Search Results - "Thorne, Robert E."

Quantifying radiation damage in biomolecular small-angle X-ray scattering by Hopkins, Jesse B., Thorne, Robert E.

“…Small‐angle X‐ray scattering (SAXS) is an increasingly popular technique that provides low‐resolution structural information about biological macromolecules in…”
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Hypothesis for a mechanism of beam-induced motion in cryo-electron microscopy by Thorne, Robert E

“…Estimates of heat-transfer rates during plunge-cooling and the patterns of ice observed in cryo-EM samples indicate that the grid bars cool much more slowly…”
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Effects of protein‐crystal hydration and temperature on side‐chain conformational heterogeneity in monoclinic lysozyme crystals by Atakisi, Hakan, Moreau, David W., Thorne, Robert E.

“…The modulation of main‐chain and side‐chain conformational heterogeneity and solvent structure in monoclinic lysozyme crystals by dehydration (related to water…”
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Ice formation and its elimination in cryopreservation of oocytes by Abdelhady, Abdallah W., Mittan-Moreau, David W., Crane, Patrick L., McLeod, Matthew J., Cheong, Soon Hon, Thorne, Robert E.

“…Damage from ice and potential toxicity of ice-inhibiting cryoprotective agents (CPAs) are key issues in assisted reproduction of humans, domestic and research…”
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Breaking the Radiation Damage Limit with Cryo-SAXS by Meisburger, Steve P., Warkentin, Matthew, Chen, Huimin, Hopkins, Jesse B., Gillilan, Richard E., Pollack, Lois, Thorne, Robert E.

“…Small angle x-ray scattering (SAXS) is a versatile and widely used technique for obtaining low-resolution structures of macromolecules and complexes. SAXS…”
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Integrated sample‐handling and mounting system for fixed‐target serial synchrotron crystallography by Illava, Gabrielle, Jayne, Richard, Finke, Aaron D., Closs, David, Zeng, Wenjie, Milano, Shawn K., Huang, Qingqiu, Kriksunov, Irina, Sidorenko, Pavel, Wise, Frank W., Zipfel, Warren R., Apker, Benjamin A., Thorne, Robert E.

“…Serial synchrotron crystallography (SSX) is enabling the efficient use of small crystals for structure–function studies of biomolecules and for drug discovery…”
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Resolution and dose dependence of radiation damage in biomolecular systems by Atakisi, Hakan, Conger, Lauren, Moreau, David W, Thorne, Robert E

“…The local Fourier-space relation between diffracted intensity , diffraction wavevector and dose , , is key to probing and understanding radiation damage by…”
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High-resolution single-particle cryo-EM of samples vitrified in boiling nitrogen by Engstrom, Tyler, Clinger, Jonathan A., Spoth, Katherine A., Clarke, Oliver B., Closs, David S., Jayne, Richard, Apker, Benjamin A., Thorne, Robert E.

“…Based on work by Dubochet and others in the 1980s and 1990s, samples for single-particle cryo-electron microscopy (cryo-EM) have been vitrified using ethane,…”
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Determining biomolecular structures near room temperature using X‐ray crystallography: concepts, methods and future optimization by Thorne, Robert E.

“…For roughly two decades, cryocrystallography has been the overwhelmingly dominant method for determining high‐resolution biomolecular structures. Competition…”
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Millisecond mix-and-quench crystallography (MMQX) enables time-resolved studies of PEPCK with remote data collection by Clinger, Jonathan A, Moreau, David W, McLeod, Matthew J, Holyoak, Todd, Thorne, Robert E

“…Time-resolved crystallography of biomolecules in action has advanced rapidly as methods for serial crystallography have improved, but the large number of…”
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Contact Line Pinning by Microfabricated Patterns: Effects of Microscale Topography by Kalinin, Yevgeniy V, Berejnov, Viatcheslav, Thorne, Robert E

“…We study how the microscale topography of a solid surface affects the apparent advancing and receding angles at the contact line of a liquid drop pinned to…”
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Effect of common cryoprotectants on critical warming rates and ice formation in aqueous solutions by Hopkins, Jesse B., Badeau, Ryan, Warkentin, Matthew, Thorne, Robert E.

“…Ice formation on warming is of comparable or greater importance to ice formation on cooling in determining survival of cryopreserved samples. Critical warming…”
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Ice formation and solvent nanoconfinement in protein crystals by Moreau, David W, Atakisi, Hakan, Thorne, Robert E

“…Ice formation within protein crystals is a major obstacle to the cryocrystallographic study of protein structure, and has limited studies of how the structural…”
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Lifetimes and spatio-temporal response of protein crystals in intense X-ray microbeams by Warkentin, Matthew A, Atakisi, Hakan, Hopkins, Jesse B, Walko, Donald, Thorne, Robert E

“…Serial synchrotron-based crystallography using intense microfocused X-ray beams, fast-framing detectors and protein microcrystals held at 300 K promises to…”
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Identifying structural and dynamic changes during the Biliverdin Reductase B catalytic cycle by Lee, Eunjeong, McLeod, Matthew J, Redzic, Jasmina S, Marcolin, Barbara, Thorne, Robert E, Agarwal, Pratul, Eisenmesser, Elan Zohar

“…Biliverdin Reductase B (BLVRB) is an NADPH-dependent reductase that catalyzes the reduction of multiple substrates and is therefore considered a critical…”
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Thermal contraction of aqueous glycerol and ethylene glycol solutions for optimized protein-crystal cryoprotection by Shen, Chen, Julius, Ethan F., Tyree, Timothy J., Moreau, David W., Atakisi, Hakan, Thorne, Robert E.

“…The thermal contraction of aqueous cryoprotectant solutions on cooling to cryogenic temperatures is of practical importance in protein cryocrystallography and…”
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Effects of cryoprotectant concentration and cooling rate on vitrification of aqueous solutions by Husseini, Naji S., Alsaied, Osama A., Thorne, Robert E., Berejnov, Viatcheslav

“…Vitrification of aqueous cryoprotectant mixtures is essential in cryopreservation of proteins and other biological samples. Systematic measurements of critical…”
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Active-site protein dynamics and solvent accessibility in native Achromobacter cycloclastes copper nitrite reductase by Sen, Kakali, Horrell, Sam, Kekilli, Demet, Yong, Chin W, Keal, Thomas W, Atakisi, Hakan, Moreau, David W, Thorne, Robert E, Hough, Michael A, Strange, Richard W

“…Microbial nitrite reductases are denitrifying enzymes that are a major component of the global nitrogen cycle. Multiple structures measured from one crystal…”
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Ice in biomolecular cryocrystallography by Moreau, David W., Atakisi, Hakan, Thorne, Robert E.

“…Diffraction data acquired from cryocooled protein crystals often include diffraction from ice. Analysis of ice diffraction from crystals of three proteins…”
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Density and electron density of aqueous cryoprotectant solutions at cryogenic temperatures for optimized cryoprotection and diffraction contrast by Tyree, Timothy J., Dan, Ritwik, Thorne, Robert E.

“…The glass‐phase densities at T = 77 K of aqueous solutions of the common cryoprotective agents (CPAs) methanol, ethanol, 2‐propanol, glycerol,…”
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