journal Article -
19
Tandem Fusion of Hepatitis B Core Antigen Allows Assembly of Virus-Like Particles in Bacteria and Plants with Enhanced Capacity to Accommodate Foreign Proteins: e0120751
Published in PloS one (01-04-2015)“…The core protein of the hepatitis B virus, HBcAg, assembles into highly immunogenic virus-like particles (HBc VLPs) when expressed in a variety of heterologous…”
Get full text
Journal Article