Search Results - "Thotsaporn, Kittisak"

Kinetic Mechanism of the Dechlorinating Flavin-dependent Monooxygenase HadA by Pimviriyakul, Panu, Thotsaporn, Kittisak, Sucharitakul, Jeerus, Chaiyen, Pimchai

“…The accumulation of chlorophenols (CPs) in the environment, due to their wide use as agrochemicals, has become a serious environmental problem. These organic…”
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Stabilization of C4a-Hydroperoxyflavin in a Two-component Flavin-dependent Monooxygenase Is Achieved through Interactions at Flavin N5 and C4a Atoms by Thotsaporn, Kittisak, Chenprakhon, Pirom, Sucharitakul, Jeerus, Mattevi, Andrea, Chaiyen, Pimchai

“…p-Hydroxyphenylacetate (HPA) 3-hydroxylase is a two-component flavin-dependent monooxygenase. Based on the crystal structure of the oxygenase component (C2),…”
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Control of C4a-Hydroperoxyflavin Protonation in the Oxygenase Component of p‑Hydroxyphenylacetate-3-hydroxylase by Chenprakhon, Pirom, Trisrivirat, Duangthip, Thotsaporn, Kittisak, Sucharitakul, Jeerus, Chaiyen, Pimchai

“…The protonation status of the peroxide moiety in C4a-(hydro)peroxyflavin of p-hydroxyphenylacetate-3-hydroxylase can be directly monitored using transient…”
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Multiple pathways guide oxygen diffusion into flavoenzyme active sites by Baron, Riccardo, Riley, Conor, Chenprakhon, Pirom, Thotsaporn, Kittisak, Winter, Remko T, Alfieri, Andrea, Forneris, Federico, van Berkel, Willem J.H, Chaiyen, Pimchai, Fraaije, Marco W, Mattevi, Andrea, McCammon, J. Andrew

“…Dioxygen (O₂) and other gas molecules have a fundamental role in a variety of enzymatic reactions. However, it is only poorly understood which O₂ uptake…”
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Effectiveness of iodine for continuous decontamination of dental unit waterline by Petchphayaprai, Chatchaya, Chotipan, Chutimon, Sa-ngiampak, Pitchayapa, Thotsaporn, Kittisak, Ampornaramveth, Ruchanee Salingcarnboriboon

“…Objective Dental Unit Waterlines (DUWLs) are contaminated by various species of microorganisms. DUWLs should be disinfected appropriately to control microbial…”
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3,4-Dihydroxyphenylacetate 2,3-dioxygenase from Pseudomonas aeruginosa: An Fe(II)-containing enzyme with fast turnover by Pornsuwan, Soraya, Maenpuen, Somchart, Kamutira, Philaiwarong, Watthaisong, Pratchaya, Thotsaporn, Kittisak, Tongsook, Chanakan, Juttulapa, Maneerat, Nijvipakul, Sarayut, Chaiyen, Pimchai

“…3,4-dihydroxyphenylacetate (DHPA) dioxygenase (DHPAO) from Pseudomonas aeruginosa (PaDHPAO) was overexpressed in Escherichia coli and purified to homogeneity…”
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Cloning and expression of p-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii: evidence of the divergence of enzymes in the class of two-protein component aromatic hydroxylases by Thotsaporn, Kittisak, Sucharitakul, Jeerus, Wongratana, Janewit, Suadee, Chutintorn, Chaiyen, Pimchai

“…The genes encoding for the reductase and oxygenase components of p-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii were cloned and expressed in…”
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Enzymes in the p-hydroxyphenylacetate degradation pathway of Acinetobacter baumannii by Thotsaporn, Kittisak, Tinikul, Ruchanok, Maenpuen, Somchart, Phonbuppha, Jittima, Watthaisong, Pratchaya, Chenprakhon, Pirom, Chaiyen, Pimchai

“…[Display omitted] •This report is the characterization of p-hydroxyphenylacetate (HPA) degradation operon in Acinetobacter baumannii.•The gene arrangement of…”
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The fusion Vibrio campbellii luciferase as a eukaryotic gene reporter by Tinikul, Ruchanok, Thotsaporn, Kittisak, Thaveekarn, Wichit, Jitrapakdee, Sarawut, Chaiyen, Pimchai

“…► The fusion form of Vibrio campbellii luciferase can efficiently catalyze the reaction. ► The fusion luciferase is stable at 37°C and binds tightly to flavin…”
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Interactions with the Substrate Phenolic Group Are Essential for Hydroxylation by the Oxygenase Component of p-Hydroxyphenylacetate 3-Hydroxylase by Tongsook, Chanakan, Sucharitakul, Jeerus, Thotsaporn, Kittisak, Chaiyen, Pimchai

“…p-Hydroxyphenylacetate (HPA) 3-hydroxylase is a two-component flavoprotein monooxygenase that catalyzes the hydroxylation of p-hydroxyphenylacetate to form…”
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3,4-Dihydroxyphenylacetate 2,3-dioxygenase from Pseudomonas aeruginosa: An Fe by Pornsuwan, Soraya, Maenpuen, Somchart, Kamutira, Philaiwarong, Watthaisong, Pratchaya, Thotsaporn, Kittisak, Tongsook, Chanakan, Juttulapa, Maneerat, Nijvipakul, Sarayut, Chaiyen, Pimchai

“…3,4-dihydroxyphenylacetate (DHPA) dioxygenase (DHPAO) from Pseudomonas aeruginosa (PaDHPAO) was overexpressed in Escherichia coli and purified to homogeneity…”
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The C-terminal Domain of 4-Hydroxyphenylacetate 3-Hydroxylase from Acinetobacter baumannii Is an Autoinhibitory Domain by Phongsak, Thanawat, Sucharitakul, Jeerus, Thotsaporn, Kittisak, Oonanant, Worrapoj, Yuvaniyama, Jirundon, Svasti, Jisnuson, Ballou, David P., Chaiyen, Pimchai

“…Background: The flavin reduction rate of C1 reductase is enhanced ∼20-fold upon binding to HPA (effector). Results: The truncated C1 variant lacking the…”
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