Search Results - "Thompson, David V"

Generation of site-specific retargeting platform cell lines for drug discovery using phiC31 and R4 integrases by Lieu, Pauline T, Machleidt, Thomas, Thyagarajan, Bhaskar, Fontes, Andrew, Frey, Elizabeth, Fuerstenau-Sharp, Maya, Thompson, David V, Swamilingiah, Geetha M, Derebail, Suchitra S, Piper, David, Chesnut, Jonathan D

“…One of the challenges in developing cell lines for high-throughput screening in drug discovery is the labor- and time-intensive process required to create…”
Get more information

High-throughput screening assays for the assessment of CYP2C91, CYP2C92, and CYP2C93 metabolism using fluorogenic Vivid substrates by Marks, Bryan D, Thompson, David V, Goossens, Tony A, Trubetskoy, Olga V

“…CYP2C9 is a genetically polymorphic human cytochrome P450 isozyme involved in the oxidative metabolism of many drugs, including nonsteroidal anti-inflammatory…”
Get more information

Development of the predictor HERG fluorescence polarization assay using a membrane protein enrichment approach by Piper, David R, Duff, Steve R, Eliason, Hildegard C, Frazee, W Jack, Frey, Elizabeth A, Fuerstenau-Sharp, Maya, Jachec, Christine, Marks, Bryan D, Pollok, Brian A, Shekhani, Mohammed Saleh, Thompson, David V, Whitney, Pam, Vogel, Kurt W, Hess, Stephen D

“…The life-threatening consequences of acquired, or drug-induced, long QT syndrome due to block of the human ether-a-go-go-related gene (hERG) channel are well…”
Get more information

Abstract 5595: Rapid and sensitive determination of cytokine release from cells without the need for sample transfer by Lazar, Dan F., Kupcho, Kevin R., Sondgeroth, Casey A., Thompson, David V., O'Brien, Martha A., Gilden, Julia K., Hsiao, Kevin, Cali, James J.

“…Abstract Cytokines play a major role in cancer biology as inflammatory and immunomodulatory agents and are frequently measured in cell culture models and other…”
Get full text

Rapid and sensitive determination of cytokine release from cells without the need for sample transfer by Lazar, Dan F, Kupcho, Kevin R, Sondgeroth, Casey A, Hsiao, Kevin, Thompson, David V, O’Brien, Martha A, Gilden, Julia K, Cali, James J

“…Abstract We have utilized NanoLuc® Binary Technology (NanoBiT®) to develop a homogeneous and rapid assay method (≤ 70 min completion time) to measure cytokines…”
Get full text

Reconstitution Premixes for Assays Using Purified Recombinant Human Cytochrome P450, NADPH-Cytochrome P450 Reductase, and Cytochromeb5 by Shaw, Peter M., Hosea, Natilie A., Thompson, David V., Lenius, Janean M., Guengerich, F.Peter

“…The development of enzyme and buffer premixes forin vitrobiotransformation assays is described. The protein premixes contain a mixture of three recombinant…”
Get full text

Molecular cloning and characterization of the insecticidal crystal protein gene of Bacillus thuringiensis var. tenebrionis by Sekar, V, Thompson, D.V, Maroney, M.J, Bookland, R.G, Adang, M.J

“…The insecticidal crystal protein gene of the coleopteran-toxic Bacillus thuringiensis var. tenebrionis has been isolated, and the nucleotide sequence has been…”
Get full text

Reconstitution premixes for assays using purified recombinant human cytochrome P450, NADPH-cytochrome P450 reductase, and cytochrome b5 by Shaw, P M, Hosea, N A, Thompson, D V, Lenius, J M, Guengerich, F P

“…The development of enzyme and buffer premixes for in vitro biotransformation assays is described. The protein premixes contain a mixture of three recombinant…”
Get full text

Perspectives in Experimental Botany. Sequence analysis of the vir-region from Agrobacterium tumefaciens octopine Ti plasmid pTi15955 by Schrammeijer, Barbara, Beijersbergen, Alice, Idler, Ken B, Melchers, Leo S, Thompson, David V, Paul J.J. Hooykaas

“…The nucleotide sequence of 42 775 bp of the vir-region from the Agrobacterium tumefaciens octopine Ti plasmid pTi15955 is reported here. Although the…”
Get full text

Sequence analysis of the vir‐region from Agrobacterium tumefaciens octopine Ti plasmid pTi15955 by Schrammeijer, Barbara, Beijersbergen, Alice, Idler, Ken B., Melchers, Leo S., Thompson, David V., Hooykaas, Paul J.J.

“…The nucleotide sequence of 42 775 bp of the vir‐region from the Agrobacterium tumefaciens octopine Ti plasmid pTi15955 is reported here. Although the…”
Get full text

Efficient site directed in vitro mutagenesis using ampicillin selection by LEWIS, M. K, THOMPSON, D. V

“…A novel plasmid vector pSELECT-1 is described which can be used for highly efficient site-directed in vitro mutagenesis. The mutagenesis method is based on the…”
Get full text

Characterized full-length and truncated plasmid clones of the crystal protein of Bacillus thuringiensis subsp. kurstaki HD-73 and their toxicity to Manduca sexta by Adang, M J, Staver, M J, Rocheleau, T A, Leighton, J, Barker, R F, Thompson, D V

“…Bacillus thuringiensis subsp. kurstaki HD-73 produces a crystal protein which is lethal to many lepidopteran larvae. The gene encoding this crystal protein has…”
Get more information

Nucieotide sequence of the maize transposable element Mul by Barker, Richard F., Thompson, David V., Talbot, Duncan R., Swanson, Jean, Bennetzen, Jeffrey L.

“…A cloned DNA fragment from the maize allele Adhl-S3034 contains all of Mul, an insertion element involved in Robertson's Mutator activity. The element is 1367…”
Get full text

Analysis of the complete nucleotide sequence of the Agrobacterium tumefaciens virB operon by THOMPSON, D. V, MELCHERS, L. S, IDLER, K. B, SCHILPEROORT, R. A, HOOYKAAS, P. J. J

“…The complete nucleotide sequence of the virB locus, from the octopine Ti plasmid of Agrobacterium tumefaciens strain 15955, has been determined. In the large…”
Get full text

Octopine and nopaline strains of Agrobacterium tumefaciens differ in virulence; molecular characterization of the virF locus by Melchers, L.S. (Leiden Univ. (Netherlands). Vakgroep Moleculaire Biologie van Planten), Maroney, M.J, Dulk-Ras, A. den, Thompson, D.V, Vuuren, H.A.J. van, Schilperoort, R.A, Hooykaas, P.J.J

“…Octopine and nopaline strains of Agrobacterium tumefaciens were found to differ in virulence on Nicotiana glauca. This difference is due to the absence of a…”
Get full text

Analysis of the complete nucleotide sequence of the Agrobacterium tumefaciens vir B operon by Thompson, David V., Melchers, Leo S., Idler, Ken B., Schilperoort, Rob A., Hooykaas, Paul J.J.

Get full text

Nucleotide sequence of two soybean ENOD2 early nodulin genes encoding Ngm-75 by Franssen, H.J. (Landbouwuniversiteit Wageningen (Netherlands). Vakgroep Moleculaire Biologie), Thompson, D.V, Idler, K, Kormelink, R, Kammen, A. van, Bisseling, T

“…Recently the isolation and characterization of the cDNA clone pGmENOD2 has been described. As it appeared of interest to identify the sequences on the plant…”
Get full text

In vitro sodium bisulfite mutagenesis of restriction endonuclease recognition sites by Merlo, D J, Thompson, D V

“…Sodium bisulfite treatment of single-stranded DNA deaminates exposed cytosine residues to form uracil, resulting in cytosine-to-thymidine transition mutations…”
Get more information