Antioxidant defense in a lead accumulating plant, Sesbania drummondii

Seedlings of Sesbania drummondii were grown in 500 mg l –1 Pb(NO 3) 2 in presence and absence of chelators: EDTA, DTPA and HEDTA for 4 weeks. Plants were assayed for activities of the antioxidant enzymes: ascorbate peroxidase (APX), guaiacol peroxidase (GPX), catalase (CAT), superoxide dismutase (SO...

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Bibliographic Details
Published in:	Plant physiology and biochemistry Vol. 42; no. 11; pp. 899 - 906
Main Authors:	Thomas Ruley, A., Sharma, Nilesh C., Sahi, Shivendra V.
Format:	Journal Article
Language:	English
Published:	Paris Elsevier Masson SAS 01-12-2004 Elsevier
Subjects:	Ascorbate peroxidase Ascorbate Peroxidases Biological and medical sciences Catalase Catalase - metabolism Chelating Agents - chemistry Chlorophyll - analysis Fabaceae - drug effects Fabaceae - enzymology Fabaceae - physiology Fundamental and applied biological sciences. Psychology Generalities. Disease free stocks Glutathione Guaiacol peroxidase Lead - toxicity Oxidative Stress - drug effects Oxidative Stress - physiology Peroxidase - metabolism Peroxidases - metabolism Photosynthesis Photosynthesis - drug effects Photosynthesis - physiology Phytopathology. Animal pests. Plant and forest protection Sesbania Superoxide dismutase Superoxide Dismutase - metabolism Sesbania Catalase CAT Guaiacol peroxidase Superoxide dismutase APX SOD Ascorbate peroxidase Photosynthesis GSH GPX Glutathione Oxidative stress Enzyme Growth Antioxidant Metabolism Chlorophyll a Leguminosae Peroxidases Dicotyledones Angiospermae Defense mechanism Spermatophyta Peroxidase Oxidoreductases Kinetics Ascorbate peroxidase: Catalase
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Summary:	Seedlings of Sesbania drummondii were grown in 500 mg l –1 Pb(NO 3) 2 in presence and absence of chelators: EDTA, DTPA and HEDTA for 4 weeks. Plants were assayed for activities of the antioxidant enzymes: ascorbate peroxidase (APX), guaiacol peroxidase (GPX), catalase (CAT), superoxide dismutase (SOD) and glutathione (γ-glutamyl-cysteinyl-glycine) content. Activities of antioxidant enzymes were elevated in the presence of Pb but were similar to controls in plants grown in the presence of Pb and EDTA, -DTPA or -HEDTA. Glutathione content was significantly elevated upon exposure to Pb, but lowered upon exposure to chelators. Chlorophyll a fluorescence kinetics were assessed by determination of F v/ F m and F v/ F o values. Seedling growth in Pb alone and Pb + chelators did not significantly affect photosynthetic integrity ( F v/ F o) and efficiency ( F v/ F m). The results suggest that Sesbania plants were able to tolerate Pb-induced stress using an effective antioxidant defense mechanism. This study also indicates a protective role of synthetic chelators in Pb-induced oxidative stress metabolism in a Pb-accumulating plant.
ISSN:	0981-9428 1873-2690
DOI:	10.1016/j.plaphy.2004.12.001