Exome-informed formulations of food proteins enhance body growth and feed conversion efficiency in ad libitum-fed mice
[Display omitted] •The concept of protein quality includes defining intake targets of essential amino acids.•The genome-derive dietary EAA targets can be applied to other monogastric species.•Dietary protein matched to exome target improved feed conversion efficiency in mice.•Exome-matched feeds wit...
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| Published in: | Food research international Vol. 176; p. 113819 |
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| Main Authors: | , , , , , , , , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Canada
Elsevier Ltd
01-01-2024
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | [Display omitted]
•The concept of protein quality includes defining intake targets of essential amino acids.•The genome-derive dietary EAA targets can be applied to other monogastric species.•Dietary protein matched to exome target improved feed conversion efficiency in mice.•Exome-matched feeds with protein digestibility correction yielded further efficiency.•Different effects on lean to fat body mass ratios were observed for male and female mice.
Meeting requirements for dietary proteins, especially of essential amino acids (EAAs), is critical for the life-long health of living organisms. However, defining EAA targets for preparing biologically-matched nutrition that satisfies metabolic requirements for protein remains challenging. Previous research has shown the advantages of ‘exome matching’ in representing the specific requirement of dietary AAs, where the target dietary AA profile was derived from in silico translation of the genome of an organism, specifically responsible for protein expression (the ‘exome’). However, past studies have assessed these effects in only one sex, for few parameters (body mass and composition), and have used purified diets in which protein is supplied as a mixture of individual AAs. Here, for the first time, we utilise a computational method to guide the formulation of custom protein blends and test if exome matching can be achieved at the intact protein level, through blending standard protein ingredients, ultimately leading to optimal growth, longevity and reproductive function. Mice were provided ad libitum (ad lib) access to one of the four iso-energetic protein-limited diets, two matched and two mis-matched to the mouse exome target, and fed at a fixed protein energy level of 6.2%. During or following 13-weeks of feeding, the food intake, body growth, composition and reproductive functions were measured. Compared to the two mis-matched diets, male and female animals on the exome-matched diet with protein digestibility correction applied, exhibited significantly improved growth rates and final body mass. The feed conversion efficiency in the same diet was also increased by 62% and 40% over the worst diets for males and females, respectively. Male, not female, exhibited higher accretion of lean body mass with the matched, digestibility-corrected diet. All reproductive function measures in both sexes were comparable among diets, with the exception of testicular daily sperm production in males, which was higher in the two matched diets versus the mis-matched diets. The results collectively demonstrate the pronounced advantages of exome-matching in supporting body growth and improving feed conversion efficiency in both sexes. However, the potential impact of this approach in enhancing fertility needs further investigation. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 0963-9969 1873-7145 |
| DOI: | 10.1016/j.foodres.2023.113819 |