Search Results - "Theodore L. Roth"

Microglia Development and Function by Nayak, Debasis, Roth, Theodore L, McGavern, Dorian B

“…Proper development and function of the mammalian central nervous system (CNS) depend critically on the activity of parenchymal sentinels referred to as…”
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Transcranial amelioration of inflammation and cell death after brain injury by Roth, Theodore L., Nayak, Debasis, Atanasijevic, Tatjana, Koretsky, Alan P., Latour, Lawrence L., McGavern, Dorian B.

“…Using long-term intravital photography to explore the cellular changes after compression-induced traumatic brain injury in a murine model, it is shown that…”
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Polymer-stabilized Cas9 nanoparticles and modified repair templates increase genome editing efficiency by Nguyen, David N., Roth, Theodore L., Li, P. Jonathan, Chen, Peixin Amy, Apathy, Ryan, Mamedov, Murad R., Vo, Linda T., Tobin, Victoria R., Goodman, Daniel, Shifrut, Eric, Bluestone, Jeffrey A., Puck, Jennifer M., Szoka, Francis C., Marson, Alexander

“…Versatile and precise genome modifications are needed to create a wider range of adoptive cellular therapies 1 – 5 . Here we report two improvements that…”
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The CD28-Transmembrane Domain Mediates Chimeric Antigen Receptor Heterodimerization With CD28 by Muller, Yannick D, Nguyen, Duy P, Ferreira, Leonardo M R, Ho, Patrick, Raffin, Caroline, Valencia, Roxxana Valeria Beltran, Congrave-Wilson, Zion, Roth, Theodore L, Eyquem, Justin, Van Gool, Frederic, Marson, Alexander, Perez, Laurent, Wells, James A, Bluestone, Jeffrey A, Tang, Qizhi

“…Anti-CD19 chimeric antigen receptor (CD19-CAR)-engineered T cells are approved therapeutics for malignancies. The impact of the hinge domain (HD) and the…”
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Single-molecule imaging of Hedgehog pathway protein Smoothened in primary cilia reveals binding events regulated by Patched1 by Milenkovic, Ljiljana, Lucien E. Weiss, Joshua Yoon, Theodore L. Roth, YouRong S. Su, Steffen J. Sahl, Matthew P. Scott, W. E. Moerner

“…Accumulation of the signaling protein Smoothened (Smo) in the membrane of primary cilia is an essential step in Hedgehog (Hh) signal transduction, yet the…”
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A functional map of HIV-host interactions in primary human T cells by Hiatt, Joseph, Hultquist, Judd F., McGregor, Michael J., Bouhaddou, Mehdi, Leenay, Ryan T., Simons, Lacy M., Young, Janet M., Haas, Paige, Roth, Theodore L., Tobin, Victoria, Wojcechowskyj, Jason A., Woo, Jonathan M., Rathore, Ujjwal, Cavero, Devin A., Shifrut, Eric, Nguyen, Thong T., Haas, Kelsey M., Malik, Harmit S., Doudna, Jennifer A., May, Andrew P., Marson, Alexander, Krogan, Nevan J.

“…Human Immunodeficiency Virus (HIV) relies on host molecular machinery for replication. Systematic attempts to genetically or biochemically define these host…”
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Migratory and adhesive cues controlling innate-like lymphocyte surveillance of the pathogen-exposed surface of the lymph node by Zhang, Yang, Roth, Theodore L, Gray, Elizabeth E, Chen, Hsin, Rodda, Lauren B, Liang, Yin, Ventura, Patrick, Villeda, Saul, Crocker, Paul R, Cyster, Jason G

“…Lymph nodes (LNs) contain innate-like lymphocytes that survey the subcapsular sinus (SCS) and associated macrophages for pathogen entry. The factors promoting…”
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XYZeq: Spatially resolved single-cell RNA sequencing reveals expression heterogeneity in the tumor microenvironment by Lee, Youjin, Bogdanoff, Derek, Wang, Yutong, Hartoularos, George C, Woo, Jonathan M, Mowery, Cody T, Nisonoff, Hunter M, Lee, David S, Sun, Yang, Lee, James, Mehdizadeh, Sadaf, Cantlon, Joshua, Shifrut, Eric, Ngyuen, David N, Roth, Theodore L, Song, Yun S, Marson, Alexander, Chow, Eric D, Ye, Chun Jimmie

“…Single-cell RNA sequencing (scRNA-seq) of tissues has revealed remarkable heterogeneity of cell types and states but does not provide information on the…”
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Robust T cell activation requires an eIF3-driven burst in T cell receptor translation by De Silva, Dasmanthie, Ferguson, Lucas, Chin, Grant H, Smith, Benjamin E, Apathy, Ryan A, Roth, Theodore L, Blaeschke, Franziska, Kudla, Marek, Marson, Alexander, Ingolia, Nicholas T, Cate, Jamie Hd

“…Activation of T cells requires a rapid surge in cellular protein synthesis. However, the role of translation initiation in the early induction of specific…”
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A rapid and simple method for DNA engineering using cycled ligation assembly by Roth, Theodore L, Milenkovic, Ljiljana, Scott, Matthew P

“…DNA assembly techniques have developed rapidly, enabling efficient construction of complex constructs that would be prohibitively difficult using traditional…”
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Epithelial miR-141 regulates IL-13-induced airway mucus production by Siddiqui, Sana, Johansson, Kristina, Joo, Alex, Bonser, Luke R, Koh, Kyung Duk, Le Tonqueze, Olivier, Bolourchi, Samaneh, Bautista, Rodriel A, Zlock, Lorna, Roth, Theodore L, Marson, Alexander, Bhakta, Nirav R, Ansel, K Mark, Finkbeiner, Walter E, Erle, David J, Woodruff, Prescott G

“…IL-13-induced goblet cell metaplasia contributes to airway remodeling and pathological mucus hypersecretion in asthma. miRNAs are potent modulators of cellular…”
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TCF-1 regulates HIV-specific CD8+ T cell expansion capacity by Rutishauser, Rachel L, Deguit, Christian Deo T, Hiatt, Joseph, Blaeschke, Franziska, Roth, Theodore L, Wang, Lynn, Raymond, Kyle A, Starke, Carly E, Mudd, Joseph C, Chen, Wenxuan, Smullin, Carolyn, Matus-Nicodemos, Rodrigo, Hoh, Rebecca, Krone, Melissa, Hecht, Frederick M, Pilcher, Christopher D, Martin, Jeffrey N, Koup, Richard A, Douek, Daniel C, Brenchley, Jason M, Sékaly, Rafick-Pierre, Pillai, Satish K, Marson, Alexander, Deeks, Steven G, McCune, Joseph M, Hunt, Peter W

“…Although many HIV cure strategies seek to expand HIV-specific CD8+ T cells to control the virus, all are likely to fail if cellular exhaustion is not…”
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Inflammation and neuroprotection in traumatic brain injury by Corps, Kara N, Roth, Theodore L, McGavern, Dorian B

“…Traumatic brain injury (TBI) is a significant public health concern that affects individuals in all demographics. With increasing interest in the medical and…”
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Light-activated cell identification and sorting (LACIS) for selection of edited clones on a nanofluidic device by Mocciaro, Annamaria, Roth, Theodore L., Bennett, Hayley M., Soumillon, Magali, Shah, Abhik, Hiatt, Joseph, Chapman, Kevin, Marson, Alexander, Lavieu, Gregory

“…Despite improvements in the CRISPR molecular toolbox, identifying and purifying properly edited clones remains slow, laborious, and low-yield. Here, we…”
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Genetic Disease and Therapy by Roth, Theodore L, Marson, Alexander

“…Genetic diseases cause numerous complex and intractable pathologies. DNA sequences encoding each human's complexity and many disease risks are contained in the…”
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Genome-wide CRISPR Screens in Primary Human T Cells Reveal Key Regulators of Immune Function by Shifrut, Eric, Carnevale, Julia, Tobin, Victoria, Roth, Theodore L., Woo, Jonathan M., Bui, Christina T., Li, P. Jonathan, Diolaiti, Morgan E., Ashworth, Alan, Marson, Alexander

“…Human T cells are central effectors of immunity and cancer immunotherapy. CRISPR-based functional studies in T cells could prioritize novel targets for drug…”
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Reprogramming human T cell function and specificity with non-viral genome targeting by Roth, Theodore L., Puig-Saus, Cristina, Yu, Ruby, Shifrut, Eric, Carnevale, Julia, Li, P. Jonathan, Hiatt, Joseph, Saco, Justin, Krystofinski, Paige, Li, Han, Tobin, Victoria, Nguyen, David N., Lee, Michael R., Putnam, Amy L., Ferris, Andrea L., Chen, Jeff W., Schickel, Jean-Nicolas, Pellerin, Laurence, Carmody, David, Alkorta-Aranburu, Gorka, del Gaudio, Daniela, Matsumoto, Hiroyuki, Morell, Montse, Mao, Ying, Cho, Min, Quadros, Rolen M., Gurumurthy, Channabasavaiah B., Smith, Baz, Haugwitz, Michael, Hughes, Stephen H., Weissman, Jonathan S., Schumann, Kathrin, Esensten, Jonathan H., May, Andrew P., Ashworth, Alan, Kupfer, Gary M., Greeley, Siri Atma W., Bacchetta, Rosa, Meffre, Eric, Roncarolo, Maria Grazia, Romberg, Neil, Herold, Kevan C., Ribas, Antoni, Leonetti, Manuel D., Marson, Alexander

“…Decades of work have aimed to genetically reprogram T cells for therapeutic purposes 1 , 2 using recombinant viral vectors, which do not target transgenes to…”
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CRISPR–Cas9 genome engineering of primary CD4+ T cells for the interrogation of HIV–host factor interactions by Hultquist, Judd F., Hiatt, Joseph, Schumann, Kathrin, McGregor, Michael J., Roth, Theodore L., Haas, Paige, Doudna, Jennifer A., Marson, Alexander, Krogan, Nevan J.

“…CRISPR–Cas9 gene-editing strategies have revolutionized our ability to engineer the human genome for robust functional interrogation of complex biological…”
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Pooled Knockin Targeting for Genome Engineering of Cellular Immunotherapies by Roth, Theodore L., Li, P. Jonathan, Blaeschke, Franziska, Nies, Jasper F., Apathy, Ryan, Mowery, Cody, Yu, Ruby, Nguyen, Michelle L.T., Lee, Youjin, Truong, Anna, Hiatt, Joseph, Wu, David, Nguyen, David N., Goodman, Daniel, Bluestone, Jeffrey A., Ye, Chun Jimmie, Roybal, Kole, Shifrut, Eric, Marson, Alexander

“…Adoptive transfer of genetically modified immune cells holds great promise for cancer immunotherapy. CRISPR knockin targeting can improve cell therapies, but…”
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A large CRISPR-induced bystander mutation causes immune dysregulation by Simeonov, Dimitre R., Brandt, Alexander J., Chan, Alice Y., Cortez, Jessica T., Li, Zhongmei, Woo, Jonathan M., Lee, Youjin, Carvalho, Claudia M. B., Indart, Alyssa C., Roth, Theodore L., Zou, James, May, Andrew P., Lupski, James R., Anderson, Mark S., Buaas, F. William, Rokhsar, Daniel S., Marson, Alexander

“…A persistent concern with CRISPR-Cas9 gene editing has been the potential to generate mutations at off-target genomic sites. While CRISPR-engineering mice to…”
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