Permeability of the equine embryonic capsule to ethylene glycol and glycerol in vitro

Permeability of the equine embryonic capsule to ethylene glycol and glycerol in vitro

Poor survival of cryopreservation by equine expanded blastocysts may involve low penetration of the embryonic capsule by cryoprotective agents (CPAs). This study characterized the permeation and accumulation rates of the CPAs ethylene glycol (EG) and glycerol (GLY) across isolated capsule in vitro,...

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Published in:Theriogenology Vol. 76; no. 8; pp. 1540 - 1551
Main Authors: Kingma, S.E. Gillard, Thibault, M.E., Betteridge, K.J., Schlaf, M., Gartley, C.J., Chenier, T.S.
Format: Journal Article
Language:English
Published: United States Elsevier Inc 01-11-2011
Subjects:
Animals
Blastocyst - drug effects
Blastocyst - metabolism
Capsule
Cryopreservation
Cryopreservation - veterinary
Cryoprotectant
Cryoprotective Agents - pharmacology
Embryo
Equine
ethylene glycol
Ethylene Glycol - pharmacology
Female
gas chromatography
glycerol
Glycerol - pharmacology
horses
Horses - embryology
Permeability
sodium chloride
Embryo
Equine
Capsule
Cryopreservation
Cryoprotectant
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Summary:Poor survival of cryopreservation by equine expanded blastocysts may involve low penetration of the embryonic capsule by cryoprotective agents (CPAs). This study characterized the permeation and accumulation rates of the CPAs ethylene glycol (EG) and glycerol (GLY) across isolated capsule in vitro, using a dual-chambered Valia-Chien permeation apparatus. Pieces of Days 14 to 18 ±1 capsules separated media in the “donor” chamber containing either 1.5 M EG (n = 6), 0.74 M EG (n = 5), 0.87 M GLY (n = 7), or 0.15 M NaCl (saline, SAL) (n = 6), from the “recipient” chamber. Concentrations of CPA, determined by gas chromatography, allowed calculation of the capsule's apparent permeability (P app) to those CPAs. Permeation of capsule by 1.5 M EG was significantly more rapid than by 0.87 M GLY, or 0.74 M EG; permeation by both CPAs was significantly slower than by SAL. Accumulation of CPA in the recipient chamber depended more on initial donor chamber concentration, rather than type, of CPA. Accumulation rates for CPAs and SAL were linear only when capsule was present, demonstrating that their permeation through capsule was more complex than simple diffusion. Successful cryopreservation of equine expanded blastocysts has been previously linked to lengths of step-wise exposures to CPAs. Based on the present results, we inferred that alternative CPAs, more capable of permeating the capsule, or alternative methods of ensuring CPA entry into the cells, may also be required.
Bibliography:http://dx.doi.org/10.1016/j.theriogenology.2011.06.026
ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2011.06.026