Portable Rabies Virus Sequencing in Canine Rabies Endemic Countries Using the Oxford Nanopore MinION

As countries with endemic canine rabies progress towards elimination by 2030, it will become necessary to employ techniques to help plan, monitor, and confirm canine rabies elimination. Sequencing can provide critical information to inform control and vaccination strategies by identifying geneticall...

Full description

Saved in:

Bibliographic Details
Published in:	Viruses Vol. 12; no. 11; p. 1255
Main Authors:	Gigante, Crystal M, Yale, Gowri, Condori, Rene Edgar, Costa, Niceta Cunha, Long, Nguyen Van, Minh, Phan Quang, Chuong, Vo Dinh, Tho, Nguyen Dang, Thanh, Nguyen Tat, Thin, Nguyen Xuan, Hanh, Nguyen Thi Hong, Wambura, Gati, Ade, Frederick, Mito, Oscar, Chuchu, Veronicah, Muturi, Mathew, Mwatondo, Athman, Hampson, Katie, Thumbi, Samuel M, Thomae, Byron G, de Paz, Victor Hugo, Meneses, Sergio, Munyua, Peninah, Moran, David, Cadena, Loren, Gibson, Andrew, Wallace, Ryan M, Pieracci, Emily G, Li, Yu
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI 04-11-2020 MDPI AG
Subjects:	Animals BASIC BIOLOGICAL SCIENCES canine rabies elimination Diagnostic Equipment Dog Diseases - virology Dogs Endemic Diseases - prevention & control Endemic Diseases - veterinary Guatemala Humans India Kenya lyssavirus MinION nanopore Nanopores Phylogeny portable sequencing Public Health rabies Rabies - veterinary Rabies - virology Rabies virus - classification Rabies virus - genetics Sequence Analysis, DNA - instrumentation Sequence Analysis, DNA - methods Vietnam Guatemala Vietnam Kenya India MinION canine rabies elimination rabies portable sequencing nanopore lyssavirus
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	As countries with endemic canine rabies progress towards elimination by 2030, it will become necessary to employ techniques to help plan, monitor, and confirm canine rabies elimination. Sequencing can provide critical information to inform control and vaccination strategies by identifying genetically distinct virus variants that may have different host reservoir species or geographic distributions. However, many rabies testing laboratories lack the resources or expertise for sequencing, especially in remote or rural areas where human rabies deaths are highest. We developed a low-cost, high throughput rabies virus sequencing method using the Oxford Nanopore MinION portable sequencer. A total of 259 sequences were generated from diverse rabies virus isolates in public health laboratories lacking rabies virus sequencing capacity in Guatemala, India, Kenya, and Vietnam. Phylogenetic analysis provided valuable insight into rabies virus diversity and distribution in these countries and identified a new rabies virus lineage in Kenya, the first published canine rabies virus sequence from Guatemala, evidence of rabies spread across an international border in Vietnam, and importation of a rabid dog into a state working to become rabies-free in India. Taken together, our evaluation highlights the MinION's potential for low-cost, high volume sequencing of pathogens in locations with limited resources.
Bibliography:	SC0014664 USDOE Office of Science (SC)
ISSN:	1999-4915 1999-4915
DOI:	10.3390/v12111255